M Amirul Islam, Ahasanul Karim, Puranjan Mishra et al.

The Science of the total environment • 2020

An understanding of the inter-species relationships, especially their metabolic network in a mixed-culture system, is crucial to design an effective inoculum for enhancing the power generation of wastewater fed microbial fuel cell (MFC). In the present study, the influence of microbial mutualistic interactions on the power generation of palm oil mill effluent fed MFCs has been widely investigated by designing several co-culture and mixed culture inoculums. Among the different inoculum compositions, the highest power density of 14.8 W/m3 was achieved by Pseudomonas aeruginosa and Klebsiella variicola co-culture inoculum due to their synergistic relationships which were inter-linked via fermentation-based metabolites. Besides, the interaction of K. variicola and Bacillus cereus positively influenced the power generation resulting in a maximum power density of 11.8 W/m3 whereas the antagonistic relationship between B. cereus and P. aeruginosa resulted in a lower power generation of 1.9 W/m3. The microbial mutualistic interactions were investigated with polarization, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), as well as by using metabolite and biofilm analysis. It was observed that the synergism between bacteria enhanced power generation through the production of higher electron shuttling mediators and efficient biofilm formation as evidenced by polarization, CV and EIS analysis. In contrast, the antagonistic relationship resulted in production of cell inhibiting metabolites leading to the formation of ineffective biofilm. These findings demonstrate that the synergistic interaction between or within microorganisms is emergent in designing co-culture or mixed-culture inoculum for achieving maximum power generation in MFCs.

Yue Lang, Yanan Yu, Hongtao Zou et al.

Chemosphere • 2021

Chemical park is regarded as a major contributor of VOCs emissions in China. Currently, a green and safe technology, microbial fuel cells (MFCs), is being developed for the VOCs abatement. Noting that effective electron transfer is critical to the MFC performance. In this work, flavin mononucleotide (FMN) was dosed as an electron shuttle to improve the removal of the typical toxic VOCs, toluene. The experimental results revealed that the performance of toluene removal and power generation were accelerated with the dosage of 0.2-2 μM FMN. With the addition of 1 μM FMN, the removal efficiency, the maximum output voltage and the coulombic efficiency of MFC were increased by 18.4%, 64.4% and 56.3%, respectively. However, a further increase in FMN concentration to 2 μM caused a reduction in the removal efficiency and coulombic efficiency. The images of scanning electron microscopy and confocal laser scanning microscopy showed that the presence of FMN greatly promoted the microbial growth and its activity. Furthermore, microbial community analysis also implied that the moderate dosage of FMN (0.2-1 μM) was beneficial for the growth of the typical exoelectrogens, Geobacter sp., and thus the coulombic efficiency was increased. In addition, an electron transfer pathway involving in cytochrome b, OMCs, cytochrome c, and MtrA was proposed based on the cyclic voltammetry analysis. This work will provide a fundamental theoretical support for its application of toxic VOCs abatement from the chemical park.

Xiaoya Zheng, Shanshan Hou, Charles Amanze et al.

Bioprocess and biosystems engineering • 2021

Low electricity generation efficiency is one of the key issues that must be addressed for the practical application of microbial fuel cells (MFCs). Modification of microbial electrode materials is an effective method to enhance electron transfer. In this study, magnetite (Fe3O4) nanoparticles synthesized by co-precipitation were added to anode chambers in different doses to explore its effect on the performance of MFCs. The maximum power density of the MFCs doped with 4.5 g/L Fe3O4 (391.11 ± 9.4 mW/m2) was significantly increased compared to that of the undoped MFCs (255.15 ± 24.8 mW/m2). The COD removal efficiency of the MFCs increased from 85.8 ± 2.8% to 95.0 ± 2.1%. Electrochemical impedance spectroscopy and cyclic voltammetry tests revealed that the addition of Fe3O4 nanoparticles enhanced the biocatalytic activity of the anode. High-throughput sequencing results indicated that 4.5 g/L Fe3O4 modified anodes enriched the exoelectrogen Geobacter (31.5%), while control MFCs had less Geobacter (17.4%). Magnetite is widely distributed worldwide, which provides an inexpensive means to improve the electrochemical performance of MFCs.

N F Shoparwe, M M Z Makhtar, S A Sata et al.

IOP Conference Series: Earth and Environmental Science • 2021

Abstract The present study aims to investigate the performance of batch culture of Geobacter sulfurreducens (G. sulfurreducens ) for electrical current generation via cyclic voltammetry (CV) method. The CV study was performed with an applied voltage in the range of -0.1 to 0.1 V against the standard calomel electrode (SCE) during the cell growth and attachment of G. sulfurreducens on graphite felt and initial acetate concentration of 20 mM. The kinetics of electrode reaction was investigated by conducting CV experiments at different scanning rates of 5, 10, 20, 50 and 100 mVs -1 . The diffusion coefficients (D) and heterogeneous electron transfer rate constant (k o ) of both anodic and cathodic process were 1.04×10 -5 cm 2 ·s -1 , 1.73×10 -6 cm 2 .s -1 , 0.0004 cm.s -1 and 0.0011 cm.s -1 , respectively. The obtained results showed that the anode exhibits high bioeletrocatalytic activity due to the attachment of G. sulfurreducens on the anode surface.

