Phuc Thi Ha, Stephen R. Lindemann, Liang Shi et al.

Nature Communications • 2017

Microbial phototrophs, key primary producers on Earth, use H 2 O, H 2 , H 2 S and other reduced inorganic compounds as electron donors. Here we describe a form of metabolism linking anoxygenic photosynthesis to anaerobic respiration that we call 'syntrophic anaerobic photosynthesis'. We show that photoautotrophy in the green sulfur bacterium Prosthecochloris aestaurii can be driven by either electrons from a solid electrode or acetate oxidation via direct interspecies electron transfer from a heterotrophic partner bacterium, Geobacter sulfurreducens. Photosynthetic growth of P. aestuarii using reductant provided by either an electrode or syntrophy is robust and light-dependent. In contrast, P. aestuarii does not grow in co-culture with a G. sulfurreducens mutant lacking a trans-outer membrane porin-cytochrome protein complex required for direct intercellular electron transfer. Syntrophic anaerobic photosynthesis is therefore a carbon cycling process that could take place in anoxic environments. This process could be exploited for biotechnological applications, such as waste treatment and bioenergy production, using engineered phototrophic microbial communities.

Shraddha Yadav, Monali Priyadarshini, Azhan Ahmad et al.

Journal of Hazardous Toxic and Radioactive Waste • 2024

Hydrochar (HC), a carbon (C)-rich solid material produced using a hydrothermal technique at relatively low temperatures and pressures, has gained little attention regarding its use as a nanocatalyst in multiple wastewater treatment technologies. These HC-based nanocatalysts, catalyst support materials, and nano-adsorbents have the advantage of being cost-effective and chemically stable, having a porous structure, semiconductive properties, and oxygen (O)-rich surface functionality. Laboratory-scale trials that have successfully used HC catalysts to accelerate the kinetics of advanced oxidation processes (AOPs) (photocatalysis, electrochemical oxidation, Fenton catalysis) are here reviewed in depth, with a special focus on the mechanisms involved. The potential of HC catalysts in energy and valuables recovery using anaerobic digestion, bioelectrochemical systems, metal air batteries, transesterification, isomerization, and reforming processes are highlighted. In addition, the synthesis of HC from waste biomass (a zero-cost C precursor) would reduce the cost of expensive electrocatalysts, reduce greenhouse gas (GHG) emissions, ensure C capture, offer a low-C economy with less of a C footprint, and enhance environmental sustainability. This review provides a unique and comprehensive sustainability related perspective on HC and HC-based nanocatalysts that can be used to positively impact the United Nations’ Sustainable Development Goals (SDGs), especially SDGs 3, 6, 7, 11, and 13. Finally, a circular economy approach that includes HC and HC catalyst use in wastewater treatment and waste management systems is discussed, along with future perspectives and challenges.

Ahmad Rafiee, Kaveh Khalilpour, James Prest et al.

Biomass and Bioenergy • 2020

Nathalie Gontard, Ulf Sonesson, Morten Birkved et al.

Critical Reviews in Environmental Science and Technology • 2018

Agricultural waste is a huge pool of untapped biomass resources that may even represent economic and environmental burdens. They can be converted into bioenergy and bio-based products by cascading conversion processes, within circular economy, and should be considered residual resources. Major challenges are discussed from a transdisciplinary perspective, focused on Europe situation. Environmental and economic consequences of agricultural residue management chains are difficult to assess due to their complexity, seasonality and regionality. Designing multi-criteria decision support tools, applicable at an early-stage of research, is discussed. Improvement of Anaerobic Digestion (AD), one of the most mature conversion technologies, is discussed from a technological point of view and waste feedstock geographical and seasonal variations. Using agricultural residual resources for producing high-value chemicals is a considerable challenge analysed here, taking into account innovative eco-efficient and cost-effective cascading conversion processes (bio-refinery concept). Moreover, the promotion of agricultural residues-based business is discussed through industrial ecology, to promote synergy, on a local basis, between different agricultural and industrial value chains. Finally, to facilitate a holistic approach and optimise materials and knowledge flows management, the connection of stakeholders is discussed to promote cross-sectorial collaboration and resource exchange at appropriate geographic scales.

