Kun Dai, Jun‐Li Wen, Fang Zhang et al.

Bioresource Technology • 2017

Mengjie Fan, Wei Zhang, Jingyun Sun et al.

International Journal of Hydrogen Energy • 2017

Maedeh Mohammadifar, Seokheun Choi

Advanced Materials Technologies • 2017

Portable, on‐demand micropower generation is provided by developing paper‐based biobatteries that can deliver on‐chip energy to the next generation of point‐of‐care (POC) diagnostic platforms. This work creates a low‐cost, disposable, long shelf life and eco‐friendly micropower source that can be easily integrated in paper‐based POC devices and be readily activated by one drop of saliva, which is readily available in any challenging area. A high‐performance, paper‐based, bacteria‐powered battery is created by building microbial fuel cells with inactive, lyophilized (or freeze‐dried) exoelectrogenic cells, allowing for a long shelf life, which generates power within minutes of adding saliva. An oxygen‐tight interface and engineered conductive paper reservoir achieve significant performance boosts from maximized microbial electron transfer efficiency. Exoelectrogenic bacteria preinoculated in the paper battery is freeze‐dried for long‐term storage (in this work, the bacteria cells are stored for up to four months) and can be readily rehydrated for on‐demand power generation. Sixteen microbial fuel cells are incorporated on a single sheet of paper while all are connected in series with two electrical switches mounted on a paper circuit board, which produce more than enough electrical energy to power an on‐chip, light‐emitting diode.

Hamdan Z. Hamdan, Darine A. Salam, Ananda Rao Hari et al.

The Science of The Total Environment • 2016

Shao-Song Wu, Marcela Hernández, Yongcui Deng et al.

FEMS Microbiology Ecology • 2019

Previous studies showed that exoelectrogenic bacteria in paddy soil could suppress methanogens and methanogenesis after they were enriched by application of Fe3+ or running microbial fuel cells (MFCs). However, the relationship between exoelectrogenic bacteria and methanogens without the enrichment process is unknown. Our study was conducted in three paddy fields in China and over three seasons. We explored novel MFC-based sensors to in situ detect voltage signals that were generated from paddy soil within 10 min. The voltage and methane emission flux were determined as an indicator of the exoelectrogenic activity and methanogenic activity, respectively. The abundance of exoelectrogenic bacteria was assessed by quantifying five exoelectrogenic bacterial-associated genera including Geobacter, Shewanella, Anaeromyxobacter, Desulfovibrio and Clostridium, while the methanogens were studied by quantifying and sequencing the mcrA gene. The results showed that the abundance of exoelectrogenic bacteria and the voltage signals were positively correlated to the abundance of mcrA gene and methane emission flux, respectively. Moreover, non-metric dimensional scaling reveals that the abundance of Geobacter, Desulfovibrio and Clostridium significantly correlated with that of Methanomassiliicoccus, Methanoregula and Methanolinea. The present study suggests that the voltage signals might act as a novel indicator of methane emission flux in paddy fields.

Yan Yang, Yaqian Zhao, Cheng Tang et al.

Journal of Cleaner Production • 2021

Xu FangCheng, Zhi-Yi Mou, Jiya Geng et al.

Chemosphere • 2016

Tao Hua, Shengnan Li, Fengxiang Li et al.

Biochemical Engineering Journal • 2019

Zhiqiang Zhao, Yaobin Zhang, Weican Ma et al.

Biochemical Engineering Journal • 2016

Huihui Zhou, Bing-Feng Liu, Qisong Wang et al.

Biotechnology for Biofuels • 2017

The pulse electromagnetic field (PEMF) showed significant influence on state-of-the-art pulse magnetic bioelectrochemical systems (PEMF-MBES) in terms of current generation and microbial ecology. EET was instantaneously and reversibly enhanced in MBESs inoculated with either mixed-culture or Geobacter . PEMF notably decreased bacterial and archaeal diversities of the anode biofilms in MMFCs via changing species evenness rather than species richness, and facilitated specific enrichment of exoelectrogenic bacteria ( Geobacter ) on the anode surface. This study demonstrates a new magnetic approach for understanding and facilitating microbial electrochemical activities.

