[object Object], [object Object], [object Object] et al.

Applied Sciences • 2026

Microalgal-bacterial consortium is an effective way to meet increasingly stringent standards in wastewater treatment. However, the mechanism of wastewater removal effect has not been properly explained in community structure by phycosphere. And little is known about that the concept of macroecology was introduced into phycosphere to explain the phenomenon. In the study, the algal-bacterial consortia with different ratios of algae and sludge were cultured in same aerobic wastewater within 48 h in photobioreactors (PSBRs). Community structure at start and end was texted by metagenomic analysis. Bray-Curtis similarities analysis based on microbial community showed that there was obvious convergent succession in all consortia, which is well known as "convergence" in macroecology. The result showed that Bray-Curtis similarities at End (overall above 0.88) were higher than these at Start (almost less than 0.66). In terms of community structure, the consortium with 5:1 ratio at Start are the more similar with the consortia at End by which the maximum removal of total dissolved nitrogen (TDN, 73.69%), total dissolved phosphorus (TDP, 94.40%) and NH 3 -N (93.26%) in wastewater treatment process and biomass production (98.2%) higher than other consortia, according with climax community in macroecology with the highest resource utilization than other communities. Therefore, the macroecology can be introduced into phycosphere to explain the consortium for advanced wastewater treatment and optimization community structure. And the study revealed a novel insight into treatment effect and community structure of algal-bacterial consortia for advanced wastewater treatment, a new idea for to shortening the culture time of consortium and optimize predicting their ecological community structure and predicting ecological community.

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npj Biofilms and Microbiomes • 2020

Understanding of the extreme microorganisms that possess extracellular electron transfer (EET) capabilities is pivotal to advance electromicrobiology discipline and to develop niche-specific microbial electrochemistry-driven biotechnologies. Here, we report on the microbial electroactive biofilms (EABs) possessing the outward EET capabilities from a haloalkaline environment of the Lonar lake. We used the electrochemical cultivation approach to enrich haloalkaliphilic EABs under 9.5 pH and 20 g/L salinity conditions. The electrodes controlled at 0.2 V vs. Ag/AgCl yielded the best-performing biofilms in terms of maximum bioelectrocatalytic current densities of 548 ± 23 and 437 ± 17 µA/cm 2 with acetate and lactate substrates, respectively. Electrochemical characterization of biofilms revealed the presence of two putative redox-active moieties with the mean formal potentials of 0.183 and 0.333 V vs. Ag/AgCl, which represent the highest values reported to date for the EABs. 16S-rRNA amplicon sequencing of EABs revealed the dominance of unknown Geoalkalibacter sp. at ~80% abundance. Further investigations on the haloalkaliphilic EABs possessing EET components with high formal potentials might offer interesting research prospects in electromicrobiology.

[object Object], [object Object], [object Object] et al.

Nature Communications • 2019

Carbon supported PtCo intermetallic alloys are known to be one of the most promising candidates as low-platinum oxygen reduction reaction electrocatalysts for proton-exchange-membrane fuel cells. Nevertheless, the intrinsic trade-off between particle size and ordering degree of PtCo makes it challenging to simultaneously achieve a high specific activity and a large active surface area. Here, by machine-learning-accelerated screenings from the immense configuration space, we are able to statistically quantify the impact of chemical ordering on thermodynamic stability. We find that introducing of Cu/Ni into PtCo can provide additional stabilization energy by inducing Co-Cu/Ni disorder, thus facilitating the ordering process and achieveing an improved tradeoff between specific activity and active surface area. Guided by the theoretical prediction, the small sized and highly ordered ternary Pt 2 CoCu and Pt 2 CoNi catalysts are experimentally prepared, showing a large electrochemically active surface area of ~90 m 2 g Pt ‒1 and a high specific activity of ~3.5 mA cm ‒2 .

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Applied Chemical Engineering • 2024

The availability of renewable energy technologies is increasing dramatically across the globe thanks to their growing maturity. However, large scale electrical energy storage and retrieval will almost certainly be a required in order to raise the penetration of renewable sources into the grid. No present energy storage technology has the perfect combination of high power and energy density, low financial and environmental cost, lack of site restrictions, long cycle and calendar lifespan, easy materials availability, and fast response time. Engineered electroactive microbes could address many of the limitations of current energy storage technologies by enabling rewired carbon fixation, a process that spatially separates reactions that are normally carried out together in a photosynthetic cell and replaces the least efficient with non-biological equivalents. If successful, this could allow storage of renewable electricity through electrochemical or enzymatic fixation of carbon dioxide and subsequent storage as carbon-based energy storage molecules including hydrocarbons and non-volatile polymers at high efficiency. In this article we compile performance data on biological and non-biological component choices for rewired carbon fixation systems and identify pressing research and engineering challenges.