Jeffrey L. Moran, Jonathan D. Posner

Annual Review of Fluid Mechanics • 2016

It is well-known that micro- and nanoparticles can move by phoretic effects in response to externally imposed gradients of scalar quantities such as chemical concentration or electric potential. A class of active colloids can propel themselves through aqueous media by generating local gradients of concentration and electrical potential via surface reactions. Phoretic active colloids can be controlled using external stimuli and can mimic collective behaviors exhibited by many biological swimmers. Low–Reynolds number physicochemical hydrodynamics imposes unique challenges and constraints that must be understood for the practical potential of active colloids to be realized. Here, we review the rich physics underlying the operation of phoretic active colloids, describe their interactions and collective behaviors, and discuss promising directions for future research.

Linus Onwuemezie

Energy 360. • 2024

Hydrogen ( H 2 ) has a big role to play in energy transition to achieve net-zero carbon emissions by 2050. For H 2 to compete with other fuels in the energy market, more research is required to mitigate key issues like greenhouse gas (GHG) emissions, safety, and end-use costs. For these reasons, a software-supported technical overview of H 2 production, storage, transportation, and utilisation is introduced. Drawbacks and mitigation approaches for H 2 technologies were highlighted. The recommended areas include solar thermal or renewable-powered plasma systems for feedstock preheating and oxy-hydrogen combustion to meet operating temperatures and heat duties due to losses; integration of electrolysis of H 2 O into hydrocarbon reforming methods to replace air separation unit (ASU); use of renewable power sources for electrical units and the introduction of thermoelectric units to maximise the overall efficiency. Furthermore, a battolyser system for small-scale energy storage; new synthetic hydrides with lower absorption and desorption energy; controlled parameters and steam addition to the combustor/cylinder and combustors with fitted heat exchangers to reduce emissions and improve the overall efficiency are also required. This work also provided detailed information on any of these systems implementations based on location factors and established a roadmap for H 2 production and utilisation. The proposed H 2 production technologies are hybrid pyrolysis-electrolysis and integrated AD-MEC and DR systems using renewable, bioelectrochemical and low-carbon energy systems. Production and utilisation of synthetic natural gas (NG) using renewable-powered electrolysis of H 2 O , oxy-hydrogen and direct air capture (DAC) is another proposed H 2 energy system for a sustainable H 2 economy. By providing these factors and information, researchers can work towards pilot development and further efficiency enhancement. • Detailed software-based H 2 production processes are introduced. • Software-aided H 2 storage methods are presented. • Numerical schematics to describe the use of H 2 in fuel cells and heat engines. • Areas for future research and proposed roadmap for H 2 energy systems are established.

Shams Forruque Ahmed, Nazifa Rafa, M. Mofijur et al.

Frontiers in Energy Research • 2021

The commercialization of hydrogen as a fuel faces severe technological, economic, and environmental challenges. As a method to overcome these challenges, microalgal biohydrogen production has become the subject of growing research interest. Microalgal biohydrogen can be produced through different metabolic routes, the economic considerations of which are largely missing from recent reviews. Thus, this review briefly explains the techniques and economics associated with enhancing microalgae-based biohydrogen production. The cost of producing biohydrogen has been estimated to be between $10 GJ -1 and $20 GJ −1 , which is not competitive with gasoline ($0.33 GJ −1 ). Even though direct biophotolysis has a sunlight conversion efficiency of over 80%, its productivity is sensitive to oxygen and sunlight availability. While the electrochemical processes produce the highest biohydrogen (>90%), fermentation and photobiological processes are more environmentally sustainable. Studies have revealed that the cost of producing biohydrogen is quite high, ranging between $2.13 kg −1 and 7.24 kg −1 via direct biophotolysis, $1.42kg −1 through indirect biophotolysis, and between $7.54 kg −1 and 7.61 kg −1 via fermentation. Therefore, low-cost hydrogen production technologies need to be developed to ensure long-term sustainability which requires the optimization of critical experimental parameters, microalgal metabolic engineering, and genetic modification.

Gahyun Baek, Jaai Kim, Jinsu Kim et al.

