Jingyi Zhang, Heyang Yuan, Yelin Deng et al.

Journal of Cleaner Production • 2018

Tahereh Jafary, Abdullah Al-Mamun, Halima Alhimali et al.

Desalination • 2020

Xi Chen, Haotian Sun, Peng Liang et al.

Journal of Power Sources • 2016

Tahereh Jafary, Abdullah Al-Mamun, Halimah Alhimali et al.

Renewable and Sustainable Energy Reviews • 2020

Carlo Santoro, Fernando Benito Abad, Alexey Serov et al.

Applied Energy • 2017

In this work, the electrodes of a microbial desalination cell (MDC) are investigated as the positive and negative electrodes of an internal supercapacitor. The resulting system has been named a supercapacitive microbial desalination cell (SC-MDC). The electrodes are self-polarized by the red-ox reactions and therefore the anode acts as a negative electrode and the cathode as a positive electrode of the internal supercapacitor. In order to overcome cathodic losses, an additional capacitive electrode (AdE) was added and short-circuited with the SC-MDC cathode (SC-MDC-AdE). A total of 7600 discharge/self-recharge cycles (equivalent to 44 h of operation) of SC-MDC-AdE with a desalination chamber filled with an aqueous solution of 30 g L -1 NaCl are reported. The same reactor system was operated with real seawater collected from Pacific Ocean for 88 h (15,100 cycles). Maximum power generated was 1.63 ± 0.04 W m -2 for SC-MDC and 3.01 ± 0.01 W m -2 for SC-MDC-AdE. Solution conductivity in the desalination reactor decreased by ∼50% after 23 h and by more than 60% after 44 h. There was no observable change in the pH during cell operation. Power/current pulses were generated without an external power supply.

Marina Ramírez-Moreno, Pau Rodenas, Martí Aliaguilla et al.

Frontiers in Energy Research • 2019

Microbial Desalination Cell (MDC) represents an innovative technology which accomplishes simultaneous desalination and wastewater treatment without external energy input. MDC technology could be employed to provide freshwater with low-energy input, for example, in remote areas where organic wastes (i.e., urban or industrial) are available. In addition, MDC technology has been proposed as pre-treatment in conventional reverse osmosis plants, with the aim of saving energy and avoiding greenhouse gases related to conventional desalination processes. The use of oxygen reduction (i.e. O 2 + 2H 2 O + 4e - 4 OH -, E 0 = 0.815 V, pH = 7) was usually implemented as cathodic reaction in most of the MDCs reported in literature, whereas other strategies based on liquid catholytes have been also proposed, for example, ferroferricyanide redox couple (i.e. Fe(CN) 3- 6 + 1e - Fe(CN) 4- 6 , E 0 = 0.37 V). As the MDC designs in the literature and operation modes (i.e., batch, continuous, semi-continuous, etc.) are quite different, the available MDC studies are not directly comparable. For this reason, the main objective of this work was to have a proper comparison of two similar MDCs operating with two different catholyte strategies, and compare performance and desalination efficiencies. In this sense, this study compares the desalination performance of two laboratory-scale MDCs located in two different locations for brackish water and sea water using two different strategies. The first strategy consisted of an air cathode for efficient oxygen reduction, while the second strategy was based on a liquid catholyte with Fe 3+ /Fe 2+ solution (i.e., ferro-ferricyanide complex). Both strategies achieved desalination efficiency above 90% for brackish water. Nominal desalination rates (NDR) were in the range of 0.17-0.14 Lm -2 h -1 for brackish and seawater with air diffusion cathode MDC, respectively, and 1.5-0.7 Lm -2 h -1 when using ferro-ferricyanide redox MDC. Organic matter present in wastewater was effectively removed at 0.9 and 1.1 kg CODm -3 day -1 using the air diffusion cathode MDC for brackish and Ramrez-Moreno et al.

Surajbhan Sevda, Ibrahim M. Abu-Reesh, Heyang Yuan et al.

Energy Conversion and Management • 2016

Yuxiang Liang, Huajun Feng, Dongsheng Shen et al.

Electrochimica Acta • 2016

Yue Dong, Junfeng Liu, Mingrui Sui et al.

Journal of Hazardous Materials • 2016

A. Carmalin Sophia, V.M. Bhalambaal, Éder C. Lima et al.

Journal of environmental chemical engineering • 2016

Morvarid Khazraee Zamanpour, Hamid‐Reza Kariminia, M Vosoughi

Journal of environmental chemical engineering • 2016

Xiaochun Chen, Guiqin Yang, Xiaoyun Quan et al.