Gahyun Baek, Heejung Jung, Jaai Kim et al.

Bioresource Technology • 2017

Drishti Dinesh Bhagchandanii, Rishi Pramod Babu, Jayesh M. Sonawane et al.

Fermentation • 2020

Electro-fermentation (EF) is an upcoming technology that can control the metabolism of exoelectrogenic bacteria (i.e., bacteria that transfer electrons using an extracellular mechanism). The fermenter consists of electrodes that act as sink and source for the production and movement of electrons and protons, thus generating electricity and producing valuable products. The conventional process of fermentation has several drawbacks that restrict their application and economic viability. Additionally, metabolic reactions taking place in traditional fermenters are often redox imbalanced. Almost all metabolic pathways and microbial strains have been studied, and EF can electrochemically control this. The process of EF can be used to optimize metabolic processes taking place in the fermenter by controlling the redox and pH imbalances and by stimulating carbon chain elongation or breakdown to improve the overall biomass yield and support the production of a specific product. This review briefly discusses microbe-electrode interactions, electro-fermenter designs, mixed-culture EF, and pure culture EF in industrial applications, electro methanogenesis, and the various products that could be hence generated using this process.

Haobin Huang, Shaoan Cheng, Jiawei Yang et al.

Chemical Engineering Journal • 2017

Qian Liu, Zhiyong Jason Ren, Cong Huang et al.

Biotechnology for Biofuels • 2016

This study proves for the first time that biohythane could be produced directly in biocathode MECs using waste sludge. MEC and alkali-pretreatment accelerated enrichment of hydrogenotrophic methanogen and hydrolysis of waste sludge. The results indicate syntrophic interactions among fermentative bacteria, exoelectrogenic bacteria and methanogenic archaea in MECs are critical for highly efficient conversion of complex organics into biohythane, demonstrating that MECs can be more competitive than conventional anaerobic digestion for biohythane production using carbohydrate-deficient substrates. Biohythane production from waste sludge by MEC provides a promising new way for practical application of microbial electrochemical technology.

Jie Zhou, Qiaoling Lu, Ziyu Wang et al.

Chemical Engineering Journal • 2025

Zong-Chuang Yang, Yuanyuan Cheng, Feng Zhang et al.

Environmental Science & Technology Letters • 2016

Exoelectrogenic bacteria (EEB) play important roles in biogeochemical cycling, environmental remediation, wastewater treatment, and bioenergy recovery. Methods for effectively and rapidly probing the abundance of EEB in environments are highly desirable. In this work, a novel approach that couples WO3 nanoclusters and the most probable number (MPN) method for rapid detection and enumeration of EEB was developed. This WO3–MPN approach allowed rapid and reliable estimation of the population size of two typical EEB, Shewanella oneidensis MR-1 and Geobacter sulfurreducens DL-1. In addition, it was successfully applied to detect and count EEB in environmental samples from the sediments of a freshwater lake (9.9 × 104 to 4.1 × 106 cells/g of dry sediment) and engineered samples of a municipal wastewater treatment plant (1.0 × 103 to 7.5 × 105 cells/mL). This work may facilitate better identification and practical applications of EEB in natural and engineered environments.

Yong Hu, David Rehnlund, Edina Klein et al.