Miguel Ángel López Zavala, Omar Israel González Peña, Héctor Cabral Ruelas et al.

Energies • 2019

Cyclic voltammetry (CV) was used in this work to describe the electrochemical behavior of a dual-chamber microbial fuel cell (MFC). The system performance was evaluated under vacuum and non-pressurized conditions, different reaction times, two sweep potentials, 25 and 50 mVs−1 and under different analyte solutions, such as distilled water and domestic wastewater. CV experiments were conducted by using a potentiostat with three different configurations to collect the measurements. A dual-chamber MFC system was equipped with a DupontTM Nafion® 117 proton exchange membrane (PEM), graphite electrodes (8.0 cm × 2.5 cm × 0.2 cm) and an external electric circuit with a 100-Ω resistor. An electrolyte (0.1 M HCl, pH ≈ 1.8) was used in the cathode chamber. It was found that the proton exchange membrane plays a major role on the electrochemical behavior of the MFC when CV measurements allow observing the conductivity performance in the MFC in the absence of a reference electrode; under this potentiostat setting, less current density values are obtained on the scanned window potentials. Therefore, potentiostat setting is essential to obtain information in complex electrochemical processes present in biological systems, such as it is the case in the MFCs. Results of the study showed that wastewater constituents and the biomass suspended or attached (biofilm) over the electrode limited the electron charge transfer through the interface electrode-biofilm-liquor. This limitation can be overcome by: (i) Enhancing the conductivity of the liquor, which is a reduction of the ohmic drop, (ii) reducing the activation losses by a better catalysis, and (iii) by limiting the diffusional gradients in the bulk liquor, for instance, by forced convection. The use of the electrolyte (0.1 M HCl, pH ≈ 1.8) and its diffusion from the cathode to the anode chamber reduces the resistance to the flow of ions through the PEM and the flow of electrons through the anodic and cathodic electrolytes. Also reduces the activation losses during the electron transfer from the substrate to the electrode surface due to the electrode catalysis improvement. On the other hand, vacuum also demonstrated that it enhances the electrochemical performance of the dual-chamber MFC due to the fact that higher current densities in the system are favored.

Ryuhei Kishida

JOURNAL OF MECHANICS OF CONTINUA AND MATHEMATICAL SCIENCES • 2020

Derek R. Lovley

Microbiology • 2022

Geobacter metallireducens has served as the initial model for a substantial number of newly recognized microbial physiologies that play an important role in biogeochemical cycling of carbon, metals and nutrients. The strategies used by G. metallireducens for microbial interaction with minerals, contaminants, other microbes and electrodes have led to new technologies for bioremediation, bioenergy conversion and the sustainable production of ‘green’ electronics.

Ciana Lopez, Carlo Santoro, Plamen Atanassov et al.

ECS Meeting Abstracts • 2016

Bioelectrochemical systems (BESs) are interesting systems that combine electrochemical red-ox reaction with biological activity for generating electricity from organic compounds. In fact, the organic compounds are actually the fuel for the fuel cell in which bacteria on the anode degrade organic molecules and transfer the resulting electrons to the electrode surface. The electrons move through the external circuit generating useful electricity to power devices or sensors. At the cathode, an oxidant is reduced to complete the red-ox reaction. Generally oxygen is used due to its high potential and natural availability. Interestingly, it has been found that some bacteria, named exoelectrogens, are able to transfer electrons extracellularly to a solid support, generally called the anode electrode, if the substrate oxidation reaction occurs in absence of oxygen. The halfway potential represents the potential at which the electron transfer mechanism of an exoelectrogen is most favorable and thus outputs the most electricity. The more negative the halfway potential, the more energy can be produced. The goal of this project is to maximize microbial energy production considering different anode material-bacteria interactions. Carbonaceous-based materials are typically used as anode in BESs due to their simplicity, low-cost fabrication, high surface area, high mechanical strength, high chemical resistance to corrosion and biocompatibility. It has been shown previously that both surface chemistry and surface morphology can affect positively or negatively the bacteria attachment on a surface. Unfortunately, the electrical conductivity of carbonaceous materials is generally low compared to other materials and the durability is often negatively affected in long-term operation mainly due to material deterioration. Materials other than graphite have been proposed as suitable anode materials, but the effect of anode material on the underlying mechanism of extracellular electron transfer (EET) has not been yet addressed. Here, we measure electron transport properties of the model organism, Geobacter sulfurreducens , under turnover (with organic substrate) and nonturnover (without organic substrate) conditions, using an array of materials as working electrodes of an MFC (glassy carbon (GC), graphite (GR), gold (Au), platinum mesh (Pt), nickel (Ni) and indium tin oxide (ITO)). Experimentally, a 1L reactor that accommodates 6 working electrodes was used so all of the working electrodes could be tested under the same conditions with the same reference and counter electrodes. Ag/AgCl (3M KCl) was used as reference electrode while Pt was used as counter. Each material was used as a separate working electrode and connected to a single potentiostat (VMP3, Biologic, Inc., Knoxville, TN) channel. The reactor was operated using a three-electrode configuration at a set anode potential of +0.3 V (vs. Ag/AgCl) to study each material at stable fixed potential, as opposed to a floating potential observed for MFC anodes. Preliminary electrochemical tests produced cyclic voltammograms (CV) of all the materials under turnover and nonturnover conditions that displayed differences in slope and in the difference between halfway potential and formal potential, indicating that different materials yielded different electrochemical responses (Figure 1). The observed differences suggest that the bacteria are either using different electrochemical pathways to perform EET or that the material being used as the working electrode is influencing the environment and therefore altering the formal potential. We are currently conducting chemical measurements to characterize the working electrode surfaces along with a detailed study of the Geobacter biofilm colonization. Finally, we will establish a relationship between the halfway potential and extracellular electron transfer dependence on the surface to which the biofilm is attached. Figure 1. Polarization of Geobacter sulfurreducens grown for 14 days on various materials [Preliminary Data] Figure 1