The Science of The Total Environment • 2024

Yarui Liu, Xuemei Zhu, Qian Zhao et al.

iScience • 2021

The biosynthesis of metal nanoparticles from precious metals has been of wide concern. Their antibacterial activity is a main bottleneck restricting the bacterial activity and reduction performance. Here, bio-electrochemical systems were used to harvest electroactive biofilms (EABs), where bacteria were naturally protected by extracellular polymeric substances to keep activity. The biofilm was further encapsulated with polydopamine (PDA) as additional shield. Silver nanoparticles (AgNPs) were biosynthesized on EABs, whose electroactivity could be fully recovered after Ag + reduction. The PDA increased bacterial viability by 90%-105%, confirmed as an effective protection against antibacterial activity of Ag + /AgNPs. The biosynthetic process changed the component and function of the microbial community, shifting from bacterial Fe reduction to archaeal methanogenesis. These results demonstrated that the electrochemical acclimation of EABs and encapsulation with PDA were effective protective measures during the biosynthesis of AgNPs. These approaches have a bright future in the green synthesis of nanomaterials, biotoxic wastewater treatment, and sustainable bio-catalysis.

Fengjun Yin, Shun Lu, Cheng Song et al.

Biosensors and Bioelectronics • 2022

Alexandrе Loukanov, Anatoliy Angelov, Yuki Takahashi et al.

Colloids and Surfaces A Physicochemical and Engineering Aspects • 2019

Qi Zhao, Han Lv, Zhicheng Cui et al.

ACS ES&T Water • 2022

Electroactive bacteria (EAB) could drive the Fenton reaction for purifying wastewater and recovering power simultaneously. However, the toxicity could damage EAB and reduce extracellular electron transfer (EET) efficiency, leading to declined current generation and more Fe sludge production. This study hypothesizes that electron shuttle riboflavin could enhance the synergy of EAB and the Fenton reaction, thereby improving refractory degradation and power generation under refractory stress. A microbial electro-Fenton (MEF) system employing 4-chlorophenol (4-CP) as the model toxic pollutant was set up, and 3 μM riboflavin was supplemented. The results showed that the total resistance reduced by 14%, and the current output and Fe(II) proportion improved by 25% and 38%, respectively. Due to the supplementation of riboflavin, the 4-CP degradation kinetic constant increased by 3.8 times, and the two-component system upregulation and extracellular polymer synthesis improvement contributed to the acceleration of biofilm formation. The EET processes via electron shuttle and nanowire pathways were improved with riboflavin addition, achieving the promotion of the electro-Fenton reaction and the production of less toxic intermediates. The downregulation of the microbial antioxidant enzyme system (with vs without riboflavin) also confirmed the elimination of toxic stress. This study provided a feasible strategy to improve the performance of MEF reactors.

Peng Zhang, Chenhui Yang, Li Zeng et al.

Electrochimica Acta • 2022

Yi Lu, Xinyuan He, Huahua Li et al.

Chemical Engineering Journal • 2025

Jie Cheng, Xin Su, Meng Liu et al.

Environmental Pollution • 2023

Ginevra Giangeri, Panagiotis Tsapekos, Dimitra Pitsikoglou et al.

Chemical Engineering Journal • 2024

Direct interspecies electron transfer (DIET) stands as a cornerstone in anoxic ecosystems, facilitating electron exchange between partners without intermediates. However, a considerable gap persists in comprehensively elucidating how various materials promote DIET and interact with electroactive microbial species. To uncover the influence of different conductive materials on DIET-based anaerobic digestion systems, three carbon sources were used to simplify the microbial community and focus on exploring electroactive methanogens. Cyclic voltammetry results showed unique peaks in acetate-fed cultures with polyaniline-coated materials, highlighting the presence of DIET-active microbial species. CO2-fed cultures with polyaniline-coated materials exhibited robust electrotrophic methanogenic activity in electrochemical cells. Additionally, cyclic voltammetry provided insights into electroactive biofilm stratification on conductive materials, allowing for a nuanced interpretation of microbiome responses. Comparative genomics showcased Desulfobulbaceae sp. DTU28 involvement in DIET through the identification of e-pilin. Furthermore, a putative e-pilin protein sequence with high aromatic amino acidic content was detected in Aminobacterium sp. DTU61. Co-occurrence analysis revealed potential syntrophic interactions between Methanosarcina sp. DTU142 and other species like Firmicutes sp. DTU111 and Bacteria sp. DTU118, indicating potential DIET-based cooperations. By analyzing electrochemical data and microbial genomic information, this study elucidated the impact of each material on DIET activity and characterized electroactive species, providing a deeper understanding of DIET-based microbial community interactions.