ACS Applied Materials & Interfaces • 2020

The use of living microorganisms integrated within electrochemical devices is an expanding field of research, with applications in microbial fuel cells, microbial biosensors or bioreactors. We describe the use of porous nanocomposite materials prepared by DNA polymerization of carbon nanotubes (CNTs) and silica nanoparticles (SiNPs) for the construction of a programmable biohybrid system containing the exoelectrogenic bacterium Shewanella oneidensis . We initially demonstrate the electrical conductivity of the CNT-containing DNA composite by employment of chronopotentiometry, electrochemical impedance spectroscopy, and cyclic voltammetry. Cultivation of Shewanella oneidensis in the conductive materials shows that the exoelectrogenic bacteria populate the matrix of the conductive composite, while nonexoelectrogenic Escherichia coli remain on its surface. Moreover, the ability to use extracellular electron transfer pathways is positively correlated with the number of cells within the conductive synthetic biofilm matrix. The Shewanella -containing composite remains stable for several days and shows electrochemical activity, indicating that the conductive backbone is capable of extracting the metabolic electrons produced by the bacteria under strictly anoxic conditions and conducting them to the anode. Programmability of this biohybrid material system is demonstrated by on-demand release and degradation induced by a short-term enzymatic stimulus. We believe that the application possibilities of such biohybrid materials could even go beyond microbial biosensors, bioreactors, and fuel cell systems.

Huihui Zhou, Xiaoxue Mei, Bing-Feng Liu et al.

Biotechnology for Biofuels • 2019

The constructed magnetic MFCs obtained better performance compared with the non-magnetic MFC, in terms of voltage production, power density, and coulombic efficiency. The relative abundance of Geobacter spp. (one kind of exoelectrogen) was much higher in the magnetic MFCs. The optimal static magnetic field intensity for enriching exoelectrogens is around 80 mT. It is likely that the decrease of internal resistance, enrichment in exoelectrogens, and the syntrophic interactions between exoelectrogens and methanogens result in the enhanced performance of magnetic MFCs. This study provides a magnetic method for the enrichment of exoelectrogens, which can be extensively applied in bioelectrochemical systems.

Jun Wang, Huan Deng, Shao-Song Wu et al.

CATENA • 2018

Jun-Cheng Han, Feng Zhang, Lei Cheng et al.

Environmental Science & Technology Letters • 2017

The extensive use of roxarsone in the poultry and livestock industry has led to increasing arsenic contamination of soil and aquatic environments. Microbial activity, especially exoelectrogenic bacterium (EEB)-mediated roxarsone bioreduction, plays important roles in such a bioconversion. However, the biomolecular-level mechanism behind this process and the reduction pathways remain largely unclear. Herein, the rapid anaerobic reduction of roxarsone by several EEB was explored, and the degradation pathways were clarified by using Shewanella putrefaciens CN32 as the model. The knockout of undA/mtrC led to a 70% loss of the roxarsone bioreduction ability within the initial 48 h. Both extracellular and intracellular reductions occurred simultaneously, resulting in the production of As(III) as the main inorganic arsenic species. Adding anthraquinone 2,6-disulfonate as a mediator considerably increased the roxarsone reduction rate by 119%. Given the wide distribution of EEB in environments, our findings facilitate a better understanding of the transformation behaviors of arsenic compounds in natural environments and highlight the necessity of re-evaluating the environmental risks of roxarsone.

Heng Dong, Xiaowan Liu, Ting Xu et al.

Bioresource Technology • 2017

Amanda Prado de Nicolás, R. Berenguer, Abraham Esteve‐Núñez

Chemical Engineering Journal • 2022

Fanying Kong, Hong‐Yu Ren, Spyros G. Pavlostathis et al.

Chemical Engineering Journal • 2018

Gunda Mohanakrishna, Riyadh I. Al‐Raoush, Ibrahim M. Abu-Reesh et al.

RSC Advances • 2019

A pristine soil environment supports a healthy soil biodiversity, which is often polluted with recalcitrant compounds. The bioelectrochemical remediation of the contaminated soils using bioelectrochemical systems (BESs) is gaining significant attention with respect to the restoration of the soil ecosystem. In this direction, a microbial fuel cell (MFC, an application of BES), was employed for the treatment of total petroleum hydrocarbons (TPHs) in a soil microenvironment at three ranges of pollution (loading conditions - 320, 590 and 840 mg TPH per L). TPHs degraded effectively in the soil-electrode vicinity in the range of 158 mg TPH R per L (320 mg TPH per L) and 356 mg TPH R per L (840 mg TPH per L). The study also demosntrated a maximum bioelectrogenesis of 286.7 mW m -2 (448 mV at 100 Ω) at the highest TPH loading concentration studied (840 mg TPH per L). The conditions prevailing in the soil MFC also facilitated the removal of sulfates (114 mg SO 4 2- per L; 62.64%) and the removal of total dissolved solids (910 mg TDS per L, 12.08%) at an 840 mg TPH per L loading condition. The pH of the outlet wastewater prevailing in the mild alkaline range of 7.6 and 8.4, along with improved sulfate and TPH removal in the respective conditions suggested suitable conditions for sulfate-reducing bacteria (SRB). This study also signified the sustainability of the process for the effective treatment of hydrocarbon contaminated soil that also generates green energy.

