Zhenwei Wang, Masashi Mori, Takuto Araki

International Journal of Hydrogen Energy • 2010

JaeHwa KOH, DuckJoo YOON, Chang H. OH

Journal of Nuclear Science and Technology • 2010

Maria Papagianni, Sofia Anastasiadou

Microbial Cell Factories • 2008

Andrew S Wieczorek, Vincent JJ Martin

Microbial Cell Factories • 2009

The assembly and spatial organization of enzymes in naturally occurring multi-protein complexes is of paramount importance for the efficient degradation of complex polymers and biosynthesis of valuable products. The degradation of cellulose into fermentable sugars by Clostridium thermocellum is achieved by means of a multi-protein "cellulosome" complex. Assembled via dockerin-cohesin interactions, the cellulosome is associated with the cell surface during cellulose hydrolysis, forming ternary cellulose-enzyme-microbe complexes for enhanced activity and synergy. The assembly of recombinant cell surface displayed cellulosome-inspired complexes in surrogate microbes is highly desirable. The model organism Lactococcus lactis is of particular interest as it has been metabolically engineered to produce a variety of commodity chemicals including lactic acid and bioactive compounds, and can efficiently secrete an array of recombinant proteins and enzymes of varying sizes.

Svenja T. Lohner, Andreas Tiehm

Environmental Science & Technology • 2009

Karin Willquist, Ahmad A Zeidan, Ed WJ van Niel

Microbial Cell Factories • 2009

Global concerns about climate changes and their association with the use of fossil fuels have accelerated research on biological fuel production. Biological hydrogen production from hemicellulose-containing waste is considered one of the promising avenues. A major economical issue for such a process, however, is the low substrate conversion efficiency. Interestingly, the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus can produce hydrogen from carbohydrate-rich substrates at yields close to the theoretical maximum of the dark fermentation process (i.e., 4 mol H2/mol hexose). The organism is able to ferment an array of mono-, di- and polysaccharides, and is relatively tolerant to high partial hydrogen pressures, making it a promising candidate for exploitation in a biohydrogen process. The behaviour of this Gram-positive bacterium bears all hallmarks of being adapted to an environment sparse in free sugars, which is further reflected in its low volumetric hydrogen productivity and low osmotolerance. These two properties need to be improved by at least a factor of 10 and 5, respectively, for a cost-effective industrial process. In this review, the physiological characteristics of C. saccharolyticus are analyzed in view of the requirements for an efficient hydrogen cell factory. A special emphasis is put on the tight regulation of hydrogen production in C. saccharolyticus by both redox and energy metabolism. Suggestions for strategies to overcome the current challenges facing the potential use of the organism in hydrogen production are also discussed.

Juan-Miguel Puertas, Jean-Michel Betton

Microbial Cell Factories • 2008

Despite advances in expression technologies, the efficient production of heterologous secreted proteins in Escherichia coli remains a challenge. One frequent limitation relies on their inability to be exported to the E. coli periplasm. However, recent studies have suggested that translational kinetics and signal sequences act in concert to modulate the export process.

Jacques M Mathieu, John Schloendorn, Bruce E Rittmann et al.

Microbial Cell Factories • 2008

Ario de Marco

Microbial Cell Factories • 2008

Soniya Dhanjal, Swaranjit Cameotra

Microbial Cell Factories • 2009

Tao Zhang, WenJun Han

Microbial Cell Factories • 2009

A metagenomic library was prepared using pCC2FOS vector containing about 3.0 Gbp of community DNA from the microbial assemblage of activated sludge. Screening of a part of the un-amplified library resulted in the finding of 1 unique lipolytic clone capable of hydrolyzing tributyrin, in which an esterase gene was identified. This esterase/lipase gene consists of 834 bp and encodes a polypeptide (designated EstAS) of 277 amino acid residuals with a molecular mass of 31 kDa. Sequence analysis indicated that it showed 33% and 31% amino acid identity to esterase/lipase from Gemmata obscuriglobus UQM 2246 (ZP_02733109) and Yarrowia lipolytica CLIB122 (XP_504639), respectively; and several conserved regions were identified, including the putative active site, HSMGG, a catalytic triad (Ser92, His125 and Asp216) and a LHYFRG conserved motif. The EstAS was overexpressed, purified and shown to hydrolyse p-nitrophenyl (NP) esters of fatty acids with short chain lengths (< or = C8). This EstAS had optimal temperature and pH at 35 degrees C and 9.0, respectively, by hydrolysis of p-NP hexanoate. It also exhibited the same level of stability over wide temperature and pH ranges and in the presence of metal ions or detergents. The high level of stability of esterase EstAS with its unique substrate specificities make itself highly useful for biotechnological applications.

Dominic Sauvageau, David G Cooper

Microbial Cell Factories • 2009

Thore Rohwerder, Roland H Müller

Microbial Cell Factories • 2009

Unknown Author

Membrane Technology • 2009

Esther Vázquez, Antonio Villaverde

Microbial Cell Factories • 2009

Chikashi Suzuki, Toshio Nakagiri, Kazumi Aoto

International Journal of Hydrogen Energy • 2007

Maurilio De Felice, Diethard Mattanovich, Maria Papagianni et al.