Farah Mustafa Alrammah, Lingjun Xu, Niketan Patel et al.

The Science of The Total Environment • 2024

Samantha R. McCuskey, Glenn Quek, Ricardo Javier Vázquez et al.

Advanced Science • 2024

gLiving materials can achieve unprecedented function by combining synthetic materials with the wide range of cellular functions. Of interest are situations where the critical properties of individual abiotic and biotic elements improve via their combination. For example, integrating electroactive bacteria into conjugated polyelectrolyte (CPE) hydrogels increases biocurrent production. One observes more efficient electrical charge transport within the CPE matrix in the presence of Shewanella oneidensis MR-1 and more current per cell is extracted, compared to traditional biofilms. Here, the origin of these synergistic effects are examined. Transcriptomics reveals that genes in S. oneidensis MR-1 related to bacteriophages and energy metabolism are upregulated in the composite material. Fluorescent staining and rheological measurements before and after enzymatic treatment identified the importance of extracellular biomaterials in increasing matrix cohesion. The synergy between CPE and S. oneidensis MR-1 thus arises from initially unanticipated changes in matrix composition and bacteria adaption within the synthetic environment.

Mohammed Arroussi, Jiajia Wu, Liyang Zhu et al.

Bioelectrochemistry • 2024

Zhaoxin Zhou, Xinmin Liu, Ranran Chen et al.

Bioprocess and Biosystems Engineering • 2023

Yashmeen Budania, Smriti Mishra, Aradhana Mishra et al.

Chemical Engineering Journal • 2023

Austin J. Graham, Stephen L. Gibbs, Camila A. Saez Cabezas et al.

ChemElectroChem • 2021

Abstract Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on a variety of inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed on electrodes. To address this, we developed a platform for quantifying EET flux from cell suspensions using aqueous dispersions of infrared plasmonic tin‐doped indium oxide nanocrystals. Tracking the change in optical extinction during electron transfer enabled quantification of current generated by planktonic Shewanella oneidensis cultures. Using this method, we differentiated between starved and actively respiring cells, cells of varying genotype, and cells engineered to differentially express a key EET gene using an inducible genetic circuit. Overall, our results validate the utility of colloidally stable plasmonic metal oxide nanocrystals as quantitative biosensors in aqueous environments and contribute to a fundamental understanding of planktonic S. oneidensis electrophysiology using simple in situ spectroscopy.

Hao Cai, Yifei Niu, Tianyuan Guan et al.

Journal of Environmental Management • 2024

Jian‐Chun Ma, Nan Shi, Yezhen Zhang et al.

Journal of Power Sources • 2019

Lea Ouaknin Hirsch, Irina Amar Dubrovin, Bharath Gandu et al.

Bioelectrochemistry • 2023

The bacterial anode is a key factor for microbial fuel cell (MFC) performance. This study examined the potential of kaolin (fine clay) to enhance bacteria and conductive particle attachment to the anode. The bio-electroactivity of MFCs based on a carbon-cloth anode modified by immobilization with kaolin, activated carbon, and Geobacter sulfurreducens (kaolin-AC), with only kaolin (kaolin), and a bare carbon-cloth (control) anodes were examined. When the MFCs were fed with wastewater, the MFCs based on the kaolin-AC, kaolin, and bare anodes produced a maximum voltage of 0.6 V, 0.4 V, and 0.25 V, respectively. The maximum power density obtained by the MFC based on the kaolin-AC anode was 1112 mW‧m -2 at a current density of 3.33 A‧m -2 , 12% and 56% higher than the kaolin and the bare anodes, respectively. The highest Coulombic efficiency was obtained by the kaolin-AC anode (16%). The relative microbial diversity showed that Geobacter displayed the highest relative distribution of 64% in the biofilm of the kaolin-AC anode. This result proved the advantage of preserving the bacterial anode exoelectrogens using kaolin. To our knowledge, this is the first study evaluating kaolin as a natural adhesive for immobilizing exoelectrogenic bacteria to anode material in MFCs.

Feng-Ai Yang, Yanan Hou, Ce Cao et al.

