Fan Chen, Yin Ye, Beilei Fan et al.

Chemical Engineering Journal • 2022

Teng Cai, Yizhi Zhang, Na Wang et al.

Chemical Engineering Journal • 2022

Tiantian Guo, Jingzhen Wang, Xiaodi Yu et al.

Biochemical Engineering Journal • 2022

Lakshmi Deepika Bharatula, Enrico Marsili, James Kwan

Electrochimica Acta • 2019

Zulema Borjas, Abraham Esteve‐Núñez, Juan Manuel Ortiz

Journal of Power Sources • 2017

Peng Ding, Ping Wu, Jie Zhang et al.

The Science of The Total Environment • 2021

Yolina Hubenova, Eleonora Hubenova, Mario Mitov

Bioelectrochemistry • 2020

Laura Rago, Sarah Zecchin, Federica Villa et al.

Bioelectrochemistry • 2018

Lean Zhou, Xuejun Yan, Yuqing Yan et al.

Chemical Engineering Journal • 2019

Teng Cai, Xueqin Lü, Zhongyi Zhang et al.

Journal of Cleaner Production • 2022

Weitong Ren, Zi-Lin He, Yang Lv et al.

Water Research • 2024

Lu Lü, Fernanda Leite Lobo, Defeng Xing et al.

Applied Energy • 2019

Yuqing Yan, Tian Li, Lean Zhou et al.

Journal of Power Sources • 2019

Jia Song, Yanhui Lu, Ting Pan et al.

Advanced Functional Materials • 2024

Abstract Utilization of electro‐responsive biomaterials with antibacterial properties is advantageous for facilitating septic wound healing and tissue regeneration. However, the dose‐response effects of electrical stimuli from these materials against bacteria are not rigorously characterized, and achieving synergy of bactericidal and pro‐regenerative effects of biomaterials remains a major challenge. Here, a graded series of flexible BaTiO 3 /P(VDF‐TrFE) electroactive nanocomposite membranes (EMs) are developed with varying surface charge intensities, to serve as antibacterial dressing for septic wound healing. EMs display broad‐spectrum antibacterial effects against both Gram‐positive and Gram‐negative bacteria in a dose‐dependent manner, depending on the magnitude of their surface electrical potential. Mechanistically, the surface charge of EMs increase intracellular levels of reactive oxygen species within bacteria cells, which in turn caused oxidative damage to the bacterial membrane, thereby suppressing bacterial activity and biofilm formation. Moreover, in vivo studies demonstrated that EMs effectively inhibited S. aureus infection and accelerated wound healing in a mouse skin defect model, as well as ameliorated P. gingivalis‐ mediated periodontal inflammation in a mouse periodontitis model. Hence, this study optimizes the antibacterial properties of electroactive materials and characterizes the dose‐response effects of surface electrical charge against bacteria, thus validating the therapeutic applications of electroactive biomaterials in combating bacterial infection.

Fan Chen, Beilei Fan, Chunlin Wang et al.

Journal of Hazardous Materials • 2022

Leire Caizán‐Juanarena, Julia R. Krug, Frank J. Vergeldt et al.

Water Research • 2019

The use of microbial fuel cells (MFCs) for wastewater treatment fits in a circular economy context, as they can produce electricity by the removal of organic matter in the wastewater. Activated carbon (AC) granules are an attractive electrode material for bioanodes in MFCs, as they are cheap and provide electroactive bacteria with a large surface area for attachment. The characterization of biofilm growth on AC granules, however, is challenging due to their high roughness and three-dimensional structure. In this research, we show that 3D magnetic resonance imaging (MRI) can be used to visualize biofilm distribution and determine its volume on irregular-shaped single AC granules in a non-destructive way, while being combined with electrochemical and biomass analyses. Ten AC granules with electroactive biofilm (i.e. granular bioanodes) were collected at different growth stages (3 to 21 days after microbial inoculation) from a multi-anode MFC and T 1 -weighted 3D-MRI experiments were performed for three-dimensional biofilm visualization. With time, a more homogeneous biofilm distribution and an increased biofilm thickness could be observed in the 3D-MRI images. Biofilm volumes varied from 0.4 μL (day 4) to 2 μL (day 21) and were linearly correlated (R 2 = 0.9) to the total produced electric charge and total nitrogen content of the granular bioanodes, with values of 66.4 C μL -1 and 17 μg N μL -1 , respectively. In future, in situ MRI measurements could be used to monitor biofilm growth and distribution on AC granules.

Ergin Taşkan, Selman BULAK, Banu Taşkan et al.

Bioelectrochemistry • 2019

Min‐Soo Kim, Shuwei Li, Da Seul Kong et al.

Chemosphere • 2022

Zi Song, Xiaoli Su, Pu Li et al.