Toshiyuki Ueki

Applied and Environmental Microbiology • 2021

Extracellular electron transfer (EET) is an important biological process in microbial physiology as found in dissimilatory metal oxidation/reduction and interspecies electron transfer in syntrophy in natural environments. EET also plays a critical role in microorganisms relevant to environmental biotechnology in metal-contaminated areas, metal corrosion, bioelectrochemical systems, and anaerobic digesters. Geobacter species exist in a diversity of natural and artificial environments.

Matthew J. Guberman-Pfeffer

Biophysical Journal • 2024

Daniel Härrer, Ahmed Elreedy, Rowayda Ali et al.

SSRN Electronic Journal • 2021

Rebecca J. Steidl, Sanela Lampa-Pastirk, Gemma Reguera

Nature Communications • 2017

Nature Communications 7: Artilce number: 12217 (2016); Published 2 August 2016; Updated 28 April 2017 Two previous studies (Vargas et al. 2013, Liu et al. 2014) reporting that conductive pili are required for long-range electron transport in Geobacter sulfurreducens were inadvertently omitted from the reference list of this Article, and should have been cited in the Introduction section where the possibility that pili function as biofilm electron carriers is discussed.

Rebecca J. Steidl, Sanela Lampa-Pastirk, Gemma Reguera

Nature Communications • 2016

Abstract Electricity generation by Geobacter sulfurreducens biofilms grown on electrodes involves matrix-associated electron carriers, such as c -type cytochromes. Yet, the contribution of the biofilm’s conductive pili remains uncertain, largely because pili-defective mutants also have cytochrome defects. Here we report that a pili-deficient mutant carrying an inactivating mutation in the pilus assembly motor PilB has no measurable defects in cytochrome expression, yet forms anode biofilms with reduced electroactivity and is unable to grow beyond a threshold distance (∼10 μm) from the underlying electrode. The defects are similar to those of a Tyr3 mutant, which produces poorly conductive pili. The results support a model in which the conductive pili permeate the biofilms to wire the cells to the conductive biofilm matrix and the underlying electrode, operating coordinately with cytochromes until the biofilm reaches a threshold thickness that limits the efficiency of the cytochrome pathway but not the functioning of the conductive pili network.

Guiqin Yang, Lingyan Huang, Lexing You et al.

Electrochemistry Communications • 2017

Jessica E. Butler, Richard H. Glaven, Abraham Esteve-Núñez et al.

Journal of Bacteriology • 2006

ABSTRACT The mechanism of fumarate reduction in Geobacter sulfurreducens was investigated. The genome contained genes encoding a heterotrimeric fumarate reductase, FrdCAB, with homology to the fumarate reductase of Wolinella succinogenes and the succinate dehydrogenase of Bacillus subtilis . Mutation of the putative catalytic subunit of the enzyme resulted in a strain that lacked fumarate reductase activity and was unable to grow with fumarate as the terminal electron acceptor. The mutant strain also lacked succinate dehydrogenase activity and did not grow with acetate as the electron donor and Fe(III) as the electron acceptor. The mutant strain could grow with acetate as the electron donor and Fe(III) as the electron acceptor if fumarate was provided to alleviate the need for succinate dehydrogenase activity in the tricarboxylic acid cycle. The growth rate of the mutant strain under these conditions was faster and the cell yields were higher than for wild type grown under conditions requiring succinate dehydrogenase activity, suggesting that the succinate dehydrogenase reaction consumes energy. An orthologous frdCAB operon was present in Geobacter metallireducens , which cannot grow with fumarate as the terminal electron acceptor. When a putative dicarboxylic acid transporter from G. sulfurreducens was expressed in G. metallireducens , growth with fumarate as the sole electron acceptor was possible. These results demonstrate that, unlike previously described organisms, G. sulfurreducens and possibly G. metallireducens use the same enzyme for both fumarate reduction and succinate oxidation in vivo.

Allison M. Speers, Gemma Reguera

Biofilm • 2021

Kelly P. Nevin, Dawn E. Holmes, Trevor L. Woodard et al.

