Hanhan Xue, Yongsen Shi, Junpeng Qiao et al.

Sustainability • 2023

The remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soils has received much attention in recent years, and most of the contaminated sites are in anaerobic environments, such as deep soils and flooded soils. We simulated the natural flooded soil environment, selected phenanthrene (PHE) as a model PAH contaminant, and designed batch experiments run for 63 days to comprehensively investigate the effects of the combined addition of anaerobic sludge and granular biochar on microbial community and function and the anaerobic biodegradation of PHE. Firstly, the residue, distribution, and removal of PHE in the flooded soil environment were quantified for each group. Secondly, the effects of bioaugmentation of soil indigenous microorganisms by the addition of anaerobic activated sludge and biostimulation of biochar on the removal of PHE from the soil were analyzed against each other. Lastly, the changes in the structure of the microbial community under the effect of bioaugmentation and biostimulation were illustrated by sequencing analyses. The results of this study showed that the removal efficiency of PHE reached 72.0% after the addition of anaerobic activated sludge. The incorporation of anaerobic activated sludge and biochar resulted in a 25.3% increase in PHE removal compared to a single soil, suggesting that the combination of bioaugmentation and biostimulation can have a synergistic effect on the anaerobic biodegradation of PHE in contaminated soils. The results of sequencing analysis further indicated that the introduction of an exogenous microbial community changed the dominant genera associated with PHE degradation and introduced methanogenic archaea, which enriched the metabolic pathways of the carbon cycle in the system. On this basis, the addition of biochar resulted in higher anaerobic microbial community diversity, functional dominant species were enriched, and the direct interspecies electron transfer (DIET) process between electroactive bacteria (Bacteroides, f_Geobacteraceae) and Methanosaeta was facilitated, which accelerated the degradation of PHE by anaerobic microbial communities. The results of this study provide regulatory tools and basic data support for enhanced bioremediation of PAHs in flooded soils.

Huan Wang, Yue Zheng, Jiawei Liu et al.

Biosensors and Bioelectronics • 2022

Shiqi Zhang, Yongliang Wu, Lean Zhou et al.

Chemical Engineering Journal • 2024

Sameen Yousaf, Maira Anam, Samia Saeed et al.

Environmental Technology Reviews • 2017

Recent concerns to maximize the power output and achieve the scale-up applications of microbial fuel cell (MFC) technology demand the selection of enriched electroactive consortia from diverse natural habitats such as soil, sediments and wastewater. To further improve the MFC performance, a fundamental knowledge of microbiology of electrochemically active bacteria (EAB) is required. This review presents the various enrichment strategies, engineering and microbiological approaches to optimize the microbial communities with improved electrigenic potential that could be used to enhance the MFC performances. The development of functionally stable electrigenic and electrotrophic microbial communities on anodes and cathodes of MFCs could extend the scope of this technology beyond the bioelectricity generation and wastewater treatment to the electrosynthesis of higher value products and bioremediation. Moreover, investigating the effect of various exogenous factors and limitations on the enrichment of EAB could advance the commercial applications of MFCs.

Liping Liang, Fenfen Xi, Weishou Tan et al.

Biochar • 2021

Abstract Biochar (BC) has exhibited a great potential to remove water contaminants due to its wide availability of raw materials, high surface area, developed pore structure, and low cost. However, the application of BC for water remediation has many limitations. Driven by the intense desire of overcoming unfavorable factors, a growing number of researchers have carried out to produce BC-based composite materials, which not only improved the physicochemical properties of BC, but also obtained a new composite material which combined the advantages of BC and other materials. This article reviewed previous researches on BC and BC-based composite materials, and discussed in terms of the preparation methods, the physicochemical properties, the performance of contaminant removal, and underlying adsorption mechanisms. Then the recent research progress in the removal of inorganic and organic contaminants by BC and BC-based materials was also systematically reviewed. Although BC-based composite materials have shown high performance in inorganic or organic pollutants removal, the potential risks (such as stability and biological toxicity) still need to be noticed and further study. At the end of this review, future prospects for the synthesis and application of BC and BC-based materials were proposed. This review will help the new researchers systematically understand the research progress of BC and BC-based composite materials in environmental remediation.

Yue Liu, Nannan Zhao, Shuo Dai et al.