Sufiyan Siddiqui, Pranshul Bhatnagar, Sahej Dhingra et al.

Biomass Conversion and Biorefinery • 2021

Wilgince Apollon, Iryna Rusyn, T. E. Kuleshova et al.

Journal of Water Process Engineering • 2024

Akshay Jain, Zhen He

Frontiers of Environmental Science & Engineering • 2018

Andrea G. Capodaglio, Silvia Bolognesi, Daniele Cecconet

Water • 2021

Nature (ecosystem) based processes for wastewater treatment include constructed wetlands (CWs), waste stabilization ponds, vegetated drainage ditches, buffer zones, instream or bankside river techniques, and mixotrophic systems, where light and CO2 are utilized, in addition to organic carbon compounds, by algal cultures. Algae-based systems can simultaneously remove organic matter, N, and P and may offer substantial energetic advantages compared to traditional biological treatment systems, require small spatial footprint, and contribute to biofuels production and CO2 emissions mitigation. Bioelectrochemical systems (BES) such as microbial fuel cells (MFCs) present characteristics compatible with the use in isolated realities for water and wastewater treatment with contextual energy recovery and may be combined with other nature-based process technologies to achieve good treatment and energy efficiencies. Despite that their application in real-scale plants has not been assessed yet, the most probable outcome will be the in situ/on site treatment (or pretreatment) of wastes for small “in house” plants not connected to the sewerage network. This paper focuses on the current practices and perspectives of hybrid nature-based systems, such as constructed wetlands and microalgae integrated phytoremediation plants, and their possible integration with microbial electrochemical technologies to increase recovery possibilities from wastes and positively contribute to a green economy approach.

Johanna M. Haavisto, Paolo Dessì, Pritha Chatterjee et al.

Chemical Engineering Journal • 2019

Tingting Zhu, Yaobin Zhang, Yiwen Liu et al.

The Science of The Total Environment • 2020

N’Dah Joel Koffi, Satoshi Okabe

Chemosphere • 2021

Yongguang Li, Yonggang Li, Bing‐Jie Ni et al.

Trends in biotechnology • 2025

Casper Borsje, Tom Sleutels, Michel Saakes et al.

Journal of Chemical Technology & Biotechnology • 2019

Abstract BACKGROUND Scaling up bioelectrochemical systems for the treatment of wastewater faces challenges. Material costs, low conductivity of wastewater and clogging are issues that need a novel approach. The granular capacitive moving bed reactor can potentially solve these challenges. In this reactor, capacitive activated carbon granules are used as bioanode material. The charge storage capabilities of these capacitive granules allow for the physical separation of the charging and the discharging process and therefore a separation of the wastewater treatment and energy recovery process. RESULTS This study investigates the performance of the granular capacitive moving bed reactor. In this reactor, activated granules were transported from the bottom to the top of the reactor using a gas lift and settled on top of the granular bed, which moved downwards through the internal discharge cell. This moving granular bed was applied to increase the contact time with the discharge anode to increase the current density. The capacitive moving bed reactor (total volume 7.7 L) produced a maximum current of 23 A m −2 normalized to membrane area (257 A m −3 granules ). Without granules, the current was only 1.4 A m −2 membrane . The activity of the biofilm on the granules increased over time, from 436 up to 1259 A m −3 granules . A second experiment produced similar areal current density and increase in activity over time. CONCLUSION Whereas the produced current density is promising for further scaling up of bioanodes, the main challenges are to improve the discharge of the charged granules and growth of biofilm on the granules under shear stress. © 2019 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Carolina Cruz Viggi, Stefania Casale, Habib Chouchane et al.