Bioresource Technology • 2021

Nannan Zhao, Yuhang Liu, Yifeng Zhang et al.

Water Research • 2022

Xianyue Jing, Yuting Yang, Zhihao Ai et al.

The Science of The Total Environment • 2019

Jolien De Paepe, Kim De Paepe, Francesc Gòdia et al.

Water Research • 2020

Huan Wang, Lu Lü, Deqiang Mao et al.

Chemosphere • 2019

Beenish Saba, Ann D. Christy, Zhongtang Yu et al.

Bioelectrochemistry • 2016

Qing Du, Quanhua Mu, Tao Cheng et al.

Environmental Science & Technology • 2018

Exoelectrogens acclimated from the environment are the key to energy recovery from waste in bioelectrochemical systems. However, it is still unknown how these bacteria are selectively enriched on the electrode. Here we confirmed for the first time that the electric field (EF) intensity selects exoelectrogens from wastewater using an integrated electrovisual system with a gradient EF. Under the operating conditions ( I = 3 × 10 -3 A), the EF intensity on the working electrode ranged from 6.00 V/cm at the center to 1.08 V/cm at the edge. A thick biofilm (88.9 μm) with spherical pink aggregates was observed at the center, while the color became gray at the edge (33.8 μm). The coverage of the biofilm also increased linearly with EF intensity from 0.42 at the edge (12 mm to the center) to 0.78 at the center. The biofilm at the center contained 76% Geobacter, which was 25% higher than that at the edge (60%). Geobacter anodireducens was the main species induced by the EF (50% at the center vs 24% at the edge). These results improve our fundamental knowledge of exoelectrogen acclimation and mixed electroactive biofilm formation, which has broader implications for energy recovery from waste and general understanding of microbial ecology.

Elisabet Perona-Vico, Laura Feliu-Paradeda, Sebastià Puig et al.

Scientific Reports • 2020

Hydrogen is a key intermediate element in microbial electrosynthesis as a mediator of the reduction of carbon dioxide (CO 2 ) into added value compounds. In the present work we aimed at studying the biological production of hydrogen in biocathodes operated at - 1.0 V vs. Ag/AgCl, using a highly comparable technology and CO 2 as carbon feedstock. Ten bacterial strains were chosen from genera Rhodobacter, Rhodopseudomonas, Rhodocyclus, Desulfovibrio and Sporomusa, all described as hydrogen producing candidates. Monospecific biofilms were formed on carbon cloth cathodes and hydrogen evolution was constantly monitored using a microsensor. Eight over ten bacteria strains showed electroactivity and H 2 production rates increased significantly (two to eightfold) compared to abiotic conditions for two of them (Desulfovibrio paquesii and Desulfovibrio desulfuricans). D. paquesii DSM 16681 exhibited the highest production rate (45.6 ± 18.8 µM min -1 ) compared to abiotic conditions (5.5 ± 0.6 µM min -1 ), although specific production rates (per 16S rRNA copy) were similar to those obtained for other strains. This study demonstrated that many microorganisms are suspected to participate in net hydrogen production but inherent differences among strains do occur, which are relevant for future developments of resilient biofilm coated cathodes as a stable hydrogen production platform in microbial electrosynthesis.

Li Zeng, Peng Zhang, Ye Qiu et al.

The Science of The Total Environment • 2020

Nan Xiao, Rong Wu, Jinhui Jeanne Huang‬‬‬‬ et al.

Chemical Engineering Science • 2020

Jingting Wang, Guoqiang Zhan, Yang Xu et al.

Biosensors and Bioelectronics • 2022

Dandan Liang, Zeng Li, Guohong Liu et al.

Chemical Engineering Journal • 2022

Basem S. Zakaria, Bipro Ranjan Dhar

The Science of The Total Environment • 2020

Mengqi Zheng, Chunyan Xu, Dan Zhong et al.

Chemical Engineering Journal • 2019

Sara Tejedor‐Sanz, Jose Rodrigo Quejigo, Antonio Berná et al.

ChemSusChem • 2016

We have explored a new concept in bacteria-electrode interaction based on the use of fluid-like electrodes and planktonic living cells. We show for the first time that living in a biofilm is not a strict requirement for Geobacter sulfurreducens to exchange electrons with an electrode. The growth of planktonic electroactive G. sulfurreducens could be supported by a fluid-like anode as soluble electron acceptors and with electron transfer rates similar to those reported for electroactive biofilms. This growth was maintained by uncoupling the charge (catabolism) and discharge (extracellular respiration) processes of the cells. Our results reveal a novel method to culture electroactive bacteria in which every single cell in the medium could be instantaneously wired to a fluid-like electrode. Direct extracellular electron transfer is occurring but with a new paradigm behind the bacteria-electrode interaction.