Journal of Hazardous Materials • 2024

Yinxiu Liang, Min Ji, Hongyan Zhai et al.

The Science of The Total Environment • 2021

Yuan Li, Kai Liu, Rongrong Mao et al.

Journal of Hazardous Materials • 2022

Carolina Cruz Viggi, Matteo Tucci, Marco Resitano et al.

Bioengineering • 2023

Anaerobic bioremediation is a relevant process in the management of sites contaminated by petroleum hydrocarbons. Recently, interspecies electron transfer processes mediated by conductive minerals or particles have been proposed as mechanisms through which microbial species within a community share reducing equivalents to drive the syntrophic degradation of organic substrates, including hydrocarbons. Here, a microcosm study was set up to investigate the effect of different electrically conductive materials (ECMs) in enhancing the anaerobic biodegradation of hydrocarbons in historically contaminated soil. The results of a comprehensive suite of chemical and microbiological analyses evidenced that supplementing the soil with (5% w / w ) magnetite nanoparticles or biochar particles is an effective strategy to accelerate the removal of selected hydrocarbons. In particular, in microcosms supplemented with ECMs, the removal of total petroleum hydrocarbons was enhanced by up to 50% relative to unamended controls. However, chemical analyses suggested that only a partial bioconversion of contaminants occurred and that longer treatment times would have probably been required to drive the biodegradation process to completion. On the other hand, biomolecular analyses confirmed the presence of several microorganisms and functional genes likely involved in hydrocarbon degradation. Furthermore, the selective enrichment of known electroactive bacteria (i.e., Geobacter and Geothrix ) in microcosms amended with ECMs, clearly pointed to a possible role of DIET (Diet Interspecies Electron Transfer) processes in the observed removal of contaminants.

Lecheng Liu, Guangfei Liu, Jiti Zhou et al.

Environmental Science & Technology • 2021

The fate and transport of bacteria in porous media are essential for bioremediation and water quality control. However, the influence of biological activities like extracellular electron transfer (EET) and swimming motility toward granular media on cell transport remains unknown. Here, electroactive bacteria with higher Fe(III) reduction abilities were found to demonstrate greater retention in ferrihydrite-coated sand. Increasing the concentrations of the electron donor (1-10 mM lactate), shuttle (0-50 μM anthraquinone-2,6-disulfonate), and acceptor (ferrihydrite, MnO 2 , or biochar) under flow conditions significantly reduced Shewanella oneidensis MR-1's mobility through redox-active porous media. The deficiency of EET ability or flagellar motion and inhibition of intracellular proton motive force, all of which are essential for energy taxis, enhanced MR-1's transport. It was proposed that EET could facilitate MR-1 to sense, tactically move toward, and attach on redox-active media surface, eventually improving its retention. Positive linear correlations were established among parameters describing MR-1's energy taxis ability (relative taxis index), cell transport behavior (dispersion coefficient and relative change of effluent percentage), and redox activity of media surface (reduction potential or electron-accepting rate), providing novel insights into the critical impacts of bacterial microscale motility on macroscale cell transport through porous media.

Tejedor-Sanz Sara, P. Fernández-Labrador, Carlos Manchón et al.

Electrochemistry Communications • 2020

Microbial Electrochemical Fluidized Bed Reactors (ME-FBR) represent a new concept for promoting proper bacteria-electrode interaction and eventually efficient biocatalysis in Microbial Electrochemical Technologies (METs). In the current work we demonstrate how a fluidized cathode, a dynamic and discontinuous design of electrode, can be an effective electron donor for electroactive hydrogen-generating and nitrate reducing bacteria. Furthermore, the oxygen produced in the anodic reaction promoted ammonium oxidation to nitrate by nitrifying bacteria thus expanding the environmental applications of the system. By coupling both anodic and cathodic reactions, it was possible to simultaneously achieve nitrification–denitrification within one chamber and without external oxygen addition. Our proof-of-concept revealed the removal of 98% ammonium and ca. 29% of total nitrogen (31 g-N m−3reactor d−1) from an effluent with low organic matter under continuous mode. This study reveals for first time how fluidized beds can be integrated in METs not only as anodes but also as cathodes, broadening the opportunities and applications to bioremediation and bioelectrosynthesis processes.

Xin Li, Shiling Zheng, Yinhao Li et al.

