Ramandeep Singh, Srishti Chaudhary, Sukrampal Yadav et al.

Electrochimica Acta • 2022

Laura Rago, Denny Popp, John T. Heiker et al.

Electrochimica Acta • 2020

Alexiane Godain, Timothy M. Vogel, Pascal Fongarland et al.

Biosensors and Bioelectronics • 2023

Nannan Zhao, Laura Treu, İrini Angelidaki et al.

Environmental Science & Technology • 2019

A thick and electroactive biofilm is the key to the successful development of microbial electrochemical systems and technologies (METs). In this study, intact anaerobic granular sludge (AGS), which is a spherical and dense microbial association, was successfully demonstrated as a novel and efficient biocatalyst in METs such as microbial fuel cells. Three different strategies were explored to shift the microbial composition of AGS from methanogenic to exoelectrogenic microbes, including varying the external resistance and organic loading and manipulating the anode potential. Among all the strategies, only with positive anode potential, AGS was successfully shifted from methanogenic to exoelectrogenic conditions, as indicated by the significantly high current response (10.32 A/m 2 ) and 100% removal of organic carbon from wastewater. Moreover, the AGS bioanode showed no significant decrease in current generation and organic removal at pH 5, indicating good tolerance of AGS to acidic conditions. Finally, 16S rRNA sequencing revealed the enrichment of exoelectrogens and inhibition of methanogens in the microbial community of AGS after anode potential control. This study provides a proof of concept for extracting electrical energy from organic wastes by exoelectrogenic AGS along with simultaneous wastewater treatment and meanwhile opens up a new paradigm to create an efficient and cost-effective exoelectrogenic biocatalyst for boosting the industrial application of METs.

Laura Rago, Sarah Zecchin, Stefania Marzorati et al.

Bioelectrochemistry • 2017

Jing Cai, Qaisar Mahmood, Aqiang Ding et al.

Energy & Fuels • 2020

Microbial fuel cells (MFCs), capable of concurrently treating sulfide and nitrate while generating electricity, were evaluated with various influent sulfide to nitrate molar ratios (S:N ratios). All MFCs displayed a good substrate removal capacity regardless of the S:N ratio (i.e., 5:0, 5:1, 5:2, and 5:3) and produced nitrogen gas, elemental sulfur, and sulfate as major products, while low S:N ratios mediated high sulfate conversion. By setting the S:N ratio to 5:2, the electricity generation was maximized, which was consistent with cyclic voltammetry (CV) curves. Through high-throughput sequencing, the taxonomic distribution of a microbial community in suspended sludge was analyzed; the diversity indices and a principal component analysis (PCA) suggest that the S:N ratios may affect the microbial community in MFCs. However, a Pearson correlation analysis infers that the S:N ratios do not have a significant impact on the richness and diversity of the microbial communities in MFCs involved in the simultaneous treatment of sulfide and nitrate (p > 0.05).

Jixiang Zou, Zhishuai Yuan, Qinghuan Chang et al.

Renewable Energy • 2024

Qiao Yang, Shengna Liang, Jia Liu et al.

Catalysts • 2017

The anode electrode is one of the most important components in all microbial electrochemical technologies (METs). Anode materials pretreatment and modification have been shown to be an effective method of improving anode performance. According to mass loss analysis during carbon fiber heating, five temperatures (300, 450, 500, 600, and 750 °C) were selected as the pre-heating temperatures of carbon fiber brush anodes. Microbial fuel cell (MFC) reactors built up with these pre-heated carbon brush anodes performed with different power densities and Coulombic efficiencies (CEs). Two kinds of measuring methods for power density were applied, and the numerical values of maximum power densities diverged greatly. Reactors with 450 °C anodes, using both methods, had the highest power densities, and the highest CEs were found using 500 °C anode reactors. The surface elements of heat-treated carbon fibers were analyzed using X-ray photoelectron spectra (XPS), and C, O, and N were the main constituents of the carbon fiber. There were four forms of N1s at the surface of the polyacrylonitrile (PAN)-based carbon fiber, and their concentrations were different at different temperature samples. The microbial community of the anode surface was analyzed, and microbial species on anodes from every sample were similar. The differences in anode performance may be caused by mass loss and by the surface elements. For carbon brush anodes used in MFCs or other BESs, 450–500 °C preheating was the most suitable temperature range in terms of the power densities and CEs.

