Joseph A. Kerrigan, David Probst, Koji Sode

Biosensors and Bioelectronics • 2026

Dayananda Desagani, Aakash Jog, Orian Teig-Sussholz et al.

Sensors and Actuators B Chemical • 2022

Haixia Wang, Xuejiao Wang, Mingyuan Zhao et al.

Analytical Letters • 2021

Ratiometric signal detection has been widely used in bioelectrochemical sensors because of the good stability and selectivity. A novel biosensor is reported for nucleic acid detection based on the ratio of the oxidation peak current ratio between ferrocene (Fc) and methylene blue (MB) (IFc/IMB) using a proximity-dependent surface hybridization strategy. The design of hairpin capture was shown to reduce the possibility of the hybridization with the detection probe in the absence of the target which improved the specificity. Moreover, this approach improved the signal stability. Additionally, a linear relationship was observed between the ratio of IFc/IMB and the logarithm of the absolute value of DNA concentration from 1.0 × 10−12 M to 1.0 × 10−16 M. This biosensor possessed high sensitivity with a detection limit as low as 0.64 × 10−16 M. Moreover, good selectivity was obtained for the biosensor by identifying the target of the single-base mismatch. Therefore, this ratiometric electrochemical biosensor has great potential applications for nucleic acid detection and medical clinical diagnosis.

Feifei Guo, Tong Li, Xi-Ping Mu et al.

Analytical and Bioanalytical Chemistry • 2023

Shuo Li, Yannick Coffinier, Chann Lagadec et al.

ACS Sensors • 2023

Despite several demonstrations of electrochemical devices with limits of detection (LOD) of 1 cell/mL, the implementation of single-cell bioelectrochemical sensor arrays has remained elusive due to the challenges of scaling up. In this study, we show that the recently introduced nanopillar array technology combined with redox-labeled aptamers targeting epithelial cell adhesion molecule (EpCAM) is perfectly suited for such implementation. Combining nanopillar arrays with microwells determined for single cell trapping directly on the sensor surface, single target cells are successfully detected and analyzed. This first implementation of a single-cell electrochemical aptasensor array, based on Brownian-fluctuating redox species, opens new opportunities for large-scale implementation and statistical analysis of early cancer diagnosis and cancer therapy in clinical settings.

Shuting Jiang, Qinli Pu, Weiyan Zhu et al.

Journal of Nanoelectronics and Optoelectronics • 2020

Based on graphene sheet (GS) and gold-magnetic nanoparticles (Au–Fe 3 O 4 ), a novel bioelectrochemical sensor was developed to detect toxoplasmosis-specific IgM antibody (Tg-IgM). This kind of bioelectrochemical sensor combined the high specific surface area of GS and the high biocompatibility of Au–Fe 3 O 4 nanomaterials. The anti-IgM-HRP modified by Au–Fe 3 O 4 nanoparticles was used as the detection probe, and the preparation method of composite detection probe and the corresponding sensor performance were provided. In addition, by a systematic simulation modeling, this study further investigated the effect of CV scanning speed, pH value, H 2 O 2 concentration and graphene concentration on the detection sensitivity of the proposed bioelectrochemical sensor.

Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Current Opinion in Electrochemistry • 2017

Carla Gimkiewicz, Steffi Hunger, Falk Harnisch

ChemElectroChem • 2016

Abstract Primary microbial electrochemical technologies are utilizing the interfacing of microbial redox reactions and electrodes. Based on its exploitation as recognition element for bioelectrochemical glucose sensors and inspired by its biotechnological potential Gluconobacter oxydans was studied on its suitability for microbial electrosynthesis. It is demonstrated that in bioelectroreactors the conversion of glucose by G. oxydans based on mediated extracellular electron transfer is not related to electric current flow. Oxidation current can only be recorded after preceding aeration phase, but the response does strongly depend on the microbial activity status, p O 2 and gas supply as well as the pH regime. It is proven that the electric current is derived from the microbial cells, but the mechanism still needs to be elucidated. The suitability of G. oxydans for microbial electrosynthesis but also for bioelectrochemical sensors is critically assessed.

Lijuan Zhang, Sam Fong Yau Li, Hu‐Chun Tao

Environmental Geochemistry and Health • 2018

Simon Grall, Shuo Li, Laurent Jalabert et al.

