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Agronomy • 2021

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Frontiers in Environmental Engineering • 2025

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Nanoscale • 2018

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[object Object], [object Object], [object Object] et al.

IEEE Communications Surveys & Tutorials • 2015

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Frontiers in Nanotechnology • 2024

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Environmental Research and Technology • 2024

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World • 2025

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IEEE Access • 2022

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Nanotechnology for Environmental Engineering • 2022

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UWSpace (University of Waterloo) • 2008

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Journal of International Humanitarian Action • 2024

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[object Object], [object Object], [object Object] et al.

Carbon Neutral Systems • 2025

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Leuser Journal of Environmental Studies • 2025

Antarctica, a seemingly barren and icy wilderness, is home to a diverse array of microbial life that plays a critical role in sustaining its ecosystems. These resilient microorganisms drive nutrient cycling and carbon sequestration, but their function in global processes remains unclear. This pristine environment faces mounting threats from human activities, climate change, and increasing tourism. Contaminants, non-native species, and microplastics are increasingly reaching even the most remote regions, disrupting delicate microbial communities existing for millions of years. Antarctic microorganisms are not only ecologically significant but also valuable for biotechnological advancements, making their conservation imperative. Climate change exacerbates these threats, altering microbial habitats and promoting shifts in community structure. Tourism growth, though beneficial for education and economic reasons, poses significant challenges through biological and chemical contamination. Despite efforts under the Antarctic Treaty System to protect the region, there is a critical need for enhanced measures specifically targeting microbial conservation. This article underscores the importance of conserving Antarctic microbial diversity. It highlights the intricate microbial ecosystems and the urgency of implementing strategies such as stringent biosecurity measures, sustainable tourism practices, and comprehensive monitoring programs. Additionally, fostering international collaboration and research initiatives is vital for understanding and designing strategies to mitigate the impacts of environmental changes on microbial life. By prioritizing microbial conservation in policy frameworks and strengthening global cooperation, we can safeguard these unique ecosystems and ensure their resilience for future generations.

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Biotechnology for Biofuels and Bioproducts • 2022

Global concerns about climate changes and their association with the use of fossil fuels have accelerated research on biological fuel production. Biological hydrogen production from hemicellulose-containing waste is considered one of the promising avenues. A major economical issue for such a process, however, is the low substrate conversion efficiency. Interestingly, the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus can produce hydrogen from carbohydrate-rich substrates at yields close to the theoretical maximum of the dark fermentation process (i.e., 4 mol H2/mol hexose). The organism is able to ferment an array of mono-, di- and polysaccharides, and is relatively tolerant to high partial hydrogen pressures, making it a promising candidate for exploitation in a biohydrogen process. The behaviour of this Gram-positive bacterium bears all hallmarks of being adapted to an environment sparse in free sugars, which is further reflected in its low volumetric hydrogen productivity and low osmotolerance. These two properties need to be improved by at least a factor of 10 and 5, respectively, for a cost-effective industrial process. In this review, the physiological characteristics of C. saccharolyticus are analyzed in view of the requirements for an efficient hydrogen cell factory. A special emphasis is put on the tight regulation of hydrogen production in C. saccharolyticus by both redox and energy metabolism. Suggestions for strategies to overcome the current challenges facing the potential use of the organism in hydrogen production are also discussed.

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Scientific Reports • 2024

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Frontiers in Microbiology • 2014

In the last decades, the understanding of inclusion body biology and consequently, of their properties and potential biotechnological applications have dramatically changed. Therefore, the development of new purification protocols aimed to preserve those properties is becoming a pushing demand.

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Chemical Society Reviews • 2017

Producing sustainable anode materials for lithium-ion batteries (LIBs) through catalytic graphitization of renewable biomass has gained significant attention. However, the technology is in its early stages due to the bio-graphite's comparatively low electrochemical performance in LIBs. This study aims to develop a process for producing LIB anode materials using a hybrid catalyst to enhance battery performance, along with readily available market biochar as the raw material. Results indicate that a trimetallic hybrid catalyst (Ni, Fe, and Mn in a 1:1:1 ratio) is superior to single or bimetallic catalysts in converting biochar to bio-graphite. The bio-graphite produced under this catalyst exhibits an 89.28% degree of graphitization and a 73.95% conversion rate. High-resolution transmission electron microscopy (HRTEM) reveals the dissolution-precipitation mechanism involved in catalytic graphitization. Electrochemical performance evaluation showed that the trimetallic hybrid catalyst yielded bio-graphite with better electrochemical performances than those obtained through single or bimetallic hybrid catalysts, including a good reversible capacity of about 293 mAh g -1 at a current density of 20 mA/g and a stable cycle performance with a capacity retention of over 98% after 100 cycles. This study proves the synergistic efficacy of different metals in catalytic graphitization, impacting both graphite crystalline structure and electrochemical performance.