Journal of Hazardous Materials • 2023

Xuliang Zhuang, Shijie Wang, Shanghua Wu

Life • 2024

Microorganisms are key players in the global biogeochemical sulfur cycle. Among them, some have garnered particular attention due to their electrical activity and ability to perform extracellular electron transfer. A growing body of research has highlighted their extensive phylogenetic and metabolic diversity, revealing their crucial roles in ecological processes. In this review, we delve into the electron transfer process between sulfate-reducing bacteria and anaerobic alkane-oxidizing archaea, which facilitates growth within syntrophic communities. Furthermore, we review the phenomenon of long-distance electron transfer and potential extracellular electron transfer in multicellular filamentous sulfur-oxidizing bacteria. These bacteria, with their vast application prospects and ecological significance, play a pivotal role in various ecological processes. Subsequently, we discuss the important role of the pili/cytochrome for electron transfer and presented cutting-edge approaches for exploring and studying electroactive microorganisms. This review provides a comprehensive overview of electroactive microorganisms participating in the biogeochemical sulfur cycle. By examining their electron transfer mechanisms, and the potential ecological and applied implications, we offer novel insights into microbial sulfur metabolism, thereby advancing applications in the development of sustainable bioelectronics materials and bioremediation technologies.

Yang Bai, Bin Liang, Hui Yun et al.

Journal of Hazardous Materials • 2020

Haloaromatic antimicrobial triclocarban (TCC) is an emerging refractory contaminant that commonly coexisted with conventional contaminants such as polycyclic aromatic hydrocarbons (PAHs). TCC may negatively affect the metabolic activity of sediment microorganisms and persist in environment; however, remediation methods that relieve the TCC inhibitory effect in sediments remain unknown. Here, a novel electro-biostimulation and bioaugmentation combined remediation system was proposed by the simultaneous introduction of a TCC-degrading Ochrobactrum sp. TCC-2 and electrode into the TCC and PAHs co-contaminated sediments. Results indicated the PAHs and TCC degradation efficiencies of the combined system were 2.9-3.0 and 4.6 times respectively higher than those of the control group (no electro-biostimulation and no bioaugmentation treatments). The introduced strain TCC-2 and the enriched electroactive bacteria and PAHs degraders (e.g. Desulfobulbus, Clostridium, and Paenarthrobacter) synergistically contributed to the accelerated degradation of PAHs and TCC. The preferential elimination of the TCC inhibitory effect through bioaugmentation treatment could restore microbial functions by increasing the functional gene abundances related to various metabolic processes. This study offers new insights into the response of sediment functional communities to TCC stress, electro-biostimulation and bioaugmentation operations and provides a promising system for the enhanced bioremediation of the PAHs and TCC co-contaminated sediments.

Ke Shi, Bin Liang, Qiu Guo et al.

Journal of Hazardous Materials • 2021

Juan Anaya-Garzon, Suanny Mosquera-Romero, Luis Fernando León‐Fernandez et al.

SSRN Electronic Journal • 2022

Debasa Mukherjee, Lucinda Elizabeth Doyle

Electrochimica Acta • 2024

Guowei Chen, Renhao Shen, Yifei Sun et al.

Journal of Water Process Engineering • 2024

Élise Cachat, Weijia Liu, K. Martin et al.

Scientific Reports • 2016

Synthetic biology provides an opportunity for the construction and exploration of alternative solutions to biological problems - solutions different from those chosen by natural life. To this end, synthetic biologists have built new sensory systems, cellular memories, and alternative genetic codes. There is a growing interest in applying synthetic approaches to multicellular systems, especially in relation to multicellular self-organization. Here we describe a synthetic biological system that confers large-scale de novo patterning activity on 2-D and 3-D populations of mammalian cells. Instead of using the reaction-diffusion mechanisms common in real embryos, our system uses cadherin-mediated phase separation, inspired by the known phenomenon of cadherin-based sorting. An engineered self-organizing, large-scale patterning system requiring no prior spatial cue may be a significant step towards the construction of self-assembling synthetic tissues.

Glenn Quek, Ricardo Javier Vázquez, Samantha R. McCuskey et al.