International Journal of Systematic and Evolutionary Microbiology • 2005

Fe(III)-reducing isolates were recovered from two aquifers in which Fe(III) reduction is known to be important. Strain Bem T was enriched from subsurface sediments collected in Bemidji, MN, USA, near a site where Fe(III) reduction is important in aromatic hydrocarbon degradation. Strains P11, P35 T and P39 were isolated from the groundwater of an aquifer in Plymouth, MA, USA, in which Fe(III) reduction is important because of long-term inputs of acetate as a highway de-icing agent to the subsurface. All four isolates were Gram-negative, slightly curved rods that grew best in freshwater media. Strains P11, P35 T and P39 exhibited motility via means of monotrichous flagella. Analysis of the 16S rRNA and nifD genes indicated that all four strains are δ -proteobacteria and members of the Geobacter cluster of the Geobacteraceae . Differences in phenotypic and phylogenetic characteristics indicated that the four isolates represent two novel species within the genus Geobacter . All of the isolates coupled the oxidation of acetate to the reduction of Fe(III) [iron(III) citrate, amorphous iron(III) oxide, iron(III) pyrophosphate and iron(III) nitrilotriacetate]. All four strains utilized ethanol, lactate, malate, pyruvate and succinate as electron donors and malate and fumarate as electron acceptors. Strain Bem T grew fastest at 30 °C, whereas strains P11, P35 T and P39 grew equally well at 17, 22 and 30 °C. In addition, strains P11, P35 T and P39 were capable of growth at 4 °C. The names Geobacter bemidjiensis sp. nov. (type strain Bem T =ATCC BAA-1014 T =DSM 16622 T =JCM 12645 T ) and Geobacter psychrophilus sp. nov. (strains P11, P35 T and P39; type strain P35 T =ATCC BAA-1013 T =DSM 16674 T =JCM 12644 T ) are proposed.

Joana M. Dantas, Leonor Morgado, Muktak Aklujkar et al.

Frontiers in Microbiology • 2015

Shun'ichi Ishii, Kazuya Watanabe, Soichi Yabuki et al.

Applied and Environmental Microbiology • 2008

ABSTRACT An electricity-generating bacterium, Geobacter sulfurreducens PCA, was inoculated into a single-chamber, air-cathode microbial fuel cell (MFC) in order to determine the maximum electron transfer rate from bacteria to the anode. To create anodic reaction-limiting conditions, where electron transfer from bacteria to the anode is the rate-limiting step, anodes with electrogenic biofilms were reduced in size and tests were conducted using anodes of six different sizes. The smallest anode (7 cm 2 , or 1.5 times larger than the cathode) achieved an anodic reaction-limiting condition as a result of a limited mass of bacteria on the electrode. Under these conditions, the limiting current density reached a maximum of 1,530 mA/m 2 , and power density reached a maximum of 461 mW/m 2 . Per-biomass efficiency of the electron transfer rate was constant at 32 fmol cell −1 day −1 (178 μmol g of protein −1 min −1 ), a rate comparable to that with solid iron as the electron acceptor but lower than rates achieved with fumarate or soluble iron. In comparison, an enriched electricity-generating consortium reached 374 μmol g of protein −1 min −1 under the same conditions, suggesting that the consortium had a much greater capacity for electrode reduction. These results demonstrate that per-biomass electrode reduction rates (calculated by current density and biomass density on the anode) can be used to help make better comparisons of electrogenic activity in MFCs.

Feng Zhang, Shengsong Yu, Jie Li et al.

Frontiers of Environmental Science & Engineering • 2015

Dandan Deng, Yichi Zhang, Ying Liu

RSC Advances • 2014

A novel electrochemically active strain D-8 was successfully isolated from rice paddy soil. The strain D-8 can use more carbon sources and show higher current density than G. sulfurreducens PCA. It might be a promising bioanodic organism in MFCs.

Enrico Marsili, Jian Sun, Daniel R. Bond

Electroanalysis • 2010

Abstract The ability of Geobacter sulfurreducens to utilize electrodes as electron acceptors provides a system for monitoring mechanisms of electron transfer beyond the cell surface. This study examined the physiology of extracellular electron transfer during many stages of growth, and in response to short‐ and long‐term changes in electron acceptor potential. When G. sulfurreducens was grown on planar potentiostat‐controlled electrodes, the magnitude of early cell attachment increased with initial cell density. However, the first cells to attach did not demonstrate the same electron transfer rates as cells grown on electrodes. For example, following initial attachment of fumarate‐grown cells, the electron transfer rate was 2 mA/mg protein, but increased to nearly 8 mA/mg protein within 6 h of growth. Once attached, all biofilms grew at a constant rate (doubling every 6 h), and sustained a high specific electron transfer rate and growth yield, while current density was below 300 μA/cm 2 . Beyond this point, the rate of current increase slowed and approached a stable plateau. At all phases, slow scan rate cyclic voltammetry of G. sulfurreducens showed a similar well‐defined sigmoidal catalytic wave, indicating the general model of electron transfer to the electrode was not changing. Short‐term exposure to reducing potentials (3 h) did not alter these characteristics, but did cause accumulation of electrons which could be discharged at potentials above −0.1 V. Sustained growth at lower potentials (−0.16 V) only slightly altered the pattern of detectable redox species at the electrode, but did eliminate this pattern of discharge from the biofilm. Single‐turnover voltammetry of colonized electrodes showed at least 3 redox couples at potentials similar to other recent observations, with redox protein coverage of the electrode on the order of ca. 1 nmol/cm 2 . The consistent electrochemistry, growth rate, and growth yield of the G. sulfurreducens biofilm at all stages suggests an initial phase where cells must optimize attachment or electron transfer to a surface, and that after this point, the rate of electron production by cells (rate electrons are delivered to the external surface) remains rate limiting compared to the rate electrons can be transferred between cells, and to electrodes.