Journal of Hazardous Materials • 2024

Vaanie Godbole, Simranjeet Singh, Praveen C. Ramamurthy et al.

Journal of environmental chemical engineering • 2023

Chengmei Liao, Qian Zhao, Shu Wang et al.

The Science of The Total Environment • 2021

Stéphane Pinck, Mathieu Etienne, Manuel Dossot et al.

Bioelectrochemistry • 2017

Leonardo Iannucci, Marco Parvis, Pierangela Cristiani et al.

IEEE Transactions on Instrumentation and Measurement • 2019

Materials corrosion in the presence of bacteria [microbiologically influenced corrosion, (MIC)] is an important issue for components deployed in industrial applications and marine environments, where unexpected and fast degradation is often observed. For these reasons, finding new testing methodologies to assess the behavior of different materials in these conditions is a topic of clear interest. This paper describes a new approach based on the use of microbial fuel cells, which are exploited to have known and controlled conditions during the test. The sample is immersed in an environment where the presence and activity of electroactive bacteria are easily monitored measuring the currents flowing between the electrodes of the cell. Then, after connecting the sample of the material under study, reactions occurring on its surface can be monitored and its corrosion resistance can be assessed. This novel methodology is simple, easy to deploy, and can be proposed to assess microbial corrosion resistance of metals and alloys, monitoring, at the same time, the biofilm grown on the metals surface.

Shuomeng Zhang, Lei Wang, Liang Wu et al.

Nano Letters • 2021

For bacterial adhesion and biofilm formation, a thorough understanding of the mechanism and effective modulating is lacking due to the complex extracellular electron transfer (EET) at bacteria-surface interfaces. Here, we explore the adhesion behavior of a model electroactive bacteria under various metabolic conditions by an integrated electrochemical single-cell force microscopy system. A nonlinear model between bacterial adhesion force and electric field intensity is established, which provides a theoretical foundation for precise tuning of bacterial adhesion strength by the surface potential and the direction and flux of electron flow. In particular, based on quantitative analyses with equivalent charge distribution modeling and wormlike chain numerical simulations, it is demonstrated that the chain conformation and unfolding events of outer membrane appendages are dominantly impacted by the dynamic bacterial EET processes. This reveals how the anisotropy of bacterial conductive structure can translate into the desired adhesion behavior in different scenarios.

Ping Wu, Peng Ding, Qihao Cao et al.

Chemical Engineering Journal • 2023

Debajyoti Bose, Himanshi Dhawan, Vaibhaw Kandpal et al.

Enzyme and Microbial Technology • 2018

Jinning Wang, Mei Chen, Jiayao Zhang et al.

Environmental Science and Ecotechnology • 2023

Bioelectrochemical systems (BES) have emerged as a dual-function technology for treating wastewater and recovering energy. A vital element of BES is the rapid formation and maintenance of electroactive biofilms (EABs). Previous attempts to accelerate EAB formation and improve electroactivities focused on enhancing the bacterial adhesion process while neglecting the rate-limiting step of the bacterial transport process. Here, we introduce membrane filtration into BES, establishing a dynamic membrane filtration system that enhances overall performance. We observed that optimal membrane flux considerably reduced the startup time for EAB formation. Specifically, EABs established under a 25 L m -2 h -1 flux (EAB 25 LMH ) had a formation time of 43.8 ± 1.3 h, notably faster than the 51.4 ± 1.6 h in the static state (EAB 0 LMH ). Additionally, EAB 25 LMH exhibited a significant increase in maximum current density, approximately 2.2 times higher than EAB 0 LMH . Pearson correlation analysis indicated a positive relationship between current densities and biomass quantities and an inverse correlation with startup time. Microbial analysis revealed two critical findings: (i) variations in maximum current densities across different filtration conditions were associated with redox-active substances and biomass accumulation, and (ii) the incorporation of a filtration process in EAB formation enhanced the proportion of viable cells and encouraged a more diverse range of electroactive bacteria. Moreover, the novel electroactive membrane demonstrated sustained current production and effective solid-liquid separation during prolonged operation, indicating its potential as a viable alternative in membrane-based systems. This approach not only provides a new operational model for BES but also holds promise for expanding its application in future wastewater treatment solutions.

Yuanfeng Liu, Guangming Zhou, Yaxin Sun et al.

Applied Surface Science • 2022

Xiaoqiu Lin, Linshan Zheng, Min Zhang et al.

Chemical Engineering Journal • 2022