Angewandte Chemie • 2023

Abstract Interfacing bacteria as biocatalysts with an electrode provides the basis for emerging bioelectrochemical systems that enable sustainable energy interconversion between electrical and chemical energy. Electron transfer rates at the abiotic‐biotic interface are, however, often limited by poor electrical contacts and the intrinsically insulating cell membranes. Herein, we report the first example of an n‐type redox‐active conjugated oligoelectrolyte, namely COE‐NDI , which spontaneously intercalates into cell membranes and mimics the function of endogenous transmembrane electron transport proteins. The incorporation of COE‐NDI into Shewanella oneidensis MR‐1 cells amplifies current uptake from the electrode by 4‐fold, resulting in the enhanced bio‐electroreduction of fumarate to succinate. Moreover, COE‐NDI can serve as a “protein prosthetic” to rescue current uptake in non‐electrogenic knockout mutants.

Priyadharshini Mani, T. Keshavarz, T.S. Chandra et al.

Enzyme and Microbial Technology • 2016

Kamran Ayaz, Ewa Zabłocka-Godlewska, Abdullah et al.

Journal of environmental chemical engineering • 2026

Enhancing bioelectrochemical performance through electrode modifications is critical for advancing microbial fuel cell (MFC) technology. This study investigates the effect of carbon cloth modifications using polyaniline (PANI), glucose, and gelatin on biofilm formation, charge transfer, and microbial viability. Cyclic voltammetry results of initial and 7-day performance demonstrate that PANI-glucose-gelatin-biofilm modified carbon cloth (MCC.B) exhibits highest maximum current and lowest charge transfer resistance when compared with a bare carbon cloth-biofilm system with (CC.G.B) and without (CC.B) the presence of dissolved glucose. Biofilm viability studies reveal that CC.G.B supports predominantly planktonic growth, limiting bacterial adhesion, while MCC.B achieves the highest biofilm formation (90.2 ± 0.2%) and charge storage capacity (174.9 ± 10.2 mC/cm 2 ), corresponding to an approximately 50-fold increase in CSC compared with a bare carbon cloth system (3.3 ± 0.3 mC/cm 2 ), highlighting the synergistic impact of surface modifications and microbial activity. These findings demonstrate that MCC.B facilitates extracellular electron transfer by optimizing bacterial adhesion and nutrient availability, making it a promising candidate for shortening start-up and improving early-stage bioelectrode performance, while longer-term stability remains to be established. This work provides new insights into electrode engineering strategies for maximizing microbial interaction and electrochemical performance in next-generation microbial electrochemical technologies. • Novel electrode modification strategy for microbial fuel cells was developed. • Carbon cloth was modified with polyaniline, glucose, and a protective gelatin layer. • Higher biofilm adhesion and improved cell viability were observed on modified electrodes. • Higher capacitance and lower charge transfer resistance were noted with modified carbon cloth.

Dinesh Gupta, Molly C. Sutherland, Karthikeyan Rengasamy et al.

mBio • 2019

Photoferrotrophy is a form of anoxygenic photosynthesis whereby bacteria utilize soluble or insoluble forms of ferrous iron as an electron donor to fix carbon dioxide using light energy. They can also use poised electrodes as their electron donor via phototrophic extracellular electron uptake (phototrophic EEU). The electron uptake mechanisms underlying these processes are not well understood. Using Rhodopseudomonas palustris TIE-1 as a model, we show that a single periplasmic decaheme cytochrome c , PioA, and an outer membrane porin, PioB, form a complex allowing extracellular electron uptake across the outer membrane from both soluble iron and poised electrodes. We observe that PioA undergoes postsecretory proteolysis of its N terminus to produce a shorter heme-attached PioA (holo-PioA C , where PioA C represents the C terminus of PioA), which can exist both freely in the periplasm and in a complex with PioB. The extended N-terminal peptide controls heme attachment, and its processing is required to produce wild-type levels of holo-PioA C and holo-PioA C B complex. It is also conserved in PioA homologs from other phototrophs. The presence of PioAB in these organisms correlate with their ability to perform photoferrotrophy and phototrophic EEU. IMPORTANCE Some anoxygenic phototrophs use soluble iron, insoluble iron minerals (such as rust), or their proxies (poised electrodes) as electron donors for photosynthesis. However, the underlying electron uptake mechanisms are not well established. Here, we show that these phototrophs use a protein complex made of an outer membrane porin and a periplasmic decaheme cytochrome (electron transfer protein) to harvest electrons from both soluble iron and poised electrodes. This complex has two unique characteristics: (i) it lacks an extracellular cytochrome c , and (ii) the periplasmic decaheme cytochrome c undergoes proteolytic cleavage to produce a functional electron transfer protein. These characteristics are conserved in phototrophs harboring homologous proteins.

