Research Library
Discover insights from thousands of peer-reviewed papers on microbial electrochemical systems
Discover insights from thousands of peer-reviewed papers on microbial electrochemical systems
Julien Dherbécourt, Hélène Falentin, Stéphane Canaan et al.
Microbial Cell Factories • 2007
Lipolysis is an important process of cheese ripening that contributes to the formation of flavour. Propionibacterium freudenreichii is the main agent of lipolysis in Emmental cheese; however, the enzymes involved produced by this species have not yet been identified. Lipolysis is performed by esterases (carboxylic ester hydrolases, EC 3.1.1.-) which are able to hydrolyse acylglycerols bearing short, medium and long chain fatty acids. The genome sequence of P. freudenreichii type strain CIP103027T was recently obtained in our laboratory.The aim of this study was to identify as exhaustively as possible the potential esterases in P. freudenreichii that could be involved in the hydrolysis of acylglycerols in Emmental cheese. The proteins identified were produced in a soluble and active form by heterologous expression in Escherichia coli for further study of their activity and specificity of hydrolysed substrates.
Dave Ow, Denis Lim, Peter Morin Nissom et al.
Microbial Cell Factories • 2009
A. Khelifa, S. Aoudj, S. Moulay et al.
Desalination and Water Treatment • 2009
Lawrence P. Wackett
Microbial Biotechnology • 2009
Maurizio Bettiga, Oskar Bengtsson, Bärbel Hahn-Hägerdal et al.
Microbial Cell Factories • 2008
Ahmad A Zeidan, Peter Rådström, Ed WJ van Niel
Microbial Cell Factories • 2009
Yang M Ming, Zhang W Wei, Chen Y Lin et al.
Microbial Cell Factories • 2009
B. subtilis is an important organism in the biotechnological application. The efficient expression system is desirable in production of recombinant gene products in B. subtilis. Recently, we developed a new inducible expression system in B. subtilis, which directed by B. subtilis maltose utilization operon promoter Pglv. The system demonstrated high-level expression for target proteins in B. subtilis when induced by maltose. However, the system was markedly repressed by glucose. This limited the application of the system as a high-expression tool in biotechnology field. The aim of this study was to further improve the Pglv promoter system and enhance its expression strength.
Germán L Rosano, Eduardo A Ceccarelli
Microbial Cell Factories • 2008
Daniel M Widmaier, Christopher A Voigt
Microbial Cell Factories • 2009
Ralf Heermann, Tim Zeppenfeld, Kirsten Jung
Microbial Cell Factories • 2007
Introducing point mutations into bacterial chromosomes is important for further progress in studies relying on functional genomics, systems- and synthetic biology, and for metabolic engineering. For many investigations, chromosomal systems are required rather than artificial plasmid based systems.
Chowdhurry Hasan, Kazuyuki Shimizu
Microbial Cell Factories • 2007
Juan F Martín, Eduardo Gudiña, José L Barredo
Microbial Cell Factories • 2007
Angel T. Zvezdov, Alla El Shemeri, Dilyana T. Zvezdova
Desalination and Water Treatment • 2009
Debasish Sarkar, Apratim Bhattacharya, Chiranjib Bhattacharjee
Desalination • 2010
J.M. Poyatosa, M. Molina-Muñoz, L. Fernandez et al.
Desalination and Water Treatment • 2009
Ilias Zafiriadis, Anastasios G. Kapagiannidis, Alexander Aivasidis
Desalination and Water Treatment • 2010
E.N. Peleka, P. Mavros, A.I. Zouboulis et al.
Desalination • 2009
Christoph Wannek, Andreas Glüsen, Detlef Stolten
Desalination • 2009
Asma Ben Rajeb, Hamadi Kallali, Nadhira Ben Aissa et al.
Desalination • 2009
Yanlin Hou, Yanan Gao, Ying Cai et al.
Desalination and Water Treatment • 2009
Noeon Park, Jinwook Lee, Kyongmi Chon et al.
Desalination and Water Treatment • 2008
Amira Ben Yahmed, Naila Saidi, Ismail Trabelsi et al.
Desalination • 2009
A. Galvão, J. Matos, M. Silva et al.
Desalination and Water Treatment • 2009
M.A. Darwish
Desalination and Water Treatment • 2010
Tuba Artan Onat, H. Tuğba Gümüşdere, Afife Güvenç et al.
Desalination • 2010
M.T. Montgomery, T.J. Boyd, C.L. Osburn et al.
Desalination • 2009
Y.J. Liu, X.C. Wang, H.L. Yuan
Desalination • 2009
C. Gorey, I.C. Escobar, C.L. Gruden et al.
Desalination • 2009
Ching-Jung Chuang, Chen-Yi Wu, Chia-Chun Wu
Desalination • 2008
David Eugene Kimbrough, Yao Kouame, Parvaneh Moheban et al.