Douglas F. Call, Bruce E. Logan

Applied and Environmental Microbiology • 2011

ABSTRACT Geobacter sulfurreducens PCA completely oxidized lactate and reduced iron or an electrode, producing pyruvate and acetate intermediates. Compared to the current produced by Shewanella oneidensis MR-1, G. sulfurreducens PCA produced 10-times-higher current levels in lactate-fed microbial electrolysis cells. The kinetic and comparative analyses reported here suggest a prominent role of G. sulfurreducens strains in metal- and electrode-reducing communities supplied with lactate.

Pablo Sebastián Bonanni, Germán David Schrott, Juan Pablo Busalmen

Biochemical Society Transactions • 2012

The mechanism of electron transport in Geobacter sulfurreducens biofilms is a topic under intense study and debate. Although some proteins were found to be essential for current production, the specific role that each one plays in electron transport to the electrode remains to be elucidated and a consensus on the mechanism of electron transport has not been reached. In the present paper, to understand the state of the art in the topic, electron transport from inside of the cell to the electrode in Geobacter sulfurreducens biofilms is analysed, reviewing genetic studies, biofilm conductivity assays and electrochemical and spectro-electrochemical experiments. Furthermore, crucial data still required to achieve a deeper understanding are highlighted.

Hiroyuki Kashima, John M. Regan

Environmental Science & Technology • 2015

Alternative metabolic options of exoelectrogenic biofilms in bioelectrochemical systems (BESs) are important not only to explain the fundamental ecology and performance of these systems but also to develop reliable integrated nutrient removal strategies in BESs, which potentially involve substrates or intermediates that support/induce those alternative metabolisms. This research focused on dissimilatory nitrate reduction as an alternative metabolism to dissimilatory anode reduction. Using the exoelectrogenic nitrate reducer Geobacter metallireducens, the critical conditions controlling those alternative metabolisms were investigated in two-chamber, potentiostatically controlled BESs at various anode potentials and biofilm thicknesses and challenged over a range of nitrate concentrations. Results showed that anode-reducing biofilms facultatively reduced nitrate at all tested anode potentials (-150 to +900 mV vs Standard Hydrogen Electrode) with a rapid metabolic shift. The critical nitrate concentration that triggered a significant decrease in BES performance was a function of anode biofilm thickness but not anode potential. This indicates that these alternative metabolisms were controlled by the availability of nitrate, which is a function of nitrate concentration in bulk solution and its diffusion into an anode-reducing biofilm. Coulombic recovery decreased as a function of nitrate dose due to electron-acceptor substrate competition, and nitrate-induced suspended biomass growth decreased the effluent quality.

Jerome T. Babauta, Haluk Beyenal

ECS Meeting Abstracts • 2016

Model electrochemically active bacteria such as Geobacter sulfurreducens are well-established and are often used to study microbial interactions within biofilms that transfer electrons to electrodes. In the presented work, we utilized a quartz crystal microbalance (QCM) coupled to electrochemical impedance spectroscopy (EIS) to simultaneously monitor biofilm growth and the microbial interaction with the electrode. The QCM monitored the frequency shift from the background resonant frequency in real time while the current increased because of biofilm growth. At select times during biofilm growth, we halted the current and obtained biofilm impedance spectra. The short-term and long-term electrode interactions of G. sulfurreducens biofilms were demonstrated. In the short-term, the frequency shift was linear with respect to current for the biofilm. In long-term biofilm growth up to the exponential phase, a second linear region was observed. Biofilm impedance spectra taken across these times revealed a reproducible electrochemical signal. Conductance of the biofilm was linear with current whereas capacitance reached a limiting value towards the end of exponential growth. We show that a simple iV relationship can explain the linear behavior of conductance and current. Capacitance could be used to identify the transition between different growth phases. We compare the biofilm response on the QCM to capacitive, electrochemically-deposited polyaniline films. Our results suggest that the QCM can be used in applications where it is beneficial to identify electrochemically active bacteria that form efficient current-producing biofilms.

Merve TINGIR, Elif TARLAKAZAN

ODÜ Sosyal Bilimler Araştırmaları Dergisi (ODÜSOBİAD) • 2022

The original print painting has survived to the present day by changing with the progress of societies and time in the historical process. The development of technology has led to the use of the art of printmaking only for individual or educational purposes in schools. Today, 3D printing production techniques emerge as a new field in both design and production areas. Thanks to the convenience it offers, an alternative art creation tool emerges for designers. Using 3D printing with different disciplines, it creates diversity in the name of design and art. Thanks to the renewed technological developments, it contributes to its development by taking a new field into its sphere of influence every day. In the study, historical and general information about the art of printmaking is included, and the transition processes to 3D printing technology are discussed and compared. In addition, the transition processes and applications to 3D printing systems were examined. Qualitative research method will be used in the research. By making a literature review, the studies on the subject will be discussed and interpreted. This method covers the comparison of original print painting and 3D printing technology by referring to the point they have reached.

Arpine Galstyan, Michael J. Bunker, Fluvio Lobo et al.