Physical Review Letters • 2023

Redox monolayers are the base for a wide variety of devices including high-frequency molecular diodes or biomolecular sensors. We introduce a formalism to describe the electrochemical shot noise of such a monolayer, confirmed experimentally at room temperature in liquid. The proposed method, carried out at equilibrium, avoids parasitic capacitance, increases the sensitivity, and allows us to obtain quantitative information such as the electronic coupling (or standard electron transfer rates), its dispersion, and the number of molecules. Unlike in solid-state physics, the homogeneity in energy levels and transfer rates in the monolayer yields a Lorentzian spectrum. This first step for shot noise studies in molecular electrochemical systems opens perspectives for quantum transport studies in a liquid environment at room temperature as well as highly sensitive measurements for bioelectrochemical sensors.

Bo Song, Qi Wang, Jafar Ali et al.

Chemical Engineering Journal • 2023

Kayode Olaifa, Jasmina Nikodinović‐Runić, Biljana Đ. Glišić et al.

Electrochimica Acta • 2021

Shuo Li, Yannick Coffinier, Chann Lagadec et al.

Biosensors and Bioelectronics • 2022

Drochss P. Valencia, Luiza Maria Ferreira Dantas, Alexandre Lara et al.

Journal of Electroanalytical Chemistry • 2016

Hui Xie, Xiaoyan Li, Guiling Luo et al.

Diamond and Related Materials • 2019

Tian Li, Xin Wang, Qixing Zhou et al.

ACS Sensors • 2018

Acid rain poses significant threats to crops and causes a decline in food production, but current monitoring and response to acid rain damage is either slow or expensive. The direct damage observation on plants can take several hours to days when the damage is irreversible. This study presents a real time bioelectrochemical monitoring approach that can detect acid rain damage within minutes. The rhizospheric bioelectrochemical sensor (RBS) takes advantage of the fast chain responses from leaves to roots, and then to the microbial electrochemical reactions in the rhizosphere. Immediate and repeatable current fluctuations were observed within 2 min after acid rain, and such changes were found to correspond well to the changes in rhizospheric organic concentration and electrochemical responses. Such correlation not only can be observed during acid rain events that can be remedied via rinsing, but it was also validated when such damage is irreversible, resulted in zero current, photosynthetic efficiency, and electrochemical signals. The alanine, aspartate, and glutamate metabolism and galactose metabolism in leaves and roots were inhibited by the acid rain, which resulted in the decrease of rhizodeposits such as fumaric acid, d-galactose, and d-glucose. These changes resulted in reduced electroactivity of anodic microorganisms, which was confirmed by a reduced redox current, a narrower spectrum in differential pulse voltammetry, and the loss of peak in the Bode plot. These findings indicate that the RBS process can be a simple, swift, and low-cost monitoring tool for acid rain that allows swift remediation measures, and its potential may be broadened to other environmental monitoring applications.

Jon Chouler, Matthew D. Monti, William J. Morgan et al.

Electrochimica Acta • 2019

Effective detection of contaminants in water is the basis of safe water provision to communities. Traditional analytical methods are, however, expensive, time-consuming and cannot be easily adapted to emerging pollutants (i.e. herbicides, pharmaceuticals and their metabolites). Microalgae have been shown to be an ideal sensing probe for bioactive compounds, with great sensitivity and limit of detections at very low concentration levels (nM range). In this study, we explore the use of microalgae in microbial fuel cells (MFCs) as a means to generate a sensitive, portable and cost-effective bioelectrochemical sensor for onsite monitoring of pollutants in water. In particular, we report an innovative miniature single chamber photosynthetic MFC (photoMFC) and demonstrate its ability to detect formaldehyde, a highly toxic compound that can arise in drinking water from the oxidation of natural organic matter during ozonation and chlorination. The photoMFC, inoculated with a mixed microalgae culture from a wastewater treatment algal pond, generated a peak power and current density of 0.18 mW m−2 and 7.2 mA m−2 respectively, when exposed to light. A current response to formaldehyde proportional to its concentration was produced in less than 1 h, with a sensitivity of 69.2 ± 16.7%−1 cm−2. As such, this work provides the first miniature photosynthetic MFC device water shock sensor, with the great benefit of simple operation (with light being the sole energy source) and rapid onsite biosensing capability.