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[object Object], [object Object], [object Object] et al.

Frontiers in Microbiology • 2017

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Frontiers in Microbiology • 2014

[object Object], [object Object], [object Object] et al.

Global Change Biology • 2016

The coral microbiome conforms a proxy to study effects of changing environmental conditions. However, scarce information exists regarding microbiome dynamics and host acclimation in response to environmental changes associated to global-scale disturbances. We assessed El Niño Southern Oscillation (ENSO)-derived thermal anomalies shifts in the bacterial microbiome of Pacifigorgia cairnsi (Gorgoniidae: Octocorallia) from the remote island of Malpelo in the Tropical Eastern Pacific. Malpelo is a hot spot of biodiversity and lacks direct coastal anthropogenic impacts. We evaluated the community composition and predicted functional profiles of the microbiome during 2015, 2017 and 2018, including different phases of ENSO cycle. The bacterial community diversity and composition between the warming and cooling phase were similar, but differed from the neutral phase. Relative abundances of different microbiome core members such as Endozoicomonas and Mycoplasma mainly drove these differences. An acclimated coral holobiont is suggested not just to warm but also to cold stress by embracing similar microbiome shifts and functional redundancy that allow maintaining coral's viability under thermal stress. Responses of the microbiome of unperturbed sea fans such as P. cairnsi in Malpelo could be acting as an extended phenotype facilitating the acclimation at the holobiont level.

[object Object], [object Object], [object Object] et al.

Microbial Cell Factories • 2011

B. subtilis is an important organism in the biotechnological application. The efficient expression system is desirable in production of recombinant gene products in B. subtilis. Recently, we developed a new inducible expression system in B. subtilis, which directed by B. subtilis maltose utilization operon promoter Pglv. The system demonstrated high-level expression for target proteins in B. subtilis when induced by maltose. However, the system was markedly repressed by glucose. This limited the application of the system as a high-expression tool in biotechnology field. The aim of this study was to further improve the Pglv promoter system and enhance its expression strength.

[object Object], [object Object], [object Object] et al.

Frontiers in Bioscience-Landmark • 2025

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Microbial Cell Factories • 2012

[object Object], [object Object], [object Object] et al.

Nanoscale Research Letters • 2015

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Frontiers in Microbiology • 2019

Chemical methods of producing dyes involve extreme temperatures and unsafe toxic compounds. Application of oxidizing enzymes obtained from fungal species, for example laccase, is an alternative to chemical synthesis of dyes. Laccase can be replaced by fungal biomass acting as a whole-cell biocatalyst with properties comparable to the isolated form of the enzyme. The application of the whole-cell system simplifies the transformation process and reduces the time required for its completion. In the present work, four fungal strains with a well-known ability to produce laccase were tested for oxidation of 17 phenolic and non-phenolic precursors into stable and non-toxic dyes.

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Frontiers in Microbiology • 2014

Recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. Today, two recombinant expression systems are particularly well represented in the literature reporting on recombinant expression of specific genes. According to searches in the PubMed citation database, during the last 15 years 80% of all recombinant genes reported on in the literature were expressed in either the enterobacterium Escherichia coli or the methylotropic yeast Pichia pastoris. Nevertheless, some eukaryotic proteins are misfolded or inadequately posttranslationally modified in these expression systems. This situation demands identification of other recombinant expression systems that enable the proper expression of the remaining eukaryotic genes. As of now, a single universal system allowing expression of all target genes is still a distant goal. In this light, thorough experimental screening for systems that can yield satisfying quantity and quality of target protein is required. In recent years, a number of new expression systems have been described and used for protein production. Two systems, namely Drosophila melanogaster S2 insect cells and human embryonic kidney 293 (HEK293) cells stably expressing the EBNA-1 gene, show exceptional promise. The time has come to identify a few well-performing systems that will allow us to express, purify, and characterize entire eukaryotic genomes.