[object Object], [object Object], [object Object] et al.

Egyptian Liver Journal • 2024

Hepatocellular carcinoma (HCC, accounting for 90% of primary liver cancer) was the sixth most common cancer in the world and the third leading cause of cancer death in 2020. The number of new HCC patients in China accounted for nearly half of that in the world. HCC was of occult and complex onset, with poor prognosis. Clinically, at least 15% of patients with HCC had strong side effects of interventional therapy (IT) and have poor sensitivity to chemotherapy and targeted therapy. Traditional Chinese medicine (TCM), as a multi-target adjuvant therapy, had been shown to play an active anti-tumor role in many previous studies. This review systematically summarized the role of TCM combined with clinically commonly used drugs for the treatment of HCC (including mitomycin C, cyclophosphamide, doxorubicin, 5-fluorouracil, sorafenib, etc.) in the past basic research, and summarized the efficacy of TCM combined with surgery, IT and conventional therapy (CT) in clinical research. It was found that TCM, as an adjuvant treatment, played many roles in the treatment of HCC, including enhancing the tumor inhibition, reducing toxic and side effects, improving chemosensitivity and prolonging survival time of patients. This review summarized the advantages of integrated traditional Chinese and modern medicine in the treatment of HCC and provides a theoretical basis for clinical research.

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[object Object], [object Object], [object Object] et al.

Polymers • 2019

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[object Object], [object Object], [object Object] et al.

Frontiers in Microbiology • 2022

[object Object], [object Object], [object Object] et al.

Farming System • 2023

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[object Object], [object Object]

Sensors • 2009

[object Object], [object Object], [object Object] et al.

International Journal of Molecular Sciences • 2023

Microbial electrosynthesis (MES) is a sustainable approach to address greenhouse gas (GHG) emissions using anthropogenic carbon dioxide (CO 2 ) as a building block to create clean fuels and highly valuable chemicals. The efficiency of MES-based CO 2 conversion is closely related to the performance of electrode material and, in particular, the cathode for which carbonaceous materials are frequently used. Compared to expensive metal electrodes, carbonaceous materials are biocompatible with a high specific surface area, wide range of possible morphologies, and excellent chemical stability, and their use can maximize the growth of bacteria and enhance electron transfer rates. Examples include MES cathodes based on carbon nanotubes, graphene, graphene oxide, graphite, graphite felt, graphitic carbon nitride (g-C 3 N 4 ), activated carbon, carbon felt, carbon dots, carbon fibers, carbon brushes, carbon cloth, reticulated vitreous carbon foam, MXenes, and biochar. Herein, we review the state-of-the-art MES, including thermodynamic and kinetic processes that underpin MES-based CO 2 conversion, as well as the impact of reactor type and configuration, selection of biocompatible electrolytes, product selectivity, and the use of novel methods for stimulating biomass accumulation. Specific emphasis is placed on carbonaceous electrode materials, their 3D bioprinting and surface features, and the use of waste-derived carbon or biochar as an outstanding material for further improving the environmental conditions of CO 2 conversion using carbon-hungry microbes and as a step toward the circular economy. MES would be an outstanding technique to develop rocket fuels and bioderived products using CO 2 in the atmosphere for the Mars mission.

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The exoelectrogenic capacity of the cyanobacterium Synechococcus elongatus PCC7942 was studied in iron limited growth in order to establish conditions favouring extracellular electron transfer in cyanobacteria for photo-bioelectricity generation. Investigation into extracellular reduction of ferricyanide by Synechococcus elongatus PCC7942 demonstrated enhanced capability for the iron limited conditions in comparison to the iron sufficient conditions. Furtheremore, the significance of pH showed that higher rates of ferricyanide reduction occurred at pH 7, with a 2.7-fold increase with respect to pH 9.5 for iron sufficient cultures and 24-fold increase for iron limited cultures. The strategy presented induced exoelectrogenesis driven mainly by photosynthesis and an estimated redirection of the 28% of electrons from photosynthetic activity was achieved by the iron limited conditions. In addition, ferricyanide reduction in the dark by iron limited cultures also presented a significant improvement, with a 6-fold increase in comparison to iron sufficient cultures. Synechococcus elongatus PCC7942 ferricyanide reduction rates are unprecedented for cyanobacteria and they are comparable to those of microalgae. The redox activity of biofilms directly on ITO-coated glass, in the absence of any artificial mediator, was also enhanced under the iron limited conditions, implying that iron limitation increased exoelectrogenesis at the outer membrane level. Cyclic voltammetry of Synechococcus elongatus PCC7942 biofilms on ITO-coated glass showed a midpoint potential around 0.22 V vs. Ag/AgCl and iron limited biofilms had the capability to sustain currents in a saturated-like fashion. The present work proposes an iron related exoelectrogenic capacity of Synechococcus elongatus PCC7942 and sets a starting point for the study of this strain in order to improve photo-bioelectricity and dark-bioelectricity generation by cyanobacteria, including more sustainable mediatorless systems.