Matteo Tucci, Paolo Bombelli, Christopher J. Howe et al.

Microorganisms • 2019

A novel mediatorless photo-bioelectrochemical sensor operated with a biofilm of the cyanobacterium Synechocyst i s PCC6803 wt . for herbicide detection with long term stability (>20 days) was successfully developed and tested. Photoanodic current generation was obtained in the absence of artificial mediators. The inhibitory effect on photocurrent of three commonly used herbicides (i.e., atrazine, diuron, and paraquat) was used as a means of measuring their concentrations in aqueous solution. The injection of atrazine and diuron into the algal medium caused an immediate photocurrent drop due to the inhibition of photosynthetic electron transport. The detected concentrations were suitable for environmental analysis, as revealed by a comparison with the freshwater quality benchmarks set by the Environmental Protection Agency of the United States (US EPA). In contrast, paraquat caused an initial increase (~2 h) of the photocurrent effect of about 200%, as this compound can act as a redox mediator between the cells and the anode. A relatively long-term stability of the biosensor was demonstrated, by keeping anodes colonized with cyanobacterial biofilm in the dark at 4 °C. After 22 days of storage, the performance in terms of the photocurrent was comparable with the freshly prepared biosensor. This result was confirmed by the measurement of chlorophyll content, which demonstrated preservation of the cyanobacterial biofilm. The capacity of this biosensor to recover after a cold season or other prolonged environmental stresses could be a key advantage in field applications, such as in water bodies and agriculture. This study is a step forward in the biotechnological development and implementation of storable mediatorless electrochemical biosensors for herbicide detection.

Solange E. Astorga, Liangxing Hu, Enrico Marsili et al.

Materials & Design • 2019

Extracellular electron transfer (EET) from microorganisms to insoluble metals and electrodes is relevant to energy recovery from wastewater, green production of high-added value chemicals, and biosensors for food, environmental, and clinical applications. Microstructured electrode surfaces increase EET rate in bioelectrochemical systems, thus enabling higher sensibility and power output as well as the detection of bacteria and biofilms in bioelectrochemical sensors. However, many aspects of the EET process, particularly in early biofilm stages, are still poorly understood. We report a microstructured gold electrode maintained at oxidative potential to support the growth of Escherichia coli, measure the electrochemical output, and analyze the EET rate during early biofilm formation. The charge outputs of the modified electrodes are up to 22% higher than the control electrodes, enabling the electrochemical detection of early E. coli biofilms. The electrode microstructures promote biofilm attachment, as confirmed by field emission scanning electron microscope (FESEM) and confocal laser scanning microscope (CLSM) imaging. Following biofilm formation, the resistance to charge transfer at the biofilm-electrode interface decreases and the capacitance increases as shown by EIS analysis. Overall, these results contribute to the understanding of EET in early biofilms, towards developing sensitive bioelectrochemical sensors for biofilm detection.

Hui Shu, Tingrun Lai, Zhichao Yang et al.

Food Chemistry • 2022

Tian Li, Chengmei Liao, Jingkun An et al.

The Science of The Total Environment • 2020

Joshua van der Zalm, Shuai Chen, Wei Huang et al.

Journal of The Electrochemical Society • 2020

In the fields of medicine, environmental protection, and food safety, sensors are imperative for the detection of biomarkers, contaminants, and preservatives. The use of nanoporous gold (NPG) as a sensing platform may greatly enhance performance due to its stability, high surface area, and catalytic abilities. There are many methods reported in the literature for fabricating NPG, including chemical strategies and various electrochemical techniques. The primarily use of NPG in sensing applications may be classified into three categories: electrochemical, bioelectrochemical, and optical. Although both electrochemical and bioelectrochemical sensors are based on the electrical signal produced by a specific analyte, a biological recognition element is involved in the bioelectrochemical sensing process. On the other hand, optical sensors exploit NPG through unique surface plasmon resonance properties that can be monitored by UV-Vis, Raman, or fluorescence spectroscopy. For this review, the primary strategies for fabricating NPG, including dealloying, electrochemical, and dynamic hydrogen bubble template (DHBT), are discussed. In addition, advances made over the last decade towards the detection of biomarkers, pollutants, contaminants, and food additives are highlighted. The future development of NPG based sensors for medical, environmental, and food safety applications is discussed.