Shiyu Chen, Xinyu Wang, Xueyao Shi et al.

Chemosphere • 2024

Nuan Yang, Hong Liu, Xiaojun Jin et al.

The Science of The Total Environment • 2020

Haitao Xu, Ye Chen, Qing Wen et al.

Journal of Cleaner Production • 2024

Shuai Liu, Yuxiang Lu, Hui Chen et al.

Journal of Water Process Engineering • 2022

Ru Wang, Sizhuo Wan, Bing-Yin Liu et al.

Journal of Water Process Engineering • 2022

Debajyoti Bose, Riya Bhattacharya, Alivia Mukherjee

Biochemical Engineering Journal • 2023

Kristina Joksimović, Aleksandra Žerađanin, Danijela Randjelović et al.

Journal of Power Sources • 2020

Bhuvanendran Revamma Sreelekshmy, Rubina Basheer, S.M.A. Shibli

Journal of environmental chemical engineering • 2022

Ming Li, Minghua Zhou, Xiaoyu Tian et al.

Energy • 2021

Xizi Long, Xian Cao, Shentan Liu et al.

Water Air & Soil Pollution • 2019

Manisha Verma, Vishal Singh, Vishal Mishra

Journal of environmental chemical engineering • 2023

Chi‐Wen Lin, Chih‐Hung Wu, Yi‐Ying Lin et al.

Journal of the Taiwan Institute of Chemical Engineers • 2018

Chao Li, Miaomiao Luo, Shihua Zhou et al.

International Journal of Hydrogen Energy • 2020

Jiaxin Li, Chongchao Yao, Bo Song et al.

The Science of The Total Environment • 2022

Xiangming Hu, Jindi Liu, Weimin Cheng et al.

Environmental Research • 2023

The application of microbially induced carbonate precipitation (MICP) technology is critical, but many challenges remain. In this paper, a microbial fuel cell (MFC) is used to treat molasses wastewater, and the effluent is used as the substrate to promote the growth of urease-producing bacteria. The results showed that the maximum voltage of MFC was 500 mV, and the maximum power density was 169.86 mW/m 2 . The mineralization rate reached 100% on the 15 th day, and the mineralized product was calcite CaCO 3 . According to the microbial community analysis, the unclassified_Comamondaceae, Arcobacter, and Aeromonas, which could improve the OH - , signal molecular transmission and small molecular nutrients to promote the urease activity of urease-producing bacteria. The above conclusions provide a new way to reuse molasses wastewater efficiently and to apply MICP technology in dust suppression.

Liuqingying Yang, Li Fei, Qing Wen et al.

International Journal of Hydrogen Energy • 2022

Parini Surti, Suresh Kumar Kailasa, Arvind Kumar Mungray

Chemosphere • 2022

Jierong Zheng, Sufang Wang, Cristiano Varrone et al.

Environmental Research • 2022

Kim Rafaelle E. Reyes, Po‐Wei Tsai, Lemmuel L. Tayo et al.

Process Biochemistry • 2020

Xiang Qi, Enling Tian, Yiwei Ren et al.

Polymer Degradation and Stability • 2018

Zhenxing Ren, JI Gui-xia, Hongbo Liu et al.

Journal of environmental chemical engineering • 2022

Wei Guo, Yingying Chen, Liang Cui et al.

Bioelectrochemistry • 2023

Cunkuan Zhang, Xiaolan Zeng, Xiaotang Xu et al.