3D Printing in Medicine • 2021

Abstract Three-dimensional (3D) printing is a method by which two-dimensional (2D) virtual data is converted to 3D objects by depositing various raw materials into successive layers. Even though the technology was invented almost 40 years ago, a rapid expansion in medical applications of 3D printing has only been observed in the last few years. 3D printing has been applied in almost every subspecialty of medicine for pre-surgical planning, production of patient-specific surgical devices, simulation, and training. While there are multiple review articles describing utilization of 3D printing in various disciplines, there is paucity of literature addressing applications of 3D printing in breast cancer management. Herein, we review the current applications of 3D printing in breast cancer management and discuss the potential impact on future practices.

Margaret Flavell, Cherie Chu-Fuluifaga

Teachers' Work • 2023

The low participation of Pacific students in tertiary STEM studies has implications for schools as they consider how best to engage these learners in STEM subjects (Science, Technology, Engineering and Mathematics). This article reports on an innovative project that supports Pacific learners with STEM learning through 3D printing technology. Creative STEM Pathways is a university-led initiative which has successfully brought 3D technology to the classroom, providing culturally-sustaining, hands-on and relevant learning opportunities. We used an Appreciative Inquiry lens to help us explore how the programme could create positive learning experiences. In this article, we share experiences of its development and delivery. We highlight successes and challenges, offering practical insight to those considering similar innovation in the classroom.

Shane Oberloier, Nicholas G. Whisman, Joshua M. Pearce

3D Printing and Additive Manufacturing • 2023

As additive manufacturing rapidly expands the number of materials including waste plastics and composites, there is an urgent need to reduce the experimental time needed to identify optimized printing parameters for novel materials. Computational intelligence (CI) in general and particle swarm optimization (PSO) algorithms in particular have been shown to accelerate finding optimal printing parameters. Unfortunately, the implementation of CI has been prohibitively complex for noncomputer scientists. To overcome these limitations, this article develops, tests, and validates PSO Experimenter, an easy-to-use open-source platform based around the PSO algorithm and applies it to optimizing recycled materials. Specifically, PSO Experimenter is used to find optimal printing parameters for a relatively unexplored potential distributed recycling and additive manufacturing (DRAM) material that is widely available: low-density polyethylene (LDPE). LDPE has been used to make filament, but in this study for the first time it was used in the open source fused particle fabrication/fused granular fabrication system. PSO Experimenter successfully identified functional printing parameters for this challenging-to-print waste plastic. The results indicate that PSO Experimenter can provide 97% reduction in research time for 3D printing parameter optimization. It is concluded that the PSO Experimenter is a user-friendly and effective free software for finding ideal parameters for the burgeoning challenge of DRAM as well as a wide range of other fields and processes.

Deborah L. Donohoe, Katherine Dennert, Rajeev Kumar et al.

3D Printing in Medicine • 2021

Abstract Background The ability of 3D printing using plastics and resins that are magnetic resonance imaging (MRI) compatible provides opportunities to tailor design features to specific imaging needs. In this study an MRI compatible cradle was designed to fit the need for repeatable serial images of mice within a mouse specific low field MRI. Methods Several designs were reviewed which resulted in an open style stereotaxic cradle to fit within specific bore tolerances and allow maximum flexibility with interchangeable radiofrequency (RF) coils. CAD drawings were generated, cradle was printed and tested with phantom material and animals. Images were analyzed for quality and optimized using the new cradle. Testing with multiple phantoms was done to affirm that material choice did not create unwanted image artifact and to optimize imaging parameters. Once phantom testing was satisfied, mouse imaging began. Results The 3D printed cradle fit instrument tolerances, accommodated multiple coil configurations and physiological monitoring equipment, and allowed for improved image quality and reproducibility while also reducing overall imaging time and animal safety. Conclusions The generation of a 3D printed stereotaxic cradle was a low-cost option which functioned well for our laboratory.

Magdalene Fogarasi, James C. Coburn, Beth Ripley

3D Printing in Medicine • 2022

Abstract Background 3D printing (3DP) has enabled medical professionals to create patient-specific medical devices to assist in surgical planning. Anatomical models can be generated from patient scans using a wide array of software, but there are limited studies on the geometric variance that is introduced during the digital conversion of images to models. The final accuracy of the 3D printed model is a function of manufacturing hardware quality control and the variability introduced during the multiple digital steps that convert patient scans to a printable format. This study provides a brief summary of common algorithms used for segmentation and refinement. Parameters for each that can introduce geometric variability are also identified. Several metrics for measuring variability between models and validating processes are explored and assessed. Methods Using a clinical maxillofacial CT scan of a patient with a tumor of the mandible, four segmentation and refinement workflows were processed using four software packages. Differences in segmentation were calculated using several techniques including volumetric, surface, linear, global, and local measurements. Results Visual inspection of print-ready models showed distinct differences in the thickness of the medial wall of the mandible adjacent to the tumor. Volumetric intersections and heatmaps provided useful local metrics of mismatch or variance between models made by different workflows. They also allowed calculations of aggregate percentage agreement and disagreement which provided a global benchmark metric. For the relevant regions of interest (ROIs), statistically significant differences were found in the volume and surface area comparisons for the final mandible and tumor models, as well as between measurements of the nerve central path. As with all clinical use cases, statistically significant results must be weighed against the clinical significance of any deviations found. Conclusions Statistically significant geometric variations from differences in segmentation and refinement algorithms can be introduced into patient-specific models. No single metric was able to capture the true accuracy of the final models. However, a combination of global and local measurements provided an understanding of important geometric variations. The clinical implications of each geometric variation is different for each anatomical location and should be evaluated on a case-by-case basis by clinicians familiar with the process. Understanding the basic segmentation and refinement functions of software is essential for sites to create a baseline from which to evaluate their standard workflows, user training, and inter-user variability when using patient-specific models for clinical interventions or decisions.

