Folusho Francis Ajayi, Kyoung-Yeol Kim, Kyu-Jung Chae et al.

Photochemical & Photobiological Sciences • 2010

Bio-hydrogen production in light-assisted microbial electrolysis cell (MEC) with a dye sensitized solar cell (DSSC) was optimized by connecting multiple MECs to a single dye (N719) sensitized solar cell (V(OC) approx. 0.7 V). Hydrogen production occurred simultaneously in all the connected MECs when the solar cell was irradiated with light. The amount of hydrogen produced in each MEC depends on the activity of the microbial catalyst on their anode. Substrate (acetate) to hydrogen conversion efficiencies ranging from 42% to 65% were obtained from the reactors during the experiment. A moderate light intensity of 430 W m(-2) was sufficient for hydrogen production in the coupled MEC-DSSC. A higher light intensity of 915 W m(-2), as well as an increase in substrate concentration, did not show any improvement in the current density due to limitation caused by the rate of microbial oxidation on the anode. A significant reduction in the surface area of the connected DSSC only showed a slight effect on current density in the coupled MEC-DSSC system when irradiated with light.

Yu Hong Jia, Ji Youn Choi, Jae Hun Ryu et al.

Korean Journal of Chemical Engineering • 2010

Aijie Wang, Wenzong Liu, Nanqi Ren et al.

International Journal of Hydrogen Energy • 2010

Folusho F. Ajayi, Kyoung-Yeol Kim, Kyu-Jung Chae et al.

International Journal of Hydrogen Energy • 2009

Anders Thygesen, Massimo Marzorati, Nico Boon et al.

Applied Microbiology and Biotechnology • 2010

Aijie Wang, Wenzong Liu, Ye Deng et al.

Journal of Biotechnology • 2010

M.-F. Manuel, V. Neburchilov, H. Wang et al.

Journal of Power Sources • 2010

Wenzong Liu, Aijie Wang, Nanqi Ren et al.

Journal of Biotechnology • 2008

Aijie Wang, Dan Sun, Nanqi Ren et al.

Journal of Biotechnology • 2008

Aijie Wang, Dan Sun, Lihong Liu et al.

Journal of Biotechnology • 2008

Geoffrey K. Rader, Bruce E. Logan

International Journal of Hydrogen Energy • 2010

Nathan Wrana, Richard Sparling, Nazim Cicek et al.

Journal of Cleaner Production • 2010

Leandro Lorenzelli, Benno Margesin, Sergio Martinoia et al.

Biosensors and Bioelectronics • 2003

In the last decade, fundamental advances in whole cell based sensors and microsystems have established the extracellular acidification rate monitoring of cell cultures as an important indicator of the global cellular metabolism. Innovative approaches adopting advanced integrated sensor array-based microsystems represent an emerging technique with numerous biomedical applications. This paper reports a cell-based microsystem, for multisite monitoring of the physiological state of cell populations. The functional components of the microsystem are an ion sensitive field effect transistor (ISFET) array-based sensor chip and a CMOS integrated circuit for signal conditioning and sensor signal multiplexing. In order to validate the microsystem capabilities for in-vitro toxicity screening applications, preliminary experimental measurements with Cheratinocytes, and CHO cells are presented. Variations in the acidification rate, imputable to the inhibitory effect of the drug on the metabolic cell activity have been monitored and cell viability during long term measurements has been also demonstrated.

Haiyan Wang, Jiuhui Qu

Journal of Environmental Science and Health, Part A • 2003

Kirsten J. J. Steinbusch, Hubertus V. M. Hamelers, Joris D. Schaap et al.

Environmental Science & Technology • 2009

Wei Guo, Haiyun Lu, Naifei Hu

Electrochimica Acta • 2006

Bobak R. Azamian, Jason J. Davis, Karl S. Coleman et al.

Journal of the American Chemical Society • 2002

Metalloproteins and enzymes can be immobilized on SWNTs of different surface chemistry. The combination of high surface area, robust immobilization and inherent nanotube electrochemical properties is of promising application in bioelectrochemistry.

Stefanie Rubenwolf, Sven Kerzenmacher, Roland Zengerle et al.

Applied Microbiology and Biotechnology • 2010

Hong-xu Li, Dian-zuo Wang, Guan-zhou Qiu et al.

Journal of Central South University of Technology • 2004

Larisa V. Sigolaeva, Alexander Makower, Arkadi V. Eremenko et al.

Analytical Biochemistry • 2001

Bioelectrochemical analysis of neuropathy target esterase (NTE) and its inhibitors is based on the combination of the NTE-catalyzed hydrolysis of phenyl valerate and phenol detection by a tyrosinase carbon-paste electrode. The use of the tyrosinase electrode improves 10-fold the sensitivity of NTE detection in comparison with a spectrophotometric method. The tyrosinase electrode was found to be suitable for measurements in whole human blood where spectrophotometric detection is considerably restricted. The specificity of NTE in blood for mipafox and di-2-propyl phosphorofluoridate was close to that for neuronal NTE. The NTE-like activity in blood was determined to be 0.19 +/- 0.02 nmol/min/mg of protein.

Marianna Villano, Federico Aulenta, Costanza Ciucci et al.