Chemosphere • 2024

Fei Xie, Bowei Zhao, Ying Cui et al.

Frontiers of Environmental Science & Engineering • 2021

Jing Lian, Xiu-lei Tian, Zifu Li et al.

International Journal of Hydrogen Energy • 2017

Juping You, Han Chen, Liangliang Xu et al.

Electrochimica Acta • 2021

Aaron Leininger, Matthew D. Yates, Mark Ramirez et al.

Biotechnology and Bioengineering • 2020

A microbial fuel cell (MFC) system containing modular half-submerged biocathode was operated for 6 months in an 800 L flow-through system with domestic wastewater. For the first time, spatial and temporal differences in biofilm communities were examined on large three-dimensional electrodes in a wastewater MFC. Biocathode microbial community analysis showed a specialized biofilm community with electrogenic and electrotrophic taxa forming during operation, suggesting potentially opposing electrode reactions. The anodic community structure shifted during operation, but no spatial differences were observed along the length of the electrode. Power output from the system was most strongly influenced by pH. Higher power densities were associated with the use of solids-dewatering filtrate with increased organic matter, conductivity, and pH. The results show that the biocathode was the rate-limiting step and that future MFC design should consider the effect of size, shape, and orientation of biocathodes on their community assembly and electrotrophic ability.

Boobalan Thulasinathan, James Obeth Ebenezer, Abhispa Bora et al.

International Journal of Energy Research • 2020

Summary The microbial fuel cell (MFC) has gained interest among the scientific community due to the feasibility of transforming organic wastes directly into electrical energy through biocatalysis or enzymatic bioelectrochemical reactions. In the present study, an effective coculture system, namely, Serratia marcescens AATB1 and Klebsiella pneumoniae AATB2, was used in the MFC system. The isolated strains, AATB1 and AATB2 were identified as biofilm producing bacteria. The experiments were performed in a two‐chambered MFC setup with septic tank wastewater as the substrate in a 5 days batch mode. Pure culture of S. marcescens AATB1 and K. pneumoniae AATB2, and their cocultures were able to produce energy with the maximum current densities of 728.85 ± 36 mA/m 2 , 642.19 ± 32 mA/m 2 and 869.11 ± 43 mA/m 2 , respectively, and the maximum power densities of 341.65 ± 17 mW/m 2 , 257.51 ± 12 mW/m 2 and 398.69 ± 19 mW/m 2 , respectively. During the process, cyclic voltammetry analysis has revealed the electrochemical behavior of the anodic biofilm. Approximately, 70.42% ± 3.52% of chemical oxygen demand removal was achieved during the process. Biofilm formation by the adhesion of microbes on the electrode surfaces was visualized by confocal laser scanning microscope and scanning electron microscope. All the three MFC systems were produced by biofilms containing extracellular polymeric substance from S. marcescens AATB1 (55 ± 2.75 μg/cm 2 of protein, 61 ± 3.05 μg/cm 2 of carbohydrate) and K. pneumoniae AATB2 (46 ± 2.30 μg/cm 2 of protein, 53 ± 2.65 μg/cm 2 of carbohydrate) and coculture (60 ± 3.00 μg/cm 2 of protein, 69 ± 3.45 μg/cm 2 of carbohydrate). Furthermore, the anode biofilm metabolites were identified by Gas Chromatography–Mass Spectrometry (GC‐MS) and Nuclear Magnetic Resonance NMR spectroscopy.

Xiang Qi, Bo Yi, Yiwei Ren et al.

Polymer Degradation and Stability • 2018

Boobalan Thulasinathan, Tamilmani Jayabalan, Murugan Sethupathi et al.

Journal of Hazardous Materials • 2021

Jixiang Zou, Qinghuan Chang, Zhishuai Yuan et al.

Journal of Power Sources • 2022

Johanna M. Haavisto, Marika Kokko, Chyi–How Lay et al.

International Journal of Hydrogen Energy • 2017