Mohammed Sedki, Rabeay Y. A. Hassan, Amr Hefnawy et al.

Sensors and Actuators B Chemical • 2017

Melinda David, Adrian Şerban, Cristiana Rădulescu et al.

Bioelectrochemistry • 2019

Chao-Chin Chang, Shiue-Lin Li, Zhong-Xian Wu et al.

Biosensors and Bioelectronics • 2023

Hang Li, ChengGang Wu, Xi Wang et al.

Chemical Papers • 2022

Yanping Zhao, Xu Geng, Xiaoling Zhou et al.

Analytica Chimica Acta • 2023

Kartik Aiyer, Debasa Mukherjee, Lucinda Elizabeth Doyle

ACS Applied Bio Materials • 2023

Electroactive microorganisms are now understood to be abundant across nature, though many are categorized as "weak electricigens" not suitable for reasonable power generation. We report the use of weak electricigens from the natural environment for rapid, real-time water quality monitoring. Using a variety of pesticides as model chemical pollutants, the bioelectrochemical sensor was responsive within minutes at all concentrations tested (0.05-2 ppm) and could be repreatedly used long-term. Due to the prevalence of electroactive microorganisms in the natural environment, such sensors could work in tandem with conventional monitoring methods and may be useful for detecting emerging contaminants.

Martin W. A. Spurr, Eileen Hao Yu, Keith Scott et al.

Biosensors and Bioelectronics • 2021

Microbial Fuel Cells (MFCs) operated as biosensors could potentially enable truly low-cost, real-time monitoring of organic loading in wastewaters. The current generated by MFCs has been correlated with conventional measures of organic load such as Biochemical Oxygen Demand (BOD), but much remains to be established in terms of the reliability and applicability of such sensors. In this study, batch-mode and multi-stage, flow-mode MFCs were operated for over 800 days and regularly re-calibrated with synthetic wastewater containing glucose and glutamic acid (GGA). BOD 5 calibration curves were obtained by normalising the current measured as a percentage of maximum current. There was little drift between recalibrations and non-linear Hill models of the combined dataset had R 2 of 88-95%, exhibiting a stable response over time and across devices. Nonetheless, factors which do affect calibration were also assessed. Increasing external resistance (from 43.5 to 5100 Ω) above the internal resistance determined by polarisation curve decreased the calibration upper limit from 240 to 30 mg/l O 2 BOD 5 . Furthermore, more fermentable carbon sources increased the detection range, as tested with samples of real wastewater and synthetic media containing GGA, glucose-only and glutamic acid-only. Biofilm acclimatisation therefore did not account for differences between aerobic oxygen demand determinations and anaerobic MFC responses; these are likely attributable to competitive processes such as fermentation. This further highlights the potential for MFCs as real-time sensors for organic load monitoring and process control in addition to BOD-compliant measurement systems.

Palanisamy Kannan, Prasanna Jogdeo, Abeed Fatima Mohidin et al.

Electrochimica Acta • 2019

Sonia Bahrani, Zahra Razmi, Mehrorang Ghaedi et al.

Ultrasonics Sonochemistry • 2017

Qianbin Wang, Shuibo Liu, Li‐Yun Fu et al.

Analytica Chimica Acta • 2018

Hao Sun, Mingyi Xu, Shubiao Wu et al.

Chemosphere • 2021

Xiang Qi, Panpan Liu, Peng Liang et al.

The Science of The Total Environment • 2020

Hao Sun, İrini Angelidaki, Shubiao Wu et al.

Frontiers in Microbiology • 2019

Acetate as the dominant fraction of volatile fatty acids (VFAs) is an important intermediate in metabolic pathways of methanogenesis, which could reflect the stability status of anaerobic digestion (AD) process. Bioelectrochemical sensors for environmental or bioprocess monitoring have become increasingly attractive in recent years. Although it was more favorable, several challenges still need to be addressed for acetate detection, including large electrode spacing, low stability, biofouling at the cathode and low detection range. In this study, an innovative biosensor on the basis of a three-chamber microbial electrochemical system was proposed to monitor the acetate during the AD process. In such a system, acetate was first transferred from sample chamber through the anion exchange membrane (AEM) to anode due to the driven force of concentration difference and then oxidized by anodic biofilm as a substrate for the current generation. With such design, the influence of waste properties fluctuation in the cathodic reaction could be avoided. The response of current density to different acetate concentrations was investigated. The selectivity, the influence of the sample temperature and the external resistance were also evaluated. The correlation ( R 2 > 0.99) between the current densities and acetate concentrations (up to 160 mM) was established at specific reaction time (from 2 to 5 h). Current densities after 5 h reaction were improving about 20% when the sample temperature was high (e.g., 37 and 55°C). The detection range increased along with the decrease of external resistance. The acetate concentrations of AD effluents as determined by the biosensor where within 24.2% of the ones determined by gas chromatography. Nevertheless, the application of the biosensor for monitoring acetate in environmental samples could still be promising.