Orly Talyosef

Architext • 2020

Three-dimensional (3D) printing, also called additive manufacture (AM), is a novel, automated method of printing a structure layer-by-layer directly from a 3D digital design model. Its potential ability to build complex shapes in a less costly and more sustainable manner may revolutionize the construction industry. There are three main 3D printing techniques: (a) contour crafting; (b) concrete printing, and (c) D-shape. As a disruptive technology, 3D printing creates a new market and value network, thus disturbing the established market. Building information modeling (BIM) is a comprehensive management approach encompassing the entire life cycle of the architecture and construction (A&C) process, including architectural planning, geometrical data, scheduling, material, equipment, resource and manufacturing data, and post-construction facility management. By maintaining safety and productivity in large-scale digital processes, BIM is critical to 3D printing’s success in construction. Integrating BIM and 3D printing techniques into A&C can potentially lead to an ecological architectural process that reduces waste and energy inefficiency, and prevents injuries and fatalities on construction sites, while increasing productivity and quality. This paper examines BIM-based 3D printing of sustainable buildings, which may revolutionize the construction industry and contribute to a sustainable environment

Jana Macháčková, Alena Komersová, Marie Nevyhoštěná et al.

3D Printing and Additive Manufacturing • 2024

Naomi C. Paxton

3D Printing in Medicine • 2023

Ayşegül ASLAN, Yaren ÇELİK

International Journal of 3D Printing Technologies and Digital Industry • 2022

This study will offer domestic and foreign studies on the application of 3D printing technologies in the fields of education. The aim of this study was to review the studies that had been done in the literature on the application of 3D printing technology in the field of education. Purposive sampling method was used in the study. In this context, it was decided that in the current study, variables such as the distribution of studies on the use of 3D printer technology in the field of education by years, publication types, sample types and sizes, data collection tools and analysis methods were planned to be examined, and at the same time, in-depth analysis of the results was the most appropriate method. 101 studies were accessed in accordance with this research. One of the qualitative research methods used in the study was document analysis, and the data was analysed by examining at the documents that contained details regarding the facts that were the focus of the study. According to the data obtained, it is seen that the studies carried out on 3D printing technology between 2009-2022 are within the scope of educational activities at the K-12 level (physics, chemistry, biology, mathematics) and their numbers have increased especially after 2017. It was determined that undergraduate students were preferred the most as the sample group. It was found that, on general, qualitative research methodologies were preferred in the studies under consideration. It was seen that in-class assessments, observations and questionnaires were mostly used as data collection tools. It has been determined that content analysis is generally used in the analysis of the collected data. When the relationship between education and 3D printing technology was examined, it was determined that it can be adapted to all ages and fields and provides great convenience in interdisciplinary studies. Based on these findings, it is thought that it will be more effective to focus on the instructional aspect of 3D printing technology.

Pieter De Backer, Charlotte Allaeys, Charlotte Debbaut et al.

3D Printing in Medicine • 2021

Abstract Background Carotid Artery Stenting (CAS) is increasingly being used in selected patients as a minimal invasive approach to carotid endarterectomy. Despite the long standing tradition of endovascular treatments, visual feedback during stent-deployment is impossible to obtain as deployment is performed under fluoroscopic imaging. Furthermore, the concept of stent-placement is often still unclear to patients. 3D Printing allows to replicate patient-specific anatomies and deploy stents inside them to simulate procedures. As such these models are being used for endovascular training as well as patient education. Purpose To our knowledge, this study reports the first use of a low-cost patient-specific 3D printed model for teaching CAS deployment under direct visualization, without fluoroscopy. Methodology A CT-angiogram was segmented and converted to STL format using Mimics inPrint™ software. The carotid arteries were bilaterally truncated to fit the whole model on a Formlabs 2 printer without omitting the internal vessel diameter. Next, this model was offset using a 1 mm margin. A ridge was modelled on the original vessel anatomy which was subsequently subtracted from the offset model in order to obtain a deroofed 3D model. All vessels were truncated to facilitate post-processing, flow and guide wire placement. Results Carotid artery stents were successfully deployed inside the vessel. The deroofing allows for clear visualization of the bottlenecks and characteristics of CAS deployment and positioning, including stent foreshortening, tapering and recoil. This low-cost 3D model provides visual insights in stent deployment and positioning, and can allow for patient-specific procedure planning. Conclusions The presented approach demonstrates the use of low-cost 3D Printed CAS models in teaching complex stent behavior as observed during deployment. Two main findings are illustrated. On one hand, the feasibility of low-cost in-hospital model production is shown. On the other hand, the teaching of CAS deployment bottlenecks at the carotid level without the need for fluoroscopic guidance, is illustrated. The observed stent characteristics as shown during deployment are difficult to assess in radiologic models. Furthermore, printing patient-specific 3D models preoperatively could possibly assist in accurate patient selection, preoperative planning, case-specific training and patient education.