Salvador Gomez‐Carretero, Ben Libberton, Karl Svennersten et al.

npj Biofilms and Microbiomes • 2017

Abstract Biofouling is a major problem caused by bacteria colonizing abiotic surfaces, such as medical devices. Biofilms are formed as the bacterial metabolism adapts to an attached growth state. We studied whether bacterial metabolism, hence biofilm formation, can be modulated in electrochemically active surfaces using the conducting conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT). We fabricated composites of PEDOT doped with either heparin, dodecyl benzene sulfonate or chloride, and identified the fabrication parameters so that the electrochemical redox state is the main distinct factor influencing biofilm growth. PEDOT surfaces fitted into a custom-designed culturing device allowed for redox switching in Salmonella cultures, leading to oxidized or reduced electrodes. Similarly large biofilm growth was found on the oxidized anodes and on conventional polyester. In contrast, biofilm was significantly decreased (52–58%) on the reduced cathodes. Quantification of electrochromism in unswitched conducting polymer surfaces revealed a bacteria-driven electrochemical reduction of PEDOT. As a result, unswitched PEDOT acquired an analogous electrochemical state to the externally reduced cathode, explaining the similarly decreased biofilm growth on reduced cathodes and unswitched surfaces. Collectively, our findings reveal two opposing effects affecting biofilm formation. While the oxidized PEDOT anode constitutes a renewable electron sink that promotes biofilm growth, reduction of PEDOT by a power source or by bacteria largely suppresses biofilm formation. Modulating bacterial metabolism using the redox state of electroactive surfaces constitutes an unexplored method with applications spanning from antifouling coatings and microbial fuel cells to the study of the role of bacterial respiration during infection.

Carlos A. Ramírez-Vargas, Carlos Alberto Arias, Pedro N. Carvalho et al.

The Science of The Total Environment • 2018

Juntao Zhao, Feng Li, Shutian Kong et al.

Advanced Science • 2023

Shewanella oneidensis is able to carry out extracellular electron transfer (EET), although its EET efficiency is largely limited by low flavin concentrations, poor biofilm forming-ability, and weak biofilm conductivity. After identifying an important role for riboflavin (RF) in EET via in vitro experiments, the synthesis of RF is directed to 837.74 ± 11.42 µm in S. oneidensis. Molecular dynamics simulation reveals RF as a cofactor that binds strongly to the outer membrane cytochrome MtrC, which is correspondingly further overexpressed to enhance EET. Then the cell division inhibitor sulA, which dramatically enhanced the thickness and biomass of biofilm increased by 155% and 77%, respectively, is overexpressed. To reduce reaction overpotential due to biofilm thickness, a spider-web-like hybrid biofilm comprising RF, multiwalled carbon nanotubes (MWCNTs), and graphene oxide (GO) with adsorption-optimized elongated S. oneidensis, achieve a 77.83-fold increase in power (3736 mW m -2 ) relative to MR-1 and dramatically reduce the charge-transfer resistance and boosted biofilm electroactivity. This work provides an elegant paradigm to boost EET based on a synthetic biology strategy and materials science strategy, opens up further opportunities for other electrogenic bacteria.

David Pinto, Thibaud Coradin, Christel Laberty‐Robert

Bioelectrochemistry • 2017

Tian Li, Lean Zhou, Yawei Qian et al.

Applied Energy • 2017

Song Wang, Mingyi Xu, Biao Jin et al.

Water Research • 2022

Exoelectrogenic biofilm and the associated microbial electrochemical processes have recently been intensively studied for water treatment, but their response to and interaction with polyethylene (PE) microplastics which are widespread in various aquatic environments has never been reported. Here, we investigated how and to what extent PE microplastics would affect the electrochemistry and microbiology of exoelectrogenic biofilm in both microbial fuel cells (MFCs) and microbial electrolysis cells (MECs). When the PE microplastics concentration was increased from 0 to 75 mg/L in the MECs, an apparent decline in the maximum current density (from 1.99 to 0.74 A/m 2 ) and abundance of electroactive bacteria (EAB) in the exoelectrogenic biofilm was noticed. While in the MFCs, the current output was not significantly influenced and the abundance of EAB lightly increased at 25 mg/L microplastics. In addition, PE microplastics restrained the viability of the exoelectrogenic biofilms in both systems, leading to a higher system electrode resistance. Moreover, the microbial community richness and the microplastics-related operational taxonomic units decreased with PE microplastics. Furthermore, the electron transfer-related genes (e.g., pilA and mtrC) and cytochrome c concentration decreased after adding microplastics. This study provides the first glimpse into the influence of PE microplastics on the exoelectrogenic biofilm with the potential mechanisms revealed at the gene level, laying a methodological foundation for the future development of efficient water treatment technologies.

Shanshan Chen, Xianyue Jing, Jiahuan Tang et al.

Biosensors and Bioelectronics • 2017