Energies • 2018

Anaerobic digestion (AD) is an effective biological treatment for stabilizing organic compounds in waste/wastewater and in simultaneously producing biogas. However, it is often limited by the slow reaction rates of different microorganisms’ syntrophic biological metabolisms. Stable and fast interspecies electron transfer (IET) between volatile fatty acid-oxidizing bacteria and hydrogenotrophic methanogens is crucial for efficient methanogenesis. In this syntrophic interaction, electrons are exchanged via redox mediators such as hydrogen and formate. Recently, direct IET (DIET) has been revealed as an important IET route for AD. Microorganisms undergoing DIET form interspecies electrical connections via membrane-associated cytochromes and conductive pili; thus, redox mediators are not required for electron exchange. This indicates that DIET is more thermodynamically favorable than indirect IET. Recent studies have shown that conductive materials (e.g., iron oxides, activated carbon, biochar, and carbon fibers) can mediate direct electrical connections for DIET. Microorganisms attach to conductive materials’ surfaces or vice versa according to particle size, and form conductive biofilms or aggregates. Different conductive materials promote DIET and improve AD performance in digesters treating different feedstocks, potentially suggesting a new approach to enhancing AD performance. This review discusses the role and potential of DIET in methanogenic systems, especially with conductive materials for promoting DIET.

Xing Ma, Xu Wang, Kersten Hahn et al.

ACS Nano • 2016

The quest for biocompatible microswimmers powered by compatible fuel and with full motion control over their self-propulsion is a long-standing challenge in the field of active matter and microrobotics. Here, we present an active hybrid microcapsule motor based on Janus hollow mesoporous silica microparticles powered by the biocatalytic decomposition of urea at physiological concentrations. The directional self-propelled motion lasts longer than 10 min with an average velocity of up to 5 body lengths per second. Additionally, we control the velocity of the micromotor by chemically inhibiting and reactivating the enzymatic activity of urease. The incorporation of magnetic material within the Janus structure provides remote magnetic control on the movement direction. Furthermore, the mesoporous/hollow structure can load both small molecules and larger particles up to hundreds of nanometers, making the hybrid micromotor an active and controllable drug delivery microsystem.

Marika Alida Johanna Zegers, Eva Augustijn, Geurt Jongbloed et al.

Chemical Engineering Journal • 2025

• Lack of replicability in MES remains understudied with limited abiotic data. • Novel miniaturised MES reactors used to investigate (a)biotic replicability issues. • Statistical tools provide insights into correlations in abiotic and biotic MES data. • Electrode kinetics and porosity impact MES biofilm growth and performance. • Novel miniaturised MES reactor represents scalable state-of-the-art reactor design. Carbon capture and utilisation are crucial for reducing fossil fuel dependence and transforming the chemical and energy industries. Microbial electrosynthesis (MES) is a promising technology where electrotrophic microorganisms convert CO 2 into valuable biochemicals using electricity. Despite recent advancements, replicability in MES remains poorly understood, with scarce pre-inoculation abiotic data and limited exploration of abiotic and biotic performance correlations. This study introduces a novel miniaturised reactor, modelled after a state-of-the-art flat-plate directed-flow-through bioelectrochemical reactor (DFBR). Four miniaturised reactors were tested in parallel under abiotic conditions to evaluate the impact of electrode material, reactor design, and assembly on replicability of electrochemical behaviour. Using the dynamic time warping (DTW) algorithm, reactor similarity was quantified for the first time based on electrochemical performance. Kernel scatterplot smoothing on micro-CT data revealed that electrodes, particularly the commonly used carbon felt, are a significant source of variability in electrochemical performance, as further supported by additional abiotic electrochemical tests. Additionally, the miniaturised reactors were inoculated with an enriched mixed culture to examine microbial activity’s effect on replicability, achieving concentrations up to 4.55 g L -1 acetate, 0.96 g L -1 butyrate, and 0.38 g L -1 caproate after 60 days. Variations in abiotic conditions, including maximum reachable current density, onset potential, and porosity, influence biofilm growth and performance. The miniaturised DFBR effectively represents the serpentine DFBR, while the adaptable reactor design and proposed statistical methods set a new benchmark for MES research.

S.H. Light, Lin Su, Rafael Rivera‐Lugo et al.

Nature • 2018

Anne P.M. Velenturf, Sophie Archer, Helena I. Gomes et al.