Carly M. Cooke, Teresa E. Flaxman, Lindsey Sikora et al.

3D Printing in Medicine • 2023

Abstract Objective Developments in 3-dimensional (3D) printing technology has made it possible to produce high quality, affordable 3D printed models for use in medicine. As a result, there is a growing assessment of this approach being published in the medical literature. The objective of this study was to outline the clinical applications of individualized 3D printing in gynecology through a scoping review. Data sources Four medical databases (Medline, Embase, Cochrane CENTRAL, Scopus) and grey literature were searched for publications meeting eligibility criteria up to 31 May 2021. Study eligibility criteria Publications were included if they were published in English, had a gynecologic context, and involved production of patient specific 3D printed product(s). Study appraisal and synthesis methods Studies were manually screened and assessed for eligibility by two independent reviewers and data were extracted using pre-established criteria using Covidence software. Results Overall, 32 studies (15 abstracts,17 full text articles) were included in the scoping review. Most studies were either case reports (12/32,38%) or case series (15/32,47%). Gynecologic sub-specialties in which the 3D printed models were intended for use included: gynecologic oncology (21/32,66%), benign gynecology (6/32,19%), pediatrics (2/32,6%), urogynecology (2/32,6%) and reproductive endocrinology and infertility (1/32,3%). Twenty studies (63%) printed 5 or less models, 6/32 studies (19%) printed greater than 5 (up to 50 models). Types of 3D models printed included: anatomical models (11/32,34%), medical devices, (2/32,6%) and template/guide/cylindrical applicators for brachytherapy (19/32,59%). Conclusions Our scoping review has outlined novel clinical applications for individualized 3D printed models in gynecology. To date, they have mainly been used for production of patient specific 3D printed brachytherapy guides/applicators in patients with gynecologic cancer. However, individualized 3D printing shows great promise for utility in surgical planning, surgical education, and production of patient specific devices, across gynecologic subspecialties. Evidence supporting the clinical value of individualized 3D printing in gynecology is limited by studies with small sample size and non-standardized reporting, which should be the focus of future studies.

Yu-Hui Huang, Bonnie Lee, Jeffrey A. Chuy et al.

3D Printing in Medicine • 2022

Abstract Background Advanced diagnostic imaging is an essential part of preoperative planning for oral and maxillofacial surgery in veterinary patients. 3-dimensional (3D) printed models and surgical guides generated from diagnostic imaging can provide a deeper understanding of the complex maxillofacial anatomy, including relevant spatial relationships. Additionally, patient-specific 3D printed models allow surgeons and trainees to better examine anatomical features through tactile and visuospatial feedback allowing for improved preoperative planning, intraoperative guidance, and enhanced trainee education. Furthermore, these models facilitate discussions with pet owners, allowing for improved owner understanding of pathology, and educated decision-making regarding treatment. Case presentation Our case series consists of three 3D printed models segmented from computed tomography (CT) and cone beam CT (CBCT) and fabricated via desktop vat polymerization for preoperative planning and intraoperative guidance for resection of maxillary osteosarcoma, mandibular reconstruction after mandibulectomy, and gap arthroplasty for temporomandibular joint ankylosis in dogs. Conclusions We illustrate multiple benefits and indications for 3D printing in veterinary oral and maxillofacial surgery. 3D printed models facilitate the understanding of complex surgical anatomy, creating an opportunity to assess the spatial relationship of the relevant structures. It facilitates individualized surgical planning by allowing surgeons to tailor and augment the surgical plan by examining patient-specific anatomy and pathology. Surgical steps may also be simulated in advance, including planning of osteotomy lines, and pre-contouring of titanium plates for reconstruction. Additionally, a 3D printed model and surgical guide also serve as invaluable intraoperative reference and guidance. Furthermore, 3D printed models have the potential to improve veterinary resident and student training as well as pet owner understanding and communication regarding the condition of their pets, treatment plan and intended outcomes.

Naomi C. Paxton

3D Printing in Medicine • 2023

Abstract 3D printing technology has become increasingly popular in healthcare settings, with applications of 3D printed anatomical models ranging from diagnostics and surgical planning to patient education. However, as the use of 3D printed anatomical models becomes more widespread, there is a growing need for regulation and quality control to ensure their accuracy and safety. This literature review examines the current state of 3D printing in hospitals and FDA regulation process for software intended for use in producing 3D printed models and provides for the first time a comprehensive list of approved software platforms alongside the 3D printers that have been validated with each for producing 3D printed anatomical models. The process for verification and validation of these 3D printed products, as well as the potential for inaccuracy in these models, is discussed, including methods for testing accuracy, limits, and standards for accuracy testing. This article emphasizes the importance of regulation and quality control in the use of 3D printing technology in healthcare, the need for clear guidelines and standards for both the software and the printed products to ensure the safety and accuracy of 3D printed anatomical models, and the opportunity to expand the library of regulated 3D printers.