Microbial Cell Factories • 2008

Antonio Villaverde, Diethard Mattanovich

Microbial Cell Factories • 2006

Hans Sørensen

Microbial Cell Factories • 2009

Beate Pscheidt, Anton Glieder

Microbial Cell Factories • 2007

Jolanta Polak, Anna Jarosz-Wilkołazka

Microbial Cell Factories • 2009

Chemical methods of producing dyes involve extreme temperatures and unsafe toxic compounds. Application of oxidizing enzymes obtained from fungal species, for example laccase, is an alternative to chemical synthesis of dyes. Laccase can be replaced by fungal biomass acting as a whole-cell biocatalyst with properties comparable to the isolated form of the enzyme. The application of the whole-cell system simplifies the transformation process and reduces the time required for its completion. In the present work, four fungal strains with a well-known ability to produce laccase were tested for oxidation of 17 phenolic and non-phenolic precursors into stable and non-toxic dyes.

Zhijun Wang, Li Jin, Alicja Węgrzyn

Microbial Cell Factories • 2006

Leptospirosis is a serious infection disease caused by pathogenic strains of the Leptospira spirochetes, which affects not only humans but also animals. It has long been expected to find an effective vaccine to prevent leptospirosis through immunization of high risk humans or animals. Although some leptospirosis vaccines have been obtained, the vaccination is relatively unsuccessful in clinical application despite decades of research and millions of dollars spent. In this review, the recent advancements of recombinant outer membrane protein (OMP) vaccines, lipopolysaccharide (LPS) vaccines, inactivated vaccines, attenuated vaccines and DNA vaccines against leptospirosis are reviewed. A comparison of these vaccines may lead to development of new potential methods to combat leptospirosis and facilitate the leptospirosis vaccine research. Moreover, a vaccine ontology database was built for the scientists working on the leptospirosis vaccines as a starting tool.

Elena García-Fruitós

Microbial Cell Factories • 2009

Meng Ni

Chemical Engineering Journal • 2010

Unknown Author

Fuel and Energy Abstracts • 2000

Pau Ferrer

Microbial Cell Factories • 2006

Unknown Author

Fuel Cells Bulletin • 2004

Unknown Author

Fuel Cells Bulletin • 2001

Unknown Author

Fuel and Energy Abstracts • 2003

Unknown Author

Fuel and Energy Abstracts • 2002

Antonio Villaverde

Microbial Cell Factories • 2009

Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondary metabolites are now expected to become fully adapted for the improved biological production of nanostructured materials with tailored properties. The so far underestimated potential of microbial cell factories in nanotechnology and nanomedicine is expected to emerge, in the next years, in the context of novel needs envisaged in the nanoscience universe. This should prompt a careful revisiting of the microbial cell factories as the most versatile biological platforms to supply functional materials for nanotechnological applications.

P FEDORAK, D COY

Fuel • 2005

Uwe Schröder

ChemSusChem • 2008

Chenxin Cai, Jing Chen

Analytical Biochemistry • 2004

A stable suspension of carbon nanotube (CNT) can be obtained by dispersing the CNT in the solution of the surfactant cetyltrimethylammonium bromide. CNT has promotion effects on the direct electron transfer of hemoglobin (Hb), which was immobilized onto the surface of CNT. The direct electron transfer rate of Hb was greatly enhanced after it was immobilized onto the surface of CNT. Cyclic voltammetric results showed a pair of well-defined redox peaks, which corresponded to the direct electron transfer of Hb, with the formal potential (E(0('))) at about -0.343V (vs. saturated calomel electrode) in the phosphate buffer solution (pH 6.8). The electrochemical parameters such as apparent heterogeneous electron transfer rate constant (k(s)) and the value of formal potential (E(0('))) were estimated. The dependence of E(0(')) on solution pH indicated that the direct electron transfer reaction of Hb is a one-electron transfer coupled with a one-proton transfer reaction process. The experimental results also demonstrated that the immobilized Hb retained its bioelectrocatalytic activity to the reduction of H(2)O(2). The electrocatalytic current was proportional to the concentration of H(2)O(2) at least up to 20mM.

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Genes and environment : the official journal of the Japanese Environmental Mutagen Society • 2026

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Microbial cell factories • 2026

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Applied biochemistry and biotechnology • 2026

Journal of hazardous materials • 2025

A eukaryotic-bacterial symbiotic membraneless bioelectrochemical system (EBES) reactor with eukaryotic-bacteria symbiotic cathode was developed to treat swine wastewater containing enrofloxacin (ENR), which had high performance at ENR tolerance and operational stability. With ENR concentrations shifting from 2 to 50 mg/L, the removal efficiencies of ENR, chemical oxygen demand (COD) and NH 4 + -N always were higher than 95 %, and the maximum power output (≥343 mW/m 3 ) could be achieved. At 20 mg/L ENR, the removal efficiencies of ENR, COD and NH 4 + -N respectively reached to 99.4 ± 0.1 %, 98.5 % ± 0.1 %, and 96.3 % ± 0.5 %, corresponding to the open circuit voltage and maximum power density (P max ) of EBES were 851 mV and 455 mW/m 3 . The community analyses showed that bacteria (Comamonas, Rhodobacter, Rhodococcus, and Vermiphilaceae et al.), algae (Chlorella) and fungi (Rozellomycota, Trebouxiophyceae, Exophiala, and Aspergillus et al.) at genus level were the dominate populations in the EBES, and their abundance increased with ENR concentration, suggesting they played key roles to remove ENR and another nutrient element. The low relative abundances (1.9 ×10 -7 to 1.1 ×10 -5 copies/g) of aac (6')-ib-cr, qnrA, qnrD, qnrS, and gyrA in effluent revealed that the present EBES reactor had superior capabilities in controlling antibiotic-resistance genes and antibiotic-resistant bacteria. Our trial experiments provided a novel way for antibiotic livestock wastewater treatment.