Weifeng Liu, Zihao Zhou, Lin Yin et al.

Sensors and Actuators B Chemical • 2018

Tian Li, Xin Wang, Lean Zhou et al.

ACS Sensors • 2016

Flocculants have been used to clarify water for thousands of years. However, the in situ evaluation of flocculant toxicity is difficult because flocculants usually exist as growing complex flocs which is hard to monitor. With alum (KAl(SO4)2·12H2O) as the typical flocculant, a bioelectrochemical sensor is designed to in situ detect its bacterial toxicity. The attenuation ratio of current densities linearly increased with alum concentration (R2 > 0.98) with a slope of 0.0054 A m–2 drop per mg L–1 of alum, indicating a typical toxic response of alum. Turnover and nonturnover cyclic voltammetries (CVs) revealed that the alum inhibited the electrochemical activity of bacteria rather than changing the electron transfer pathways. Alum also hindered the diffusion by flocculation at a concentration larger than 100 mg L–1, which was further confirmed by the linear decrease in viability when biofilm thickness increased. It was revealed that both inactivation of biofilm and influences on diffusion by alum can be detected by bioelectrochemical sensors, which provided a new platform to in situ investigate the biological toxicity of new flocculants.

Fabrice Tanguay‐Rioux, Emmanuel Onyekachi Nwanebu, Manish Thadani et al.

Biochemical Engineering Journal • 2023

В. Г. Дебабов

Applied Biochemistry and Microbiology • 2017

Electrobiosynthesis conducted by microorganisms represents a new technology with great potential. This review considers mechanisms of direct electron transfer from cathode to bacterial cell and a number of anaerobic processes catalyzed with such transport: the biosynthesis of hydrogen, methane, and multicarbon compounds. The possibilities for the use of electrolysis hydrogen to grow hydrogen oxidizing bacteria are also considered, as well as some examples of electricity that influence the reductive and oxidative processes occurring during fermentation. Realization of the electric biosynthesis potential would require deep fundamental research on the mechanisms of extracellular electron transport and the coupling of electric and metabolic processes. Work would be required to reorganize microbial genomes to intensify their metabolism and broaden the repertoire of synthesized metabolites. Progress in these technologies would depend not only on improvements in microorganisms but also on the successful creation of effective biocompatible electrodes and the designing of highly productive reactors.

Hui Yao, Johanna M. Rinta‐Kanto, Igor Vassilev et al.

Applied Microbiology and Biotechnology • 2024

Methanol is a promising feedstock for the bio-based economy as it can be derived from organic waste streams or produced electrochemically from CO 2 . Acetate production from CO 2 in microbial electrosynthesis (MES) has been widely studied, while more valuable compounds such as butyrate are currently attracting attention. In this study, methanol was used as a co-substrate with CO 2 to enhance butyrate production in MES. Feeding with CO 2 and methanol resulted in the highest butyrate production rates and titres of 0.36 ± 0.01 g L -1 d -1 and 8.6 ± 0.2 g L -1 , respectively, outperforming reactors with only CO 2 feeding (0.20 ± 0.03 g L -1 d -1 and 5.2 ± 0.1 g L -1 , respectively). Methanol acted as electron donor and as carbon source, both of which contributed ca. 50% of the carbon in the products. Eubacterium was the dominant genus with 52.6 ± 2.5% relative abundance. Thus, we demonstrate attractive route for the use of the C1 substrates, CO 2 and methanol, to produce mainly butyrate. KEY POINTS: • Butyrate was the main product from methanol and CO 2 in MES • Methanol acted as both carbon and electron source in MES • Eubacterium dominating microbial culture was enriched in MES.