Yang Li, Han‐Qing Yu, Qiang Tang

Environmental Research • 2025

Junqi Zhang, Wenjing Lv, Yangyang Wang et al.

Chemical Engineering Journal • 2025

Suwon Kim, Ye Sun Han, Gaeun Lim et al.

Bioelectrochemistry • 2025

Namita P. Shroff, Shuai Xu, Julyana Acevedo et al.

Journal of The Electrochemical Society • 2017

Shewanella oneidensis MR-1 employs various methods of Extracellular Electron Transport (EET) to insoluble terminal electron acceptors, including graphite felt anodes of BioElectrochemical Systems (BESs). Fibers in the felt form a three dimensional meshwork within which microbes can form biofilms, as well as occupy the interstitial spaces as planktonic cells. Our results indicate that these interstices generated by the meshwork create a novel microenvironment where planktonic cells grow to higher density under certain conditions. When incubated anaerobically with 18 mM lactate and 30 mM fumarate, planktonic cell counts within the electrodes are ∼10-fold higher than bulk planktonic cell counts; a phenomenon we termed the "interstitial felt effect". Upon lowering both lactate and fumarate concentrations 10-fold, while bulk planktonic cell counts are stable, the interstitial felt effect disappears. This effect reappears when lactate and fumarate concentrations are lowered another 10-fold. Cyclic voltammetry experiments did not reveal any modification of the graphite fibers within the electrodes. A mutant strain lacking the primary flavin transporter gene, bfe, also expresses the interstitial felt effect. The interstitial planktonic microenvironment of electrically inert polyurethane sponge did not demonstrate this phenomenon. This study provides new insights into interactions of microbes with electrode materials that may help improve overall BES performance.

Keisuke Tomita, Atsumi Hirose, Yugo Tanaka et al.

Journal of Bioscience and Bioengineering • 2023

Edina Klein, Hannah Heintz, René Wurst et al.

Scientific Reports • 2024

Biofilm formation by Shewanella oneidensis has been extensively studied under oxic conditions; however, relatively little is known about biofilm formation under anoxic conditions and how biofilm architecture and composition can positively influence current generation in bioelectrochemical systems. In this study, we utilized a recently developed microfluidic biofilm analysis setup with automated 3D imaging to investigate the effects of extracellular electron acceptors and synthetic modifications to the extracellular polymeric matrix on biofilm formation. Our results with the wild type strain demonstrate robust biofilm formation even under anoxic conditions when fumarate is used as the electron acceptor. However, this pattern shifts when a graphite electrode is employed as the electron acceptor, resulting in biofilm formation falling below the detection limit of the optical coherence tomography imaging system. To manipulate biofilm formation, we aimed to express BpfG with a single amino acid substitution in the catalytic center (C116S) and to overexpress bpfA. Our analyses indicate that, under oxic conditions, overarching mechanisms predominantly influence biofilm development, rather than the specific mutations we investigated. Under anoxic conditions, the bpfG mutation led to a quantitative increase in biofilm formation, but both strains exhibited significant qualitative changes in biofilm architecture compared to the controls. When an anode was used as the sole electron acceptor, both the bpfA and bpfG mutations positively impacted mean current density, yielding a 1.8-fold increase for each mutation.

Lukas Kneuer, René Wurst, Johannes Gescher

Advances in biochemical engineering, biotechnology • 2024

Enze Zhou, Feng Li, Dawei Zhang et al.

Water Research • 2022

Megan C Gruenberg, Michaela A. TerAvest

Journal of Industrial Microbiology & Biotechnology • 2023

Shewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances the growth of wild-type S. oneidensis MR-1 on the sugar substrate N-acetylglucosamine (NAG). IPTG improves the carrying capacity of S. oneidensis growing on NAG while the growth rate remains similar to cultures without the inducer. Extracellular acetate accumulates faster and to a higher concentration in cultures without IPTG than those with it. IPTG appears to improve acetate metabolism, which combats the negative effect that acetate accumulation has on the growth of S. oneidensis with NAG. We recommend using extensive experimental controls and careful data interpretation when using both NAG and IPTG in S. oneidensis cultures.