The Science of The Total Environment • 2019

A circular economy offers solutions for global sustainability challenges through the transition from the linear take-make-use-dispose economy to a better organisation of resources. However, realising a circular economy has ran into various biophysical constraints. Circular economy implementation is shaped by the Ellen MacArthur Foundation's butterfly diagram that depicts 'biological' and 'technical' flows as separate cycles, subsequently interpreted as organic materials circulating in open loop systems via the environment and inorganic materials circulating in closed loop systems within society. Conversely, in our view, resource flows often contain tightly bound combinations of organic and inorganic materials either due to their natural composition or due to their technical design. Building on this observation, a new diagram is proposed that broadens the scope of the circular economy to cover extractive sectors and the return of materials from anthropogenic use to natural reserves, thereby reshaping the conceptual space within which solutions such as effective zero-waste-residue technologies, business models, and policies can be developed for the optimal management of integrated resources from a whole-system perspective. The diagram offers a realistic outlook on the biophysical limitations of circularity and endeavours to inspire discussion that supports the transition towards a sustainable circular economy.

Michael D. Burkart, Nilay Hazari, Cathy L. Tway et al.

ACS Catalysis • 2019

The environmental and societal consequences of the increasing levels of carbon dioxide in our atmosphere are among the most significant challenges society currently faces. Carbon dioxide utilization, in which carbon dioxide is either used directly or converted into more valuable products, is likely to be one component of a broad strategy to reduce carbon dioxide emissions, a challenge that will require both technological and policy changes. Catalysis is crucial to the successful conversion of carbon dioxide into value-added products. Here, we provide a review on chemical and biological systems for carbon dioxide conversion directed toward the readers of ACS Catalysis, which focuses on providing a general perspective on the field, rather than technical details. We discuss both challenges related to the conversion of carbon dioxide into specific products such as carbon monoxide, formic acid, methanol, methane, ethylene, fuels, carboxylic acids, and polymers as well as general challenges for the field. We also compare and contrast different methods for carbon dioxide conversion, for example homogeneous versus heterogeneous catalysis or photosynthetic versus nonphotosynthetic biological conversion, and highlight areas where one approach may have advantages over another. In a concluding section, we identify problems related to carbon dioxide conversion that will need to be addressed for technology to be both viable and reduce carbon dioxide emissions.

Cheng Sun, Qilin Yu, Zhiqiang Zhao et al.

Journal of Cleaner Production • 2024

Thi Thu Ha Nguyen, Dipak A. Jadhav, Tasnim Eisa et al.

Process Safety and Environmental Protection • 2024

Zanyun Ying, Qianlinglin Qiu, Jiexu Ye et al.

Renewable and Sustainable Energy Reviews • 2024

Renju, Rajesh Singh

Journal of Cleaner Production • 2024

Zhongyi Zhang, Xueqin Lü, Chengxin Niu et al.

Fuel • 2021

Angela Sherry, Luiza L. Andrade, Anne P.M. Velenturf et al.

Microbial Biotechnology • 2017

As we transition from fossil fuel reliance to a new energy future, innovative microbial biotechnologies may offer new routes to maximize recovery from conventional and unconventional energy assets; as well as contributing to reduced emission pathways and new technologies for carbon capture and utilization. Here we discuss the role of microbiology in petroleum biotechnologies in relation to addressing UN Sustainable Development Goal 12 (ensure sustainable consumption and production patterns), with a focus on microbially-mediated energy recovery from unconventionals (heavy oil to methane), shale gas and fracking, bioelectrochemical systems for the production of electricity from fossil fuel resources, and innovations in synthetic biology. Furthermore, using wastes to support a more sustainable approach to fossil fuel extraction processes is considered as we undertake the move towards a more circular global economy.

Fanzhen Lin, Wenwei Li, Dan Wang et al.

Frontiers in Bioengineering and Biotechnology • 2024

Succinic acid (SA), one of the 12 top platform chemicals produced from biomass, is a precursor of various high value-added derivatives. Specially, 1 mol CO 2 is assimilated in 1 mol SA biosynthetic route under anaerobic conditions, which helps to achieve carbon reduction goals. In this review, methods for enhanced CO 2 fixation in SA production and utilization of waste biomass for SA production are reviewed. Bioelectrochemical and bioreactor coupling systems constructed with off-gas reutilization to capture CO 2 more efficiently were highlighted. In addition, the techno-economic analysis and carbon sequestration benefits for the synthesis of bio-based SA from CO 2 and waste biomass are analyzed. Finally, a droplet microfluidics-based high-throughput screening technique applied to the future bioproduction of SA is proposed as a promising approach.