Journal of Chemical Technology & Biotechnology • 2019

Abstract BACKGROUND Microbial electrochemical technologies (METs) represent a novel platform to harvest the energy trapped in municipal wastewater. At the anode of METs, electro‐active bacteria (EAB) anaerobically oxidize wastewater constituents using the electrode as the terminal electron acceptor and, by so doing, generate an electric current. To convert complex wastewater constituents into electricity, EAB must not only establish syntrophic relationships with other members of the microbial community, but also compete with methanogens for consumption of hydrogen and acetate. Here, we examined the addition of magnetite nanoparticles (NPs) (250 mg Fe L −1 ) as a novel strategy to manipulate such metabolic interactions and in turn maximize the efficiency of wastewater treatment and the yield of electric current generation. RESULTS Batch experiments carried out either in the presence of a mixture of volatile fatty acids or of a synthetic sewage demonstrated that magnetite addition accelerate the rate of electrogenic oxidation of specific compounds, particularly propionate (up to 120%), an intermediate which frequently accumulates during anaerobic treatment processes, while correspondingly enhancing electric current generation (up to 90%), and diminishing the rate of competing methane generation (up to 50%). Notably, the composition of the microbial community was not substantially affected by the presence of magnetite nanoparticles, possibly suggesting that these latter facilitated extracellular electron transfer mechanisms (among microbes and with the electrode), rather than enriching conditions for specific microorganisms. CONCLUSION The addition of magnetite NPs may represent a practical strategy to kick‐start a bioelectrochemical system designed for wastewater treatment and improve the effectiveness of electrogenic substrate oxidation processes. © 2019 Society of Chemical Industry

Junhui Guo, Guiqin Yang, Zheng Zhuang et al.

The Science of The Total Environment • 2021

Hui Wang, Huiping Zeng, Junhong Zhang et al.

Journal of Hazardous Materials • 2025

Nuan Yang, Guoqiang Zhan, Daping Li et al.

Chemical Engineering Journal • 2018

Domestic wastewater treatment process via the activated sludge is complicated, energy-negative and potentially harmful. In this study, an up-flow bioelectrochemical filter reactor (UBEF) was designed without positive aeration in-priority for treating real domestic wastewater under different hydraulic retention times (HRTs). The removal of chemical oxygen demand (COD), ammonia and total nitrogen was attained at a high efficiency of 89%, 99% and 99% respectively, when HRT was set at h1 of similar to 2.53 d. However, with the decreased of HRT from 2.53 to 0.28 d, the removal efficiency of nitrogen and COD decreased to 50% and 40% respectively, but the maximum power density increased from 3.01 to 98.90 mW/m(3) with a low Coulombic efficiency of 0.25-1.51%. The results demonstrated that the UBEF can remove COD and ammonia from real domestic wastewater in an aeration-free energy sustainable process, although its power output was low. Moreover, the functional bacteria were detected using 16S rRNA gene-based pyrosequencing analysis, which showed that the microbial communities were different under varied HRT conditions. The Thauera-dominated consortium was inoculated in order to enhance the removal of pollutants and the generation of electricity from domestic wastewater, it was kept at a relative abundance when continuously feeding the real wastewater. In particular, nitrogen removing bacteria (NRB) including Nitrosomonas, Ignavibacterium, Thiobacillus, Dokdonella, Comamonas, Sterolibacterium and Flavobacterium were enriched on the anode and the cathode of the UBEF, which contributed to nitrogen removal

Enric Blázquez, Juan Antonio Baeza, David Gabriel et al.

The Science of The Total Environment • 2018

Ke Zhang, Siqiao Yang, Wei Wang et al.

Journal of Cleaner Production • 2022

Nuerla Ailijiang, Jiali Chang, Peng Liang et al.

Chemical Engineering Journal • 2020