Xiaoyu Yong, Yong-Qi Jiang, Chao Wang et al.

Journal of Environmental Management • 2024

Xinqi Guo, Chenhui Yang, Jiaqi Wu et al.

Journal of Power Sources • 2024

Huixing Wu, Lingyan Ding, Han Wang et al.

Chemical Engineering Journal • 2024

Oluwadamilola Oluwatoyin Hazzan, Collins Chimezie Elendu, Claude Kiki et al.

Biochemical Engineering Journal • 2024

Daobo Li, Xiaodan Zheng, Yonggang Yang et al.

ChemElectroChem • 2021

Abstract Natural microbes employing c ‐type cytochromes ( c ‐Cyts) for extracellular electron transport (EET) were greatly valuable to develop redox‐based bioelectrochemical applications. However, low levels of c ‐Cyt expression limited the phylogenetically diverse Gram‐positive species to be used in bioelectrochemical devices. This work reported the remarkable finding that a high level of c ‐Cyts was expressed in the cells of the Gram‐positive strain Lysinibacillus varians GY32. c ‐Cyts in Lysinibacillus varians GY32 cells were expressed at a level close to those in Shewanella oneidensis . These c ‐Cyts were found to cluster in cytoplasmic membrane and periplasmic space, along the length of Lysinibacillus varians GY32 cell. With voltammetry and spectroelectrochemical titration, phenazine methosulfate‐mediated electron transfer between the c ‐Cyts and an electrode was proved. These results expanded the accessible natural models of EET and suggested a new microbial platform for developing bioelectrochemical applications.

David Rehnlund, Guiyeoul Lim, Laura-Alina Philipp et al.

iScience • 2022

Extracellular electron transfer (EET) from microorganisms to inorganic electrodes is a unique ability of electrochemically active bacteria. Despite rigorous genetic and biochemical screening of the c -type cytochromes that make up the EET network, the individual electron transfer steps over the cell membrane remain mostly unresolved. As such, attempts to transplant entire EET chains from native into non-native exoelectrogens have resulted in inferior electron transfer rates. In this study we investigate how nanostructured electrodes can interface with Shewanella oneidensis to establish an alternative EET pathway. Improved biocompatibility was observed for densely packed nanostructured surfaces with a low cell-nanowire load distribution during applied external forces. External gravitational forces were needed to establish a bioelectrochemical cell-nanorod interface. Bioelectrochemical analysis showed evidence of nanorod penetration beyond the outer cell membrane of a deletion mutant lacking all outer membrane cytochrome encoding genes that was only electroactive on a nanostructured surface and under external force.

Ricardo Javier Vázquez, Samantha R. McCuskey, Glenn Quek et al.

Macromolecular Rapid Communications • 2022

Successful practical implementation of bioelectrochemical systems (BES) requires developing affordable electrode structures that promote efficient electrical communication with microbes. Recent efforts have centered on immobilizing bacteria with organic semiconducting polymers on electrodes via electrochemical methods. This approach creates a fixed biocomposite that takes advantage of the increased electrode's electroactive surface area (EASA). Here, it is demonstrated that a biocomposite comprising the water-soluble conjugated polyelectrolyte CPE-K and electrogenic Shewanella oneidensis MR-1 can self-assemble with carbon paper electrodes, thereby increasing its biocurrent extraction by ≈6-fold over control biofilms. A ≈1.5-fold increment in biocurrent extraction is obtained for the biocomposite on carbon paper relative to the biocurrent extracted from gold-coated counterparts. Electrochemical characterization revealed that the biocomposite stabilized with the carbon paper more quickly than atop flat gold electrodes. Cross-sectional images show that the biocomposite infiltrates inhomogeneously into the porous carbon structure. Despite an incomplete penetration, the biocomposite can take advantage of the large EASA of the electrode via long-range electron transport. These results show that previous success on gold electrode platforms can be improved when using more commercially viable and easily manipulated electrode materials.

Mohammad Kalantar, Mohammad Mahdi Mardanpour, Soheila Yaghmaei

Bioelectrochemistry • 2018