Xizi Long, Hui Wang, Chuqiao Wang et al.

Environmental Technology • 2019

The demand for removal of refractory organic pollutants limits the application of microbial fuel cells. In this study, the synergistic effects of bioelectrochemical and photocatalysis methods were captured by constructing a biophoto anode from a combination of WO 3 /TiO 2 and carbon felt. This biophoto electrode was able to decrease the aniline concentration from 63.3 ± 6.2 to 9.3 ± 5.5 mg/L. The structure of the benzene ring was broken through strong oxidation by photocatalysis. Electrochemical analysis showed that photocatalysis also enhanced the extracellular electron transfer of microorganisms and reduced the resistance of the anode from 136.9 Ω to 69.9 Ω. In addition, the maximum current output increased by 28.5% under the composite biophoto electrode. Further analysis of the microbial community indicated that the biophoto electrode promoted the enrichment of Geobacter in the anode. This biophoto electrode provided a method for overcoming the disadvantages of anaerobic degradation of refractory organics.

Tianyu Gao, Hanmin Zhang, Xiaotong Xu et al.

The Science of The Total Environment • 2021

Yuting He, Qing Li, Jun Li et al.

Renewable Energy • 2021

Oleksandr S. Bushuyev, Phil De Luna, Cao‐Thang Dinh et al.

Joule • 2018

P.R. Yaashikaa, A. Saravanan, P. Senthil Kumar et al.

International Journal of Hydrogen Energy • 2023

Saikat Dutta, Rahul Patil, Tapan Dey

Nano Energy • 2022

Rebeca M. Torrente‐Rodríguez, Víctor Ruiz‐Valdepeñas Montiel, Susana Campuzano et al.

ACS Sensors • 2016

Microribonucleic acids (miRNAs) have been linked with various regulatory functions and diseases and constitute important targets in future medical diagnostics and prognostics. We report here a novel sensitive and rapid bioelectrochemical strategy for miRNA determination. This strategy involves the development of a sensing approach making use of magnetic beads (MBs) modified with a specific DNA-RNA antibody as capture bioreceptor and amperometric detection implying the H2O2/hydroquinone (HQ) system at disposable screen-printed carbon electrodes (SPCEs). The developed biosensor exhibits a dynamic range from 8.2 to 250 pM and a detection limit of 2.4 pM (60 amol) of a synthetic target without any amplification step in 2 h. The usefulness of the approach was evaluated by analyzing total RNA (RNAt) extracted from metastatic cancer cell lines and human tumor tissues, which demonstrated its potential to perform determination of mature miRNAs in these complex samples. Moreover, the feasibility of the developed methodology to detect simultaneously the expression of two different miRNAs at dual SPCEs (SPdCEs) in one single experiment was also explored. The feasibility to capture and release target miRNAs make the developed methodology also an attractive tool to isolate, purify, and determine target miRNAs with great applicability in the clinical field.

Graziela C. Sedenho, Guilherme Henrique Siqueira Ghiraldelli, Rodrigo M. Iost et al.

Sustainable Energy & Fuels • 2025

Bioelectrochemical systems (BESs) and engineered living materials (ELMs) are revolutionizing sustainable energy and carbon management by addressing thermodynamic and kinetic barriers in energy conversion and carbon capture.

Renata Toczyłowska‐Mamińska, K. Szymona, Patryk Maciej Król et al.

Energies • 2018

The abundance of cellulosic wastes make them attractive source of energy for producing electricity in microbial fuel cells (MFCs). However, electricity production from cellulose requires obligate anaerobes that can degrade cellulose and transfer electrons to the electrode (exoelectrogens), and thus most previous MFC studies have been conducted using two-chamber systems to avoid oxygen contamination of the anode. Single-chamber, air-cathode MFCs typically produce higher power densities than aqueous catholyte MFCs and avoid energy input for the cathodic reaction. To better understand the bacterial communities that evolve in single-chamber air-cathode MFCs fed cellulose, we examined the changes in the bacterial consortium in an MFC fed cellulose over time. The most predominant bacteria shown to be capable electron generation was Firmicutes, with the fermenters decomposing cellulose Bacteroidetes. The main genera developed after extended operation of the cellulose-fed MFC were cellulolytic strains, fermenters and electrogens that included: Parabacteroides, Proteiniphilum, Catonella and Clostridium. These results demonstrate that different communities evolve in air-cathode MFCs fed cellulose than the previous two-chamber reactors.

Ji Wang, Boyi Zhou, Ruijia Ge et al.

RSC Advances • 2018

The wide presence of antibiotics in the environment has raised concerns about their potential impact on ecological and human health. This study was conducted to evaluate the degradation of antibiotics (chlortetracycline (CTC) and oxytetracycline (OTC)) in microbial fuel cells (MFCs) and the change of toxicity. The degradation rates of 60 mg L -1 CTC and OTC in the MFCs were 74.2% and 78%, respectively, within 7 days. The degradation ability of the two antibiotics followed the order of OTC > CTC. Toxicity test results of the zebrafish illustrated the toxicity of OTC and CTC was largely eliminated by MFC treatment. Furthermore, possible degradation pathways of CTC and OTC were speculated using LC-MS analysis. High-throughput sequencing analysis indicated that Petrimonas , Azospirillum , Dokdonella , Burkholderia and Stenotrophomonas were the predominant genera in the MFC anode biofilm. Therefore, this work is of great significance for future studies on the treatment of antibiotics in wastewater by MFCs.

Marco Hartl, Diego F. Bedoya-Ríos, Marta Fernández-Gatell et al.

The Science of The Total Environment • 2018

Sikandar I. Mulla, Qian Sun, Anyi Hu et al.

PLoS ONE • 2016

This study is aimed to assess the biodegradation of sulfadiazine (SDZ) and characterization of heavy metal resistance in three pure bacterial cultures and also their chemotactic response towards 2-aminopyrimidine. The bacterial cultures were isolated from pig manure, activated sludge and sediment samples, by enrichment technique on SDZ (6 mg L-1). Based on the 16S rRNA gene sequence analysis, the microorganisms were identified within the genera of Paracoccus, Methylobacterium and Kribbella, which were further designated as SDZ-PM2-BSH30, SDZ-W2-SJ40 and SDZ-3S-SCL47. The three identified pure bacterial strains degraded up to 50.0, 55.2 and 60.0% of SDZ (5 mg L-1), respectively within 290 h. On the basis of quadrupole time-of-flight mass spectrometry and high performance liquid chromatography, 2-aminopyrimidine and 4-hydroxy-2-aminopyrimidine were identified as the main intermediates of SDZ biodegradation. These bacteria were also able to degrade the metabolite, 2-aminopyrimidine, of the SDZ. Furthermore, SDZ-PM2-BSH30, SDZ-W2-SJ40 and SDZ-3S-SCL47 also showed resistance to various heavy metals like copper, cadmium, chromium, cobalt, lead, nickel and zinc. Additionally, all three bacteria exhibited positive chemotaxis towards 2-aminopyrimidine based on the drop plate method and capillary assay. The results of this study advanced our understanding about the microbial degradation of SDZ, which would be useful towards the future SDZ removal in the environment.

Ewa Wojciechowska, Joanna Strycharz, Nicole Nawrot et al.