Bioresource Technology • 2010

This study describes the performance of a microbial biocathode, based on a hydrogenophilic methanogenic culture, capable of reducing carbon dioxide to methane, at high rates (up to 0.055 + or - 0.002 mmol d(-1) mgVSS(-1)) and electron capture efficiencies (over 80%). Methane was produced, at potentials more negative than -650 mV vs. SHE, both via abiotically produced hydrogen gas (i.e., via hydrogenophilic methanogenesis) and via direct extracellular electron transfer. The relative contribution of these two mechanisms was highly dependent on the set cathode potential. Both cyclic voltammetry tests and batch potentiostatic experiments indicated that the capacity for extracellular electron transfer was a constitutive trait of the hydrogenophilic methanogenic culture. In principle, both electrons and carbon dioxide required for methane production could be obtained from a bioanode carrying out the oxidation of waste organic substrates.

Florentina-Daniela Munteanu, Patricia Ferreira, Francisco J. Ruiz-Dueñas et al.

Journal of Electroanalytical Chemistry • 2008

Sergio Martinoia, Nicola Rosso, Massimo Grattarola et al.

Biosensors and Bioelectronics • 2001

Halyna Shkil, Leonard Stoica, Kostyantyn Dmytruk et al.

Bioelectrochemistry • 2009

In general, L-lactate respiration is difficult to detect in living yeast cells due to the small activity of L-lactate oxidizing enzymes within the mitochondria. Genetically modified cells of methylotrophic yeast Hansenula polymorpha overproducing L-lactate:cytochrome c-oxidoreductase (EC 1.1.2.3, also known as flavocytochrome b(2), FC b(2)) were physically immobilized by means of a dialysis membrane onto various types of electrode materials in order to investigate the possibility of electrochemically detecting L-lactate respiration. It could be shown that in the case of genetically modified Hansenula polymorpha cells in contrast to cells from the parental strain, enhanced L-lactate-dependent respiration could be detected. Due to overproduction of FC b(2) the O(2) reduction current is decreased upon addition of L-lactate to the electrolyte solution. The electron transfer pathway in the L-lactate-dependent respiration process involves a cascade over three redox proteins, FC b(2), cytochrome c and Complex-IV, starting with L-lactate oxidation and ending with oxygen reduction. By means of selective inhibition of Complex IV with CN(-), lactate respiration could be proven for causing the decrease in the O(2) reduction.

Ted Larsson, Annika Lindgren, Tautgirdas Ruzgas et al.

Journal of Electroanalytical Chemistry • 2000

Eduardo Nicolau, Ileana González-González, Michael Flynn et al.

Advances in Space Research • 2009

Eduardo Nicolau, Ileana González-González, José Fonseca et al.

ECS Meeting Abstracts • 2010

Abstract not Available.

Caitlyn S. Butler, Peter Clauwaert, Stefan J. Green et al.

Environmental Science & Technology • 2010

Tom H. J. A. Sleutels, Hubertus V. M. Hamelers, Cees J. N. Buisman

Environmental Science & Technology • 2010

Low pH buffer capacity of waste streams limits further development of bioelectrochemical systems (BES) because accumulation of protons potentially leads to acidification of the anodic biofilm. Here we introduce a system that makes it possible to recover alkalinity in an extra recovery compartment. The system consisted of this extra compartment which was located between anode and cathode compartment. The compartment was separated from the anode by a cation exchange membrane and from the cathode by an anion exchange membrane, which made clean hydrogen production possible. To compensate for the charge movement as a result of the flow of electrons, both cations and hydroxyl ions moved into the new recovery compartment. When a synthetic waste stream was fed through this recovery compartment, both pH and conductivity increased. When this stream is then fed to the anode of the BES, no additional buffer was required to produce the same current (3.5 A/m(2)) at an applied voltage of 1 V.

Alexander G. Volkov, Tanya C. Dunkley, Simone A. Morgan et al.

Bioelectrochemistry • 2004

G. Kh. Turishcheva, I. A. Kazarinov, O. V. Ignatov et al.

Microbiology • 2005

The metabolism of glucose and lactose in E. coli K-12 cells has been studied using a bioelectrochemical (BEC) approach. The magnitude and the duration of the response of a BEC anode were found to be functions of the composition of nutrient media and the concentration of bacterial cells. The amount of electricity that is generated enzymatically during the metabolism of a particular substrate depends on the activity of the relevant enzymes. This suggests that the BEC approach can be used for evaluating the activity of particular enzyme systems.

Camilo Enrique La Rotta, Diogo Simon Werberich, Eliane DElia

ECS Meeting Abstracts • 2006

Abstract not Available.

Hiroaki WATANABE, Hideki NISHI, Hiroshi HAMANA et al.

Journal of Environmental Sciences • 2008

Tsuneo Tanaka, Masao Kuroda

Journal of Environmental Engineering • 2000

Xueyan Zhao, Xin Lu, William T.Y. Tze et al.

Biosensors and Bioelectronics • 2010

Yang Mu, Korneel Rabaey, René A. Rozendal et al.

Environmental Science & Technology • 2009

H ZHOU

Sensors and Actuators B: Chemical • 2004

Ramesh K. Goel, Joseph R.V. Flora

Environmental Engineering Science • 2005

Frank N. Crespilho, Alexandre J.C. Lanfredi, Edson R. Leite et al.

Electrochemistry Communications • 2009

F. Aulenta, L. Tocca, P. Reale et al.

Journal of Biotechnology • 2010