International Journal of Environment and Pollution • 2005
A. Pohjoranta, A. Mendelson, R. Tenno
Electrochimica Acta • 2009
Hakan Dortay, Bernd Mueller-Roeber
Microbial Cell Factories • 2009
Ekaterina Osmekhina, Antje Neubauer, Katharina Klinzing et al.
Microbial Cell Factories • 2009
Chung-Hsien Cheng, Wen-Chien Lee
Microbial Cell Factories • 2009
Anne M Ruffing, Rachel R Chen
Microbial Cell Factories • 2009
Oligosaccharides containing a terminal Gal-alpha1,3-Gal moiety are collectively known as alpha-Gal epitopes. alpha-Gal epitopes are integral components of several medical treatments under development, including flu and HIV vaccines as well as cancer treatments. The difficulty associated with synthesizing the alpha-Gal epitope hinders the development and application of these treatments due to the limited availability and high cost of the alpha-Gal epitope. This work illustrates the development of a whole-cell biocatalyst for synthesizing the alpha-Gal epitope, Gal-alpha1,3-Lac.
Laura Salusjärvi, Matti Kankainen, Rabah Soliymani et al.
Microbial Cell Factories • 2007
Vicki Geiser, Suzanne Rose, Clinton Jones
Microbial Pathogenesis • 2008
Bovine herpesvirus 1 (BHV-1), a member of the alpha-herpesvirinae sub-family, causes significant losses to the cattle industry. BHV-1 establishes latency in trigeminal ganglionic sensory neurons, but periodically reactivates from latency. Previous studies suggested that infection with BHV-1-induced novel morphological changes in rabbit skin (RS) cells versus bovine kidney cells (MDBK). Consequently, we hypothesized that viral infection led to a novel form of cell death in RS cells compared to MDBK cells. To test this hypothesis, we examined the levels of apoptosis in these cell types following infection with BHV-1. Infection of RS, but not MDBK, cells leads to high levels of apoptosis compared to mock-infected cells. Previous studies indicated that a BHV-1 recombinant virus that does not express the bICP0 protein grows poorly in permissive cells and induces a persistent-like infection. This suggested that bICP0 played an important role in regulating cell death following infection. To test this hypothesis, we compared the levels of apoptosis in cells infected with the bICP0 null mutant versus viral strains that expressed bICP0. The bICP0 null mutant induces low levels of apoptosis in RS or MDBK cells. When MDBK cells are treated with UV light prior to infection, bICP0 expressing viral strains, but not the bICP0 null mutant, inhibited UV-induced apoptosis. Infection of MDBK cells with the bICP0 null mutant, leads to an accumulation of autophagosomes that are not detected following infection with bICP0 expressing viruses. These studies suggest that the bICP0 null mutant induces autophagy in MDBK cells, and bICP0 protein expression mediates cell-type specific cytotoxicity.
Bohumila Suchanova, Roman Tuma
Microbial Cell Factories • 2007
Špela Peternel, Joze Grdadolnik, Vladka Gaberc-Porekar et al.
Microbial Cell Factories • 2007
For a long time IBs were considered to be inactive deposits of accumulated target proteins. In our previous studies, we discovered IBs containing a high percentage of correctly folded protein that can be extracted under non-denaturing conditions in biologically active form without applying any renaturation steps. In order to widen the concept of correctly folded protein inside IBs, G-CSF (granulocyte colony stimulating factor) and three additional proteins were chosen for this study: GFP (Green fluorescent protein), His7dN6TNF-alpha (Truncated form of Tumor necrosis factor alpha with an N-terminal histidine tag) and dN19 LT-alpha (Truncated form of Lymphotoxin alpha).
Ario de Marco
Microbial Cell Factories • 2007
Structural characterization of proteins used in biological experiments is largely neglected. In most publications, the information available is totally insufficient to judge the functionality of the proteins used and, therefore, the significance of identified protein-protein interactions (was the interaction specific or due to unspecific binding of misfolded protein regions?) or reliability of kinetic and thermodynamic data (how much protein was in its native form?). As a consequence, the results of single experiments might not only become questionable, but the whole reliability of systems biology, built on these fundaments, would be weakened.The introduction of Minimal Information concerning purified proteins to add as metadata to the main body of a manuscript would render straightforward the assessment of their functional and structural qualities and, consequently, of results obtained using these proteins. Furthermore, accepted standards for protein annotation would simplify data comparison and exchange. This article has been envisaged as a proposal for aggregating scientists who share the opinion that the scientific community needs a platform for Minimum Information for Protein Functionality Evaluation (MIPFE).