The Science of The Total Environment • 2024

Exponential increases in energy consumption and wastewater have often irreversible environmental impacts. As a result, bio-electrochemical devices like microbial fuel cells (MFCs), which convert chemical energy in organic matter to electricity using exoelectrogenic bacteria, have gained interest. However, operational factors affecting efficiency and energy output need further study. This research investigated bioenergy production and COD, TN, and TP removal in mesoscale floating treatment wetlands (FTW-MFC) using Phragmites australis, Iris pseudacorus, and a mix of both. The Iris FTW-MFC achieved a high voltage peak of 2100 mV. The maximum power densities of 484 mW/m 2 , 1196 mW/m 2 , and 441 mW/m 2 were observed for Phragmites, Iris, and mixed FTW-MFCs, respectively. Despite promising bioenergy yields, pollutant removal was unsatisfactory. A low area/height ratio (0.38 m 2 /0.8 m) and high loading rate (18.1 g/m 2 ·d COD) boosted bioenergy output but hindered treatment performance and stressed plants, causing root decay. No significant pollutant removal differences were found between FTW-MFC and FTW. Higher relative plant growth rates occurred in the FTW-MFC. Microbial analysis shown that representatives of Pseudomonas and Clostridium species were consistently found across all samples, involved in both organic compound transformation and electricity generation, contributed to successful microscale results. A supporting microscale MFC experiment showed wastewater composition's impact on bioenergy yield and pollutant removal. Pre-inoculated reactors improved organic matter transformation and electricity generation, while aeration increased voltage and treatment performance. The role of plants requires further verification in future experiments.

Jiahao Deng, Guang-yao Zhao, Fengyu Wang et al.

Bioprocess and Biosystems Engineering • 2022

Xiaojing Li, Xin Wang, Yueyong Zhang et al.

Scientific Reports • 2016

The extreme salinity and high internal resistance of saline-alkali soil contaminated by petroleum hydrocarbons were two key limitations for using the bioelectrochemical remediation. In order to solve two problems, we simply rinsed soil, added carbon fiber to polluted soil. The charge output was enhanced by 110% with increase of the maximum current densities from 81 to 304 mA·m(-2) while hydrocarbons degradation rate enhanced by 484%, especially the high molecular weight fractions (C28-C36 of n-alkanes and 4-6 rings of PAHs). These effects were possibly due to the selective enrichment of species belonged to δ-Proteobacteria (Proteobacteria), Flavobacteriia (Bacteroidetes) or Clostridia (Firmicutes), the activities of biological electron transfer and enzymes. As we know, oxygenase gene that directly decided the process of degradation, was surveyed for the first time in soil bioelectrochemical remediation system. The results confirmed that the bio-current stimulated the activities of naphthalene dioxygenase and xylene monooxygenase and thus the hydrocarbons degradation and the electricity generation. Given that electricity generation and the remediation performance are governed by multiple factors, understanding of microbial community and enzyme gene is crucial to promote the power yield and the bioelectrochemical remediation applicability.

So Ishizaki, Rimana Islam Papry, Hiroshi Miyake et al.

Frontiers in Microbiology • 2019

Integrated microbial fuel cell (MFC) and membrane bioreactor (MBR) systems are a promising cost-effective and energy-saving technology for wastewater treatment. Membrane fouling is still an important issue of such integrated systems in which aeration (oxygen) is replaced with anode electrodes (anodic respiration). Here, we investigated the effect of culture conditions on the membrane fouling potential of fouling-causing bacteria (FCB). In the present study, Klebsiella quasipneumoniae strain S05, which is an exoelectrogenic FCB isolated from a MBR treating municipal wastewater, was cultured with different external electron acceptors (oxygen, nitrate, and solid-state anode electrode). As results, the fouling potential of S05 was lowest when cultured with anode electrode and highest without any external electron acceptor ( p < 0.05, respectively). The composition of soluble microbial products (SMP) and extracellular polymeric substances (EPS) was also dependent on the type of electron acceptor. Protein and biopolymer contents in SMP were highly correlated with the fouling potential ( R 2 = 0.73 and 0.81, respectively). Both the fouling potential and yield of protein and biopolymer production were significantly mitigated by supplying electron acceptors sufficiently regardless of its types. Taken together, the aeration of MBR could be replaced with solid-state anode electrodes without enhancement of membrane fouling, and the anode electrodes must be placed sufficiently to prevent the dead spaces in the integrated reactor.

Jérémie-Luc Sanchez, David Pinto, Christel Laberty‐Robert

Electrochimica Acta • 2021

Sudipa Bhadra, Vijaya Raghavan, Surajbhan Sevda

Environmental Science and Pollution Research • 2025

Rongyao Dong, Xiya Lou, Zhijun Chen

Biosensors and Bioelectronics • 2024

Leilei Xiao, Fanghua Liu, Éric Lichtfouse et al.

Chemical Engineering Journal • 2020