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[object Object], [object Object], [object Object] et al.

Green Chemistry • 2015

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Environmental Research Engineering and Management • 2020

[object Object], [object Object], [object Object] et al.

Environmental Pollution and Protection • 2017

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Pharmaceutica Analytica Acta • 2018

[object Object], [object Object], [object Object] et al.

Clinical Oral Investigations • 2023

Considering that environmental risk factors substantially contribute to the etiology of orofacial clefts and that knowledge about the characteristics and comorbidities associated with oral clefts is fundamental to promoting better quality of life, this study aimed to describe the risk factors, main characteristics, and comorbidities of a group of patients with cleft lip and/or cleft palate (CL/P) from Rio Grande do Norte (RN), Brazil. Data were obtained from 173 patients with CL/P using a form from the Brazilian database on Orofacial Clefts. Most patients were male with cleft lip and palate and had a normal size and weight at birth; presented few neonatal intercurrent events; and had anemia and respiratory and cardiovascular diseases as main associated comorbidities. They also required timely surgical rehabilitation and multidisciplinary care to stimulate their neuropsychomotor development. In addition, a high frequency of familial recurrence and of parental consanguinity was evidenced in the studied population, especially for the cleft lip and cleft palate type. Other relevant findings were the considerable maternal exposure to alcohol, infections, smoking, and hypertension, as well as low supplementation with vitamins and minerals and deliberate consumption of analgesics, antibiotics, and antihypertensives during pregnancy. Characterization of the CL/P patient profile is essential for the planning of health services and integration among the health professionals involved in the diagnosis and treatment of these malformations. Our results reinforce the need for additional research to confirm the association between environmental factors and the development of orofacial clefts.

[object Object], [object Object], [object Object]

Nanoscale • 2018

[object Object], [object Object], [object Object] et al.

Scientific Reports • 2020

Microbial electrolysis cells (MEC) can produce hydrogen (H 2 ) at a low energy expense, but the H 2 production rate is often limited by poor microbe-electrode interaction. This study aimed to enhance the interaction of microbes with a cathode electrode modified with an iron-sulfide (FeS) catalyst in MECs to achieve an efficient H 2 evolution reaction (HER) and to investigate performance at different substrate concentrations, ranging from 1 to 3 g/L of glucose. The electrochemical analysis revealed FeS, a highly active catalyst for HER, surpassing the performance of a 10% platinum (Pt-C)-modified cathode. At 2 g/L glucose, MECs with a FeS-modified cathode (MEC-FeS) produced H 2 at the highest yield of 7.01 mol H 2 /mol glucose, and the H 2 production rate was 1.96 ± 0.09 m 3 /m 3 ·d. The control operations of MEC with a pristine cathode and dark fermentation resulted in a reduced H 2 yield of 5.83 ± 0.49 mol H 2 /mol glucose and 2.12 ± 0.12 mol H 2 /mol glucose, respectively. Moreover, the MEC-FeS achieved a high energy efficiency of 78 ± 5% compared to the MEC without catalyst (60 ± 5%) and the dark fermentation (24 ± 1%). This study suggests that utilizing FeS as a cathode catalyst in MECs can ensure high-rate hydrogen generation with optimal substrate concentration, paving the way for efficient upscaling and field application.

[object Object], [object Object], [object Object] et al.

Agronomy for Sustainable Development • 2023

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[object Object], [object Object], [object Object] et al.

Biotechnology for Biofuels • 2021

It is desirable to improve the anaerobic digestion processes of recalcitrant materials, such as cellulose. Enhancement of methane (CH 4 ) production from organic molecules was previously accomplished through coupling a bioelectrochemical system (BES); however, scaling-up BES-based production is difficult. Here, we developed a two-stage process consisting of a BES using low-cost and low-reactive carbon sheets as the cathode and anode, and a fixed film reactor (FFR) containing conductive material, i.e., carbon fiber textiles (CFTs) (:BES → FFR). By controlling the cathodic current at 2.7 μA/cm 2 without abiotic H 2 production, the three-electrode BES system was operated to mimic a microbial electrolysis cell.

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The Atrium (University